Multi-omics analysis of zebrafish response to tick saliva reveals biological processes associated with alpha-Gal syndrome

The alpha-Gal syndrome (AGS) is a tick-borne allergy. A multi-omics approach was used to determine the effect of tick saliva and mammalian meat consumption on zebrafish gut transcriptome and proteome. Bioinformatics analysis using R software was focused on significant biological and metabolic pathwa...

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Autores: Vaz Rodrigues, Rita, Mazuecos, Lorena, Villar, Margarita, Contreras, Marinela, Artigas-Jerónimo, Sara, González-García, Almudena, Gortázar, Christian, Fuente, José de la
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/340576
Acceso en línea:http://hdl.handle.net/10261/340576
Access Level:acceso abierto
Palabra clave:Alpha-Gal syndrome
Danio rerio
Multi-omics
Transcriptomics
Proteomics
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spelling Multi-omics analysis of zebrafish response to tick saliva reveals biological processes associated with alpha-Gal syndromeVaz Rodrigues, RitaMazuecos, LorenaVillar, MargaritaContreras, MarinelaArtigas-Jerónimo, SaraGonzález-García, AlmudenaGortázar, ChristianFuente, José de laAlpha-Gal syndromeDanio rerioMulti-omicsTranscriptomicsProteomicsThe alpha-Gal syndrome (AGS) is a tick-borne allergy. A multi-omics approach was used to determine the effect of tick saliva and mammalian meat consumption on zebrafish gut transcriptome and proteome. Bioinformatics analysis using R software was focused on significant biological and metabolic pathway changes associated with AGS. Ortholog mapping identified highly concordant human ortholog genes for the detection of disease-enriched pathways. Tick saliva treatment increased zebrafish mortality, incidence of hemorrhagic type allergic reactions and changes in behavior and feeding patterns. Transcriptomics analysis showed downregulation of biological and metabolic pathways correlated with anti-alpha-Gal IgE and allergic reactions to tick saliva affecting blood circulation, cardiac and vascular smooth muscle contraction, behavior and sensory perception. Disease enrichment analysis revealed downregulated orthologous genes associated with human disorders affecting nervous, musculoskeletal, and cardiovascular systems. Proteomics analysis revealed suppression of pathways associated with immune system production of reactive oxygen species and cardiac muscle contraction. Underrepresented proteins were mainly linked to nervous and metabolic human disorders. Multi-omics data revealed inhibition of pathways associated with adrenergic signaling in cardiomyocytes, and heart and muscle contraction. Results identify tick saliva-related biological pathways supporting multisystemic organ involvement and linking α-Gal sensitization with other illnesses for the identification of potential disease biomarkers.This work was supported by Ministerio de Ciencia e Innovación/Agencia Estatal de Investigación MCIN/AEI/ 10.13039/501100011033, Spain and EU-FEDER [grant BIOGAL PID2020116761GB-I00]. R. Vaz-Rodrigues was funded by Universidad de Castilla-La Mancha (UCLM), Spain and the European Social Fund (ESF) [doctoral contract 2022-PRED-20675]. L. Mazuecos was supported by UCLM co-financed by ESF [post-doctoral grant 2021-POST-32002]. M. Contreras was funded by Ministerio de Ciencia, Innovación y Universidades, Spain [post-doctoral grant IJC2020-042710-I].Peer reviewedElsevierMinisterio de Ciencia, Innovación y Universidades (España)Agencia Estatal de Investigación (España)European CommissionUniversidad de Castilla La ManchaConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232023info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/340576reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-116761GB-I00info:eu-repo/grantAgreement/AEI//IJC2020-042710-IThe underlying dataset has been published as supplementary material of the article in the publisher platform at DOI 10.1016/j.biopha.2023.115829https://doi.org/10.1016/j.biopha.2023.115829Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3405762026-05-22T06:33:51Z
dc.title.none.fl_str_mv Multi-omics analysis of zebrafish response to tick saliva reveals biological processes associated with alpha-Gal syndrome
title Multi-omics analysis of zebrafish response to tick saliva reveals biological processes associated with alpha-Gal syndrome
spellingShingle Multi-omics analysis of zebrafish response to tick saliva reveals biological processes associated with alpha-Gal syndrome
Vaz Rodrigues, Rita
Alpha-Gal syndrome
Danio rerio
Multi-omics
Transcriptomics
Proteomics
title_short Multi-omics analysis of zebrafish response to tick saliva reveals biological processes associated with alpha-Gal syndrome
title_full Multi-omics analysis of zebrafish response to tick saliva reveals biological processes associated with alpha-Gal syndrome
title_fullStr Multi-omics analysis of zebrafish response to tick saliva reveals biological processes associated with alpha-Gal syndrome
title_full_unstemmed Multi-omics analysis of zebrafish response to tick saliva reveals biological processes associated with alpha-Gal syndrome
title_sort Multi-omics analysis of zebrafish response to tick saliva reveals biological processes associated with alpha-Gal syndrome
dc.creator.none.fl_str_mv Vaz Rodrigues, Rita
Mazuecos, Lorena
Villar, Margarita
Contreras, Marinela
Artigas-Jerónimo, Sara
González-García, Almudena
Gortázar, Christian
Fuente, José de la
author Vaz Rodrigues, Rita
author_facet Vaz Rodrigues, Rita
Mazuecos, Lorena
Villar, Margarita
Contreras, Marinela
Artigas-Jerónimo, Sara
González-García, Almudena
Gortázar, Christian
Fuente, José de la
author_role author
author2 Mazuecos, Lorena
Villar, Margarita
Contreras, Marinela
Artigas-Jerónimo, Sara
González-García, Almudena
Gortázar, Christian
Fuente, José de la
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia, Innovación y Universidades (España)
Agencia Estatal de Investigación (España)
European Commission
Universidad de Castilla La Mancha
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Alpha-Gal syndrome
Danio rerio
Multi-omics
Transcriptomics
Proteomics
topic Alpha-Gal syndrome
Danio rerio
Multi-omics
Transcriptomics
Proteomics
description The alpha-Gal syndrome (AGS) is a tick-borne allergy. A multi-omics approach was used to determine the effect of tick saliva and mammalian meat consumption on zebrafish gut transcriptome and proteome. Bioinformatics analysis using R software was focused on significant biological and metabolic pathway changes associated with AGS. Ortholog mapping identified highly concordant human ortholog genes for the detection of disease-enriched pathways. Tick saliva treatment increased zebrafish mortality, incidence of hemorrhagic type allergic reactions and changes in behavior and feeding patterns. Transcriptomics analysis showed downregulation of biological and metabolic pathways correlated with anti-alpha-Gal IgE and allergic reactions to tick saliva affecting blood circulation, cardiac and vascular smooth muscle contraction, behavior and sensory perception. Disease enrichment analysis revealed downregulated orthologous genes associated with human disorders affecting nervous, musculoskeletal, and cardiovascular systems. Proteomics analysis revealed suppression of pathways associated with immune system production of reactive oxygen species and cardiac muscle contraction. Underrepresented proteins were mainly linked to nervous and metabolic human disorders. Multi-omics data revealed inhibition of pathways associated with adrenergic signaling in cardiomyocytes, and heart and muscle contraction. Results identify tick saliva-related biological pathways supporting multisystemic organ involvement and linking α-Gal sensitization with other illnesses for the identification of potential disease biomarkers.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/340576
url http://hdl.handle.net/10261/340576
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-116761GB-I00
info:eu-repo/grantAgreement/AEI//IJC2020-042710-I
The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI 10.1016/j.biopha.2023.115829
https://doi.org/10.1016/j.biopha.2023.115829
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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