Highly variable Vitis microsatellite loci for the identification of Pinot Noir clones

Nineteen new microsatellite loci of Vitis were elaborated by following the procedure of tagging an SSR-enriched library. Primers for these VRG markers were used for genotyping grapevines. Only the markers VRG 1, VRG 2, VRG 4, VRG 7, VRG 9, VRG 10, VRG 15 and VRG 16 show heterozygous alleles and Mend...

Full description

Bibliographic Details
Authors: Regner, Ferdinand, Hack, R., Santiago Blanco, José Luis
Format: article
Publication Date:2006
Country:España
Institution:Consejo Superior de Investigaciones Científicas (CSIC)
Repository:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/43173
Online Access:http://hdl.handle.net/10261/43173
Access Level:Open access
Keyword:Vitis
Clonal selection
Genotyping
Pinot Noir
id ES_37892bc02c31bbacce1efa94bf19f118
oai_identifier_str oai:digital.csic.es:10261/43173
network_acronym_str ES
network_name_str España
repository_id_str
spelling Highly variable Vitis microsatellite loci for the identification of Pinot Noir clonesRegner, FerdinandHack, R.Santiago Blanco, José LuisVitisClonal selectionGenotypingPinot NoirNineteen new microsatellite loci of Vitis were elaborated by following the procedure of tagging an SSR-enriched library. Primers for these VRG markers were used for genotyping grapevines. Only the markers VRG 1, VRG 2, VRG 4, VRG 7, VRG 9, VRG 10, VRG 15 and VRG 16 show heterozygous alleles and Mendelian segregation. Other VRG loci such as VRG 5, VRG 6, VRG 11, VRG 12 VRG 13 and VRG 17 produce a multiallelic profile and some of them show distorted segregation. Variability of the VRG loci is rather high as compared to other grapevine SSR markers. Stable VRG markers such as VRG 16 can be useful for the identification of cultivars. Highly variable VRG microsatellites could be successfully applied to trace polymorphism within the variety Pinot Noir. Clones of Pinot Noir could be differentiated using these markers. By applying the PhyQuest program, a dendrogramm showing the genetic divergence within Pinot Noir clones was constructed.This project was funded by the Ministry of Agriculture and Forestry in Vienna. J. L. SANTIAGO is funded by a grant from Ministerio de Educación y Ciencia (Spain).Peer reviewedJulius Kühn-Institut201120112006info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/43173reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://www.vitis-vea.deinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/431732026-05-22T06:33:51Z
dc.title.none.fl_str_mv Highly variable Vitis microsatellite loci for the identification of Pinot Noir clones
title Highly variable Vitis microsatellite loci for the identification of Pinot Noir clones
spellingShingle Highly variable Vitis microsatellite loci for the identification of Pinot Noir clones
Regner, Ferdinand
Vitis
Clonal selection
Genotyping
Pinot Noir
title_short Highly variable Vitis microsatellite loci for the identification of Pinot Noir clones
title_full Highly variable Vitis microsatellite loci for the identification of Pinot Noir clones
title_fullStr Highly variable Vitis microsatellite loci for the identification of Pinot Noir clones
title_full_unstemmed Highly variable Vitis microsatellite loci for the identification of Pinot Noir clones
title_sort Highly variable Vitis microsatellite loci for the identification of Pinot Noir clones
dc.creator.none.fl_str_mv Regner, Ferdinand
Hack, R.
Santiago Blanco, José Luis
author Regner, Ferdinand
author_facet Regner, Ferdinand
Hack, R.
Santiago Blanco, José Luis
author_role author
author2 Hack, R.
Santiago Blanco, José Luis
author2_role author
author
dc.subject.none.fl_str_mv Vitis
Clonal selection
Genotyping
Pinot Noir
topic Vitis
Clonal selection
Genotyping
Pinot Noir
description Nineteen new microsatellite loci of Vitis were elaborated by following the procedure of tagging an SSR-enriched library. Primers for these VRG markers were used for genotyping grapevines. Only the markers VRG 1, VRG 2, VRG 4, VRG 7, VRG 9, VRG 10, VRG 15 and VRG 16 show heterozygous alleles and Mendelian segregation. Other VRG loci such as VRG 5, VRG 6, VRG 11, VRG 12 VRG 13 and VRG 17 produce a multiallelic profile and some of them show distorted segregation. Variability of the VRG loci is rather high as compared to other grapevine SSR markers. Stable VRG markers such as VRG 16 can be useful for the identification of cultivars. Highly variable VRG microsatellites could be successfully applied to trace polymorphism within the variety Pinot Noir. Clones of Pinot Noir could be differentiated using these markers. By applying the PhyQuest program, a dendrogramm showing the genetic divergence within Pinot Noir clones was constructed.
publishDate 2006
dc.date.none.fl_str_mv 2006
2011
2011
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/43173
url http://hdl.handle.net/10261/43173
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://www.vitis-vea.de
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Julius Kühn-Institut
publisher.none.fl_str_mv Julius Kühn-Institut
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869406043723792384
score 15,808905

Similar Items