RNA binding of T-cell intracellular antigen-1 (TIA-1) C-terminal RNA recognition motif is modified by pH conditions

Background: T-cell intracellular antigen- 1(TIA-1) is a key DNA/RNA binding protein in RNA metabolism. Results: The binding of TIA-1 RRM3 domain (isolated or fused to RRM2) to RNA is affected by pH changes. Conclusion: The pH-dependence of RRM3/RNA interaction may have a significant effect in the ov...

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Detalhes bibliográficos
Autores: Cruz-Gallardo, Isabel, Aroca, Ángeles, Persson, Cecilia, Karlsson, B. Göran, Díaz-Moreno, Irene
Tipo de documento: artigo
Data de publicação:2013
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositório:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/95466
Acesso em linha:http://hdl.handle.net/10261/95466
Access Level:Acceso aberto
Palavra-chave:DNA/RNA binding protein (D/RBP)
pH-dependence
NMR
RNA recognition motif (RRM)
T-cell intracellular antigen-1 (TIA-1)
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network_acronym_str ES
network_name_str España
repository_id_str
spelling RNA binding of T-cell intracellular antigen-1 (TIA-1) C-terminal RNA recognition motif is modified by pH conditionsCruz-Gallardo, IsabelAroca, ÁngelesPersson, CeciliaKarlsson, B. GöranDíaz-Moreno, IreneDNA/RNA binding protein (D/RBP)pH-dependenceNMRRNA recognition motif (RRM)T-cell intracellular antigen-1 (TIA-1)Background: T-cell intracellular antigen- 1(TIA-1) is a key DNA/RNA binding protein in RNA metabolism. Results: The binding of TIA-1 RRM3 domain (isolated or fused to RRM2) to RNA is affected by pH changes. Conclusion: The pH-dependence of RRM3/RNA interaction may have a significant effect in the overall TIA-1 function. Significance: Unveiling the auxiliary role of RRM3 in RNA recognition sheds light on the TIA-1 function between cell compartments changes due to the protonation / deprotonation of TIA-1 RRM3 histidine residues. The auxiliary role of the Cterminal RRM3 domain in TIA-1 RNA recognition is poorly understood and this work provides an insight into its binding mechanismsPeer reviewedAmerican Society for Biochemistry and Molecular Biology201420142013info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/95466reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Gallegohttp://dx.doi.org/10.1074/jbc.M113.489070info:eu-repo/semantics/openAccessoai:digital.csic.es:10261/954662026-05-22T06:33:51Z
dc.title.none.fl_str_mv RNA binding of T-cell intracellular antigen-1 (TIA-1) C-terminal RNA recognition motif is modified by pH conditions
title RNA binding of T-cell intracellular antigen-1 (TIA-1) C-terminal RNA recognition motif is modified by pH conditions
spellingShingle RNA binding of T-cell intracellular antigen-1 (TIA-1) C-terminal RNA recognition motif is modified by pH conditions
Cruz-Gallardo, Isabel
DNA/RNA binding protein (D/RBP)
pH-dependence
NMR
RNA recognition motif (RRM)
T-cell intracellular antigen-1 (TIA-1)
title_short RNA binding of T-cell intracellular antigen-1 (TIA-1) C-terminal RNA recognition motif is modified by pH conditions
title_full RNA binding of T-cell intracellular antigen-1 (TIA-1) C-terminal RNA recognition motif is modified by pH conditions
title_fullStr RNA binding of T-cell intracellular antigen-1 (TIA-1) C-terminal RNA recognition motif is modified by pH conditions
title_full_unstemmed RNA binding of T-cell intracellular antigen-1 (TIA-1) C-terminal RNA recognition motif is modified by pH conditions
title_sort RNA binding of T-cell intracellular antigen-1 (TIA-1) C-terminal RNA recognition motif is modified by pH conditions
dc.creator.none.fl_str_mv Cruz-Gallardo, Isabel
Aroca, Ángeles
Persson, Cecilia
Karlsson, B. Göran
Díaz-Moreno, Irene
author Cruz-Gallardo, Isabel
author_facet Cruz-Gallardo, Isabel
Aroca, Ángeles
Persson, Cecilia
Karlsson, B. Göran
Díaz-Moreno, Irene
author_role author
author2 Aroca, Ángeles
Persson, Cecilia
Karlsson, B. Göran
Díaz-Moreno, Irene
author2_role author
author
author
author
dc.subject.none.fl_str_mv DNA/RNA binding protein (D/RBP)
pH-dependence
NMR
RNA recognition motif (RRM)
T-cell intracellular antigen-1 (TIA-1)
topic DNA/RNA binding protein (D/RBP)
pH-dependence
NMR
RNA recognition motif (RRM)
T-cell intracellular antigen-1 (TIA-1)
description Background: T-cell intracellular antigen- 1(TIA-1) is a key DNA/RNA binding protein in RNA metabolism. Results: The binding of TIA-1 RRM3 domain (isolated or fused to RRM2) to RNA is affected by pH changes. Conclusion: The pH-dependence of RRM3/RNA interaction may have a significant effect in the overall TIA-1 function. Significance: Unveiling the auxiliary role of RRM3 in RNA recognition sheds light on the TIA-1 function between cell compartments changes due to the protonation / deprotonation of TIA-1 RRM3 histidine residues. The auxiliary role of the Cterminal RRM3 domain in TIA-1 RNA recognition is poorly understood and this work provides an insight into its binding mechanisms
publishDate 2013
dc.date.none.fl_str_mv 2013
2014
2014
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/95466
url http://hdl.handle.net/10261/95466
dc.language.none.fl_str_mv Gallego
language_invalid_str_mv Gallego
dc.relation.none.fl_str_mv http://dx.doi.org/10.1074/jbc.M113.489070
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Society for Biochemistry and Molecular Biology
publisher.none.fl_str_mv American Society for Biochemistry and Molecular Biology
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.812429

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