Sexually dimorphic effect of genistein on hypothalamic neuronal differentiation in vitro

© The Author(s).

Detalhes bibliográficos
Autores: Marraudino, Marilena, Farinetti, Alice, Arévalo, María Ángeles, Gotti, Stefano, Panzica, G., García-Segura, Luis M.
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/182963
Acesso em linha:http://hdl.handle.net/10261/182963
Access Level:acceso abierto
Palavra-chave:Genistein
Sex differences
Hypothalamic neurons
Neuritogenesis
Estrogen receptors
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spelling Sexually dimorphic effect of genistein on hypothalamic neuronal differentiation in vitroMarraudino, MarilenaFarinetti, AliceArévalo, María ÁngelesGotti, StefanoPanzica, G.García-Segura, Luis M.GenisteinSex differencesHypothalamic neuronsNeuritogenesisEstrogen receptors© The Author(s).Developmental actions of estradiol in the hypothalamus are well characterized. This hormone generates sex differences in the development of hypothalamic neuronal circuits controlling neuroendocrine events, feeding, growth, reproduction and behavior. In vitro, estradiol promotes sexually dimorphic effects on hypothalamic neuritogenesis. Previous studies have shown that developmental actions of the phytoestrogen genistein result in permanent sexually dimorphic effects in some behaviors and neural circuits in vivo. In the present study, we have explored if genistein, like estradiol, affects neuritogenesis in primary hypothalamic neurons and investigated the estrogen receptors implicated in this action. Hypothalamic neuronal cultures, obtained from male or female embryonic day 14 (E14) CD1 mice, were treated with genistein (0.1 µM, 0.5 µM or 1 µM) or vehicle. Under basal conditions, female neurons had longer primary neurites, higher number of secondary neurites and higher neuritic arborization compared to male neurons. The treatment with genistein increased neuritic arborization and the number of primary neurites and decreased the number of secondary neurites in female neurons, but not in male neurons. In contrast, genistein resulted in a significant increase in primary neuritic length in male neurons, but not in female neurons. The use of selective estrogen receptor antagonists suggests that estrogen receptor α, estrogen receptor β and G-protein-coupled estrogen receptors are involved in the neuritogenic action of genistein. In summary, these findings indicate that genistein exerts sexually dimorphic actions on the development of hypothalamic neurons, altering the normal pattern of sex differences in neuritogenesis.This work was supported by COST (European Cooperation in Science and Technology) Action BM1105, Fondazione Cavalieri Ottolenghi and University of Torino (S.G., G.P.), Fondazione CRT, RF 2015.2564 (S.G.), Agencia Estatal de Investigación, Spain (BFU2017-82754-R), CIBERFES and Fondos Feder. This study is part of Ministero dell’Istruzione, dell’Università e della Ricerca (MIUR) project “Dipartimenti di Eccellenza 2018 – 2022” to Department of Neuroscience “Rita Levi Montalcini”, University of Torino.Peer reviewedMultidisciplinary Digital Publishing InstituteAgencia Estatal de Investigación (España)Ministerio de Ciencia, Innovación y Universidades (España)European Cooperation in Science and TechnologyUniversità di TorinoCentro de Investigación Biomédica en Red de Fragilidad y Envejecimiento Saludable (España)European CommissionMinistero dell'Istruzione, dell'Università e della RicercaConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2019201920192019info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/182963reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#BFU2017-82754-R/AEI/10.13039/501100011033info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/BFU2017-82754-Rhttps://doi.org/10.3390/ijms20102465Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1829632026-05-22T06:33:51Z
dc.title.none.fl_str_mv Sexually dimorphic effect of genistein on hypothalamic neuronal differentiation in vitro
title Sexually dimorphic effect of genistein on hypothalamic neuronal differentiation in vitro
spellingShingle Sexually dimorphic effect of genistein on hypothalamic neuronal differentiation in vitro
Marraudino, Marilena
Genistein
Sex differences
Hypothalamic neurons
Neuritogenesis
Estrogen receptors
title_short Sexually dimorphic effect of genistein on hypothalamic neuronal differentiation in vitro
title_full Sexually dimorphic effect of genistein on hypothalamic neuronal differentiation in vitro
title_fullStr Sexually dimorphic effect of genistein on hypothalamic neuronal differentiation in vitro
title_full_unstemmed Sexually dimorphic effect of genistein on hypothalamic neuronal differentiation in vitro
title_sort Sexually dimorphic effect of genistein on hypothalamic neuronal differentiation in vitro
dc.creator.none.fl_str_mv Marraudino, Marilena
Farinetti, Alice
Arévalo, María Ángeles
Gotti, Stefano
Panzica, G.
García-Segura, Luis M.
author Marraudino, Marilena
author_facet Marraudino, Marilena
Farinetti, Alice
Arévalo, María Ángeles
Gotti, Stefano
Panzica, G.
García-Segura, Luis M.
author_role author
author2 Farinetti, Alice
Arévalo, María Ángeles
Gotti, Stefano
Panzica, G.
García-Segura, Luis M.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Agencia Estatal de Investigación (España)
Ministerio de Ciencia, Innovación y Universidades (España)
European Cooperation in Science and Technology
Università di Torino
Centro de Investigación Biomédica en Red de Fragilidad y Envejecimiento Saludable (España)
European Commission
Ministero dell'Istruzione, dell'Università e della Ricerca
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Genistein
Sex differences
Hypothalamic neurons
Neuritogenesis
Estrogen receptors
topic Genistein
Sex differences
Hypothalamic neurons
Neuritogenesis
Estrogen receptors
description © The Author(s).
publishDate 2019
dc.date.none.fl_str_mv 2019
2019
2019
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/182963
url http://hdl.handle.net/10261/182963
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
BFU2017-82754-R/AEI/10.13039/501100011033
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/BFU2017-82754-R
https://doi.org/10.3390/ijms20102465
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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