Ocular penetration of fluorometholone-loaded PEG-PLGA nanoparticles functionalized with cell-penetrating peptides

Aim: Development of fluorometholone-loaded PEG-PLGA nanoparticles (NPs) functionalized with cellpenetrating peptides for the treatment of ocular inflammatory disorders. Materials & methods: Synthesized polymers and peptides were used for elaboration of functionalized NPs, which were characterize...

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Detalles Bibliográficos
Autores: González Pizarro, Roberto Carlos, Parrotta, Graziella, Vera-García, Rodrigo, Sánchez-López, E. (Elena), Galindo Camacho, Ruth Milagros, Kjeldsen, Frank, Badía Palacín, Josefa, Baldomà Llavinés, Laura, Espina García, Marta, García López, María Luisa
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2019
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/164191
Acceso en línea:https://hdl.handle.net/2445/164191
Access Level:acceso abierto
Palabra clave:Farmacologia ocular
Agents antiinflamatoris
Sistemes d'alliberament de medicaments
Nanopartícules
Uveïtis
Polímers
Pèptids
Ocular pharmacology
Antiinflammatory agents
Drug delivery systems
Nanoparticles
Uveitis
Polymers
Peptides
Descripción
Sumario:Aim: Development of fluorometholone-loaded PEG-PLGA nanoparticles (NPs) functionalized with cellpenetrating peptides for the treatment of ocular inflammatory disorders. Materials & methods: Synthesized polymers and peptides were used for elaboration of functionalized NPs, which were characterized physicochemically. Cytotoxicity and ability to modulate the expression of proinflammatory cytokines were evaluated in vitro using human corneal epithelial cells (HCE-2). NP uptake was assayed in both in vitro and in vivo models. Results: NPs showed physicochemical characteristics suitable for ocular administration without evidence of cytotoxicity. TAT-NPs and G2-NPs were internalized and displayed anti-inflammatory activity in both HCE-2 cells and mouse eye. Conclusion: TAT-NPs and G2-NPs could be considered a novel strategy for the treatment of ocular inflammatory diseases of the anterior and posterior segment.

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