Changes in the Expression Profile of the Meiosis-Involved Mismatch Repair Genes in Impaired Human Spermatogenesis

DNA mismatch repair (MMR) genes have been described to participate in crossover events during meiotic recombination, which is, in turn, a key step of spermatogenesis. This evidence suggests that MMR family gene expression may be altered in infertile men with defective sperm production. In order to d...

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Authors: Terribas Pérez, Ernest, Bonache, Sandra, Garcia Arevalo, Marta, Sanchez, Josvany, Franco, Eladio, Bassas, Lluís, Larriba, Sara
Format: article
Status:Versión aceptada para publicación
Publication Date:2010
Country:España
Institution:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repository:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/186672
Online Access:https://hdl.handle.net/2445/186672
Access Level:Open access
Keyword:Meiosi
Espermatogènesi
Meiosis
Spermatogenesis
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spelling Changes in the Expression Profile of the Meiosis-Involved Mismatch Repair Genes in Impaired Human SpermatogenesisTerribas Pérez, ErnestBonache, SandraGarcia Arevalo, MartaSanchez, JosvanyFranco, EladioBassas, LluísLarriba, SaraMeiosiEspermatogènesiMeiosisSpermatogenesisDNA mismatch repair (MMR) genes have been described to participate in crossover events during meiotic recombination, which is, in turn, a key step of spermatogenesis. This evidence suggests that MMR family gene expression may be altered in infertile men with defective sperm production. In order to determine the expression profile of MMR genes in impaired human spermatogenesis, we performed transcript levels analysis of MMR genes (MLH1, MLH3, PMS2, MSH4, and MSH5), and other meiosis-involved genes (ATR, HSPA2, and SYCP3) as controls, by real-time reverse transcription-polymerase chain reaction in testis from 13 patients with spermatogenic failure, 5 patients with primary germ cell tumors, and 10 controls with conserved spermatogenesis. Correlation of the expression values with the histological findings was also performed. The MMR gene expression values, with the exception of PMS2, are significantly decreased in men with spermatogenic failure. The pattern of MMR reduction correlates with the severity of damage, being maximum in maturation arrest. Specifically, expression of the testicular MSH4 gene could be useful as a surrogate marker for the presence of intratesticular elongated spermatid in patients with nonobstructive azoospermia, contributing to predict the viability of assisted reproduction. Interestingly, a reduction in the MSH4 and MSH5 transcript concentration per spermatocyte was also observed. The decreased expression level of other meiosis-specific genes, such as HSPA2 and SYCP3, suggests that the spermatocyte capacity to express meiosis-related genes is markedly reduced in spermatogenic failure, contributing to meiosis impairment and spermatogenic blockade.Wiley2022202220102022info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersion40 p.application/pdfapplication/pdfhttps://hdl.handle.net/2445/186672Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésPotprint del document publicat a: https://doi.org/10.2164/jandrol.109.008805Journal of Andrology, 2010, vol. 31, num. 4, p. 346-357https://doi.org/10.2164/jandrol.109.008805(c) American Society of Andrology, 2010info:eu-repo/semantics/openAccessoai:recercat.cat:2445/1866722026-05-29T05:05:01Z
dc.title.none.fl_str_mv Changes in the Expression Profile of the Meiosis-Involved Mismatch Repair Genes in Impaired Human Spermatogenesis
title Changes in the Expression Profile of the Meiosis-Involved Mismatch Repair Genes in Impaired Human Spermatogenesis
spellingShingle Changes in the Expression Profile of the Meiosis-Involved Mismatch Repair Genes in Impaired Human Spermatogenesis
Terribas Pérez, Ernest
Meiosi
Espermatogènesi
Meiosis
Spermatogenesis
title_short Changes in the Expression Profile of the Meiosis-Involved Mismatch Repair Genes in Impaired Human Spermatogenesis
title_full Changes in the Expression Profile of the Meiosis-Involved Mismatch Repair Genes in Impaired Human Spermatogenesis
title_fullStr Changes in the Expression Profile of the Meiosis-Involved Mismatch Repair Genes in Impaired Human Spermatogenesis
title_full_unstemmed Changes in the Expression Profile of the Meiosis-Involved Mismatch Repair Genes in Impaired Human Spermatogenesis
title_sort Changes in the Expression Profile of the Meiosis-Involved Mismatch Repair Genes in Impaired Human Spermatogenesis
dc.creator.none.fl_str_mv Terribas Pérez, Ernest
Bonache, Sandra
Garcia Arevalo, Marta
Sanchez, Josvany
Franco, Eladio
Bassas, Lluís
Larriba, Sara
author Terribas Pérez, Ernest
author_facet Terribas Pérez, Ernest
Bonache, Sandra
Garcia Arevalo, Marta
Sanchez, Josvany
Franco, Eladio
Bassas, Lluís
Larriba, Sara
author_role author
author2 Bonache, Sandra
Garcia Arevalo, Marta
Sanchez, Josvany
Franco, Eladio
Bassas, Lluís
Larriba, Sara
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Meiosi
Espermatogènesi
Meiosis
Spermatogenesis
topic Meiosi
Espermatogènesi
Meiosis
Spermatogenesis
description DNA mismatch repair (MMR) genes have been described to participate in crossover events during meiotic recombination, which is, in turn, a key step of spermatogenesis. This evidence suggests that MMR family gene expression may be altered in infertile men with defective sperm production. In order to determine the expression profile of MMR genes in impaired human spermatogenesis, we performed transcript levels analysis of MMR genes (MLH1, MLH3, PMS2, MSH4, and MSH5), and other meiosis-involved genes (ATR, HSPA2, and SYCP3) as controls, by real-time reverse transcription-polymerase chain reaction in testis from 13 patients with spermatogenic failure, 5 patients with primary germ cell tumors, and 10 controls with conserved spermatogenesis. Correlation of the expression values with the histological findings was also performed. The MMR gene expression values, with the exception of PMS2, are significantly decreased in men with spermatogenic failure. The pattern of MMR reduction correlates with the severity of damage, being maximum in maturation arrest. Specifically, expression of the testicular MSH4 gene could be useful as a surrogate marker for the presence of intratesticular elongated spermatid in patients with nonobstructive azoospermia, contributing to predict the viability of assisted reproduction. Interestingly, a reduction in the MSH4 and MSH5 transcript concentration per spermatocyte was also observed. The decreased expression level of other meiosis-specific genes, such as HSPA2 and SYCP3, suggests that the spermatocyte capacity to express meiosis-related genes is markedly reduced in spermatogenic failure, contributing to meiosis impairment and spermatogenic blockade.
publishDate 2010
dc.date.none.fl_str_mv 2010
2022
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/186672
url https://hdl.handle.net/2445/186672
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Potprint del document publicat a: https://doi.org/10.2164/jandrol.109.008805
Journal of Andrology, 2010, vol. 31, num. 4, p. 346-357
https://doi.org/10.2164/jandrol.109.008805
dc.rights.none.fl_str_mv (c) American Society of Andrology, 2010
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) American Society of Andrology, 2010
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 40 p.
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.812429

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