Biocatalytic Production of Solketal Esters from Used Oil Utilizing Treated Macauba Epicarp Particles as Lipase Immobilization Support: A Dual Valorization of Wastes for Sustainable Chemistry

This study describes the production of solketal esters from used soybean cooking oil (USCO) via enzymatic hydroesterification. This process consists of the complete hydrolysis of USCO into free fatty acids (FFAs) catalyzed by crude lipase extract from Candida rugosa (CRL). The resulting FFAs were re...

ver descrição completa

Detalhes bibliográficos
Autores: Miguel Júnior, José, Dimas, João V. B., Barbosa, Milson S., Gomes, Raphael A. B., Carvalho, Ana K. F., Soares, Cleide M. F., Fernández-Lafuente, Roberto, Mendes, Adriano A
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/370767
Acesso em linha:http://hdl.handle.net/10261/370767
Access Level:acceso abierto
Palavra-chave:Solketal esters
Hydroesterification
Lipase
Agro-industrial wastes
Valorization
id ES_34db232162e3d8eaa58cc5d3bfb20fde
oai_identifier_str oai:digital.csic.es:10261/370767
network_acronym_str ES
network_name_str España
repository_id_str
spelling Biocatalytic Production of Solketal Esters from Used Oil Utilizing Treated Macauba Epicarp Particles as Lipase Immobilization Support: A Dual Valorization of Wastes for Sustainable ChemistryMiguel Júnior, JoséDimas, João V. B.Barbosa, Milson S.Gomes, Raphael A. B.Carvalho, Ana K. F.Soares, Cleide M. F.Fernández-Lafuente, RobertoMendes, Adriano ASolketal estersHydroesterificationLipaseAgro-industrial wastesValorizationThis study describes the production of solketal esters from used soybean cooking oil (USCO) via enzymatic hydroesterification. This process consists of the complete hydrolysis of USCO into free fatty acids (FFAs) catalyzed by crude lipase extract from Candida rugosa (CRL). The resulting FFAs were recovered and utilized as the raw material for an esterification reaction with solketal, which was achieved via an open reaction. For this purpose, lipase Eversa® Transform 2.0 (ET2.0) was immobilized via physical adsorption on treated epicarp particles from Acrocomia aculeata (macauba), a lignocellulosic residue. A protein loading of 25.2 ± 1.3 mg g−1 with a support and immobilization yield of 64.8 ± 2.5% was achieved using an initial protein loading of 40 mg g−1 of support. The influence of certain parameters on the esterification reaction was evaluated using a central composite rotatable design (CCRD). Under optimal conditions, a FFAs conversion of 72.5 ± 0.8% was obtained after 150 min of reaction at 46 ◦C using a biocatalyst concentration of 20% wt. and a FFAs–solketal molar ratio of 1:1.6. The biocatalyst retained 70% of its original activity after ten esterification batches. This paper shows the conversion of two agro-industrial waste into valuable materials (enzyme immobilization support and solketal esters)The present study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)—Brazil—Finance Code 001. The authors are also thankful for the financial support of Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)—Brazil (Process APQ-01691-21) and Ministerio de Ciencia e Innovación, and Agencia Estatal de Investigación (Spanish Government) (PID2022-136535OB-I00). A.A.M. thanks the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) for the research fellowship (PQ-2 CA BI, Grant 306253/2023-2). J.M.J. and J.V.B.D. thank the FAPEMIG and CNPq Foundation for the student fellowships, respectivelyPeer reviewedMultidisciplinary Digital Publishing InstituteCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (Brasil)Fundação de Amparo à Pesquisa do Estado de São Paulo Minas GeraisMinisterio de Ciencia e Innovación (España)Agencia Estatal de Investigación (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2024202420242024info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/370767reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2022-136535OB-I00The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI https://doi.org/10.3390/catal14100693https://doi.org/10.3390/catal14100693Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3707672026-05-22T06:33:51Z
dc.title.none.fl_str_mv Biocatalytic Production of Solketal Esters from Used Oil Utilizing Treated Macauba Epicarp Particles as Lipase Immobilization Support: A Dual Valorization of Wastes for Sustainable Chemistry
title Biocatalytic Production of Solketal Esters from Used Oil Utilizing Treated Macauba Epicarp Particles as Lipase Immobilization Support: A Dual Valorization of Wastes for Sustainable Chemistry
spellingShingle Biocatalytic Production of Solketal Esters from Used Oil Utilizing Treated Macauba Epicarp Particles as Lipase Immobilization Support: A Dual Valorization of Wastes for Sustainable Chemistry
Miguel Júnior, José
Solketal esters
Hydroesterification
Lipase
Agro-industrial wastes
Valorization
title_short Biocatalytic Production of Solketal Esters from Used Oil Utilizing Treated Macauba Epicarp Particles as Lipase Immobilization Support: A Dual Valorization of Wastes for Sustainable Chemistry
title_full Biocatalytic Production of Solketal Esters from Used Oil Utilizing Treated Macauba Epicarp Particles as Lipase Immobilization Support: A Dual Valorization of Wastes for Sustainable Chemistry
title_fullStr Biocatalytic Production of Solketal Esters from Used Oil Utilizing Treated Macauba Epicarp Particles as Lipase Immobilization Support: A Dual Valorization of Wastes for Sustainable Chemistry
title_full_unstemmed Biocatalytic Production of Solketal Esters from Used Oil Utilizing Treated Macauba Epicarp Particles as Lipase Immobilization Support: A Dual Valorization of Wastes for Sustainable Chemistry
title_sort Biocatalytic Production of Solketal Esters from Used Oil Utilizing Treated Macauba Epicarp Particles as Lipase Immobilization Support: A Dual Valorization of Wastes for Sustainable Chemistry
dc.creator.none.fl_str_mv Miguel Júnior, José
Dimas, João V. B.
Barbosa, Milson S.
Gomes, Raphael A. B.
Carvalho, Ana K. F.
Soares, Cleide M. F.
Fernández-Lafuente, Roberto
Mendes, Adriano A
author Miguel Júnior, José
author_facet Miguel Júnior, José
Dimas, João V. B.
Barbosa, Milson S.
Gomes, Raphael A. B.
Carvalho, Ana K. F.
Soares, Cleide M. F.
Fernández-Lafuente, Roberto
Mendes, Adriano A
author_role author
author2 Dimas, João V. B.
Barbosa, Milson S.
Gomes, Raphael A. B.
Carvalho, Ana K. F.
Soares, Cleide M. F.
Fernández-Lafuente, Roberto
Mendes, Adriano A
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Brasil)
Fundação de Amparo à Pesquisa do Estado de São Paulo Minas Gerais
Ministerio de Ciencia e Innovación (España)
Agencia Estatal de Investigación (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Solketal esters
Hydroesterification
Lipase
Agro-industrial wastes
Valorization
topic Solketal esters
Hydroesterification
Lipase
Agro-industrial wastes
Valorization
description This study describes the production of solketal esters from used soybean cooking oil (USCO) via enzymatic hydroesterification. This process consists of the complete hydrolysis of USCO into free fatty acids (FFAs) catalyzed by crude lipase extract from Candida rugosa (CRL). The resulting FFAs were recovered and utilized as the raw material for an esterification reaction with solketal, which was achieved via an open reaction. For this purpose, lipase Eversa® Transform 2.0 (ET2.0) was immobilized via physical adsorption on treated epicarp particles from Acrocomia aculeata (macauba), a lignocellulosic residue. A protein loading of 25.2 ± 1.3 mg g−1 with a support and immobilization yield of 64.8 ± 2.5% was achieved using an initial protein loading of 40 mg g−1 of support. The influence of certain parameters on the esterification reaction was evaluated using a central composite rotatable design (CCRD). Under optimal conditions, a FFAs conversion of 72.5 ± 0.8% was obtained after 150 min of reaction at 46 ◦C using a biocatalyst concentration of 20% wt. and a FFAs–solketal molar ratio of 1:1.6. The biocatalyst retained 70% of its original activity after ten esterification batches. This paper shows the conversion of two agro-industrial waste into valuable materials (enzyme immobilization support and solketal esters)
publishDate 2024
dc.date.none.fl_str_mv 2024
2024
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/370767
url http://hdl.handle.net/10261/370767
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2022-136535OB-I00
The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI https://doi.org/10.3390/catal14100693
https://doi.org/10.3390/catal14100693
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869405844143079424
score 15,811543

Registos relacionados