Substitutions in position 222 of haemagglutinin of pandemic influenza A (H1N1) 2009 viruses in Spain

Background: A change of aspartic acid (D) to glycine (G) at position 222 in the haemagglutinin (HA) protein of pandemic influenza A (H1N1) 2009 viruses was described in Norway on November 2009 with considerable frequency in fatal and severe cases. This change was detected in other countries and was...

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Detalhes bibliográficos
Autores: Ledesma, Juan, Pozo Sanchez, Francisco, Pérez-Ruiz, Mercedes, Navarro, José María, Piñeiro, Luis, Montes, Milagros, Pérez Castro, Sonia, Suárez Fernández, Jonathan, García Costa, Juan, Fernández, Mirian, Galán, Juan Carlos, Cuevas, Maria Teresa, Casas Flecha, Inmaculada, Perez-Breña, Pilar, Spanish Influenza Surveillance System (SISS), Jimenez-Jorge, Silvia
Tipo de documento: artigo
Data de publicação:2011
País:España
Recursos:Instituto de Salud Carlos III (ISCIII)
Repositório:Repisalud
Idioma:inglês
OAI Identifier:oai:repisalud.isciii.es:20.500.12105/26142
Acesso em linha:https://hdl.handle.net/20.500.12105/26142
Access Level:Acceso aberto
Palavra-chave:Pandemic influenza A (H1N1) 2009 virus
Haemagglutinin sequencing
D222G substitution
Severe respiratory infection
Adolescent
Adult
Amino Acid Substitution
Antiviral Agents
Female
Hemagglutinin Glycoproteins, Influenza Virus
Humans
Influenza A Virus, H1N1 Subtype
Influenza, Human
Male
Middle Aged
Mutation
Oseltamivir
Pandemics
Sequence Analysis, DNA
Spain
Descrição
Resumo:Background: A change of aspartic acid (D) to glycine (G) at position 222 in the haemagglutinin (HA) protein of pandemic influenza A (H1N1) 2009 viruses was described in Norway on November 2009 with considerable frequency in fatal and severe cases. This change was detected in other countries and was related only with severe disease. Other substitutions to glutamic acid (E) or asparagine (N) at position 222 were detected among pandemic viruses but it is unclear what implications might have in terms of severity. Objectives: To analyse the appearance of amino acid substitutions at position 222 in the HA protein of circulating viruses in Spain and to determine their relationships with the disease symptoms observed. Study design: Pandemic influenza A (H1N1) 2009 viruses detected in respiratory samples of 273 severe and 533 non-severe cases from different Spanish regions were selected for sequencing of a partial segment of HA1 subunit and studied to monitor substitutions at position 222. Results: D222G substitution was only detected in viruses from 14 severe cases (5.12%). D222E was found in viruses from 47 severe (17.21%) and from 52 non-severe cases (9.75%). D222N occurred in viruses from 3 additional severe cases (0.37%). Conclusion: Appearance of D222G and D222E substitution in HA of pandemic influenza A (H1N1) viruses circulating in Spain might be related with severe respiratory disease.

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