Mechanism of RanGTP dependent microtubule assembly during mitosis
During mitosis, spindle assembly involves different sources of microtubules including centrosomes and chromosomes. While the role of centrosomes has been extensively studied, we still do not fully understand how chromosomes trigger microtubule assembly thereby contributing to the formation of the mi...
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| Tipo de recurso: | tesis doctoral |
| Estado: | Versión publicada |
| Fecha de publicación: | 2014 |
| País: | España |
| Institución: | CBUC, CESCA |
| Repositorio: | TDR. Tesis Doctorales en Red |
| OAI Identifier: | oai:www.tdx.cat:10803/289621 |
| Acceso en línea: | http://hdl.handle.net/10803/289621 |
| Access Level: | acceso abierto |
| Palabra clave: | Cell Cell cycle Mitosis Cell division Microtubule Mitotic spindle Chromosomes RanGTP Nucleation ϒ-tubulin TPX2 RHAMM NEDD1 Aurora-A Xenopus laevis Phosphorylation Cicle cel·lular Divisió cel·lular Tubulina Microtúbuls Fus acromàtic Ensamblatge del fús 576 |
| Sumario: | During mitosis, spindle assembly involves different sources of microtubules including centrosomes and chromosomes. While the role of centrosomes has been extensively studied, we still do not fully understand how chromosomes trigger microtubule assembly thereby contributing to the formation of the mitotic spindle. The chromosomal pathway is largely determined by a RanGTP gradient centered on the chromosomes that induces the local activation of spindle assembly factors. To get a better understanding on the RanGTP-dependent microtubule assembly during mitosis we aimed at: i) Identifying new RanGTP regulated proteins involved in spindle assembly. Our results pointed to three novel proteins with a putative mitotic role in the RanGTP pathway. ii) Understanding how the RanGTP pathway regulates microtubule nucleation during mitosis. We found that TPX2 together with Aurora-A and RHAMM are part of a RanGTP-dependent complex that binds and strongly stimulates the -TuRC nucleation activity. iii) Investigating the contribution of the RanGTP pathway to spindle assembly. Our data provided novel evidences that MTs nucleated close to the chromosomes participate in the k-fibers assembly process. |
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