Immobilized Lipases in the Synthesis of Short-Chain Esters: An Overview of Constraints and Perspectives

Biocatalysis—specifically the use of immobilized lipases—has been proposed as a greener alternative for ester production. Several critical challenges, such as the high cost of biocatalysts, are delaying the industrial implementation of biocatalysis. Moreover, for short-chain ester synthesis, the str...

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Detalles Bibliográficos
Autores: Sousa, Ronaldo Rodrigues de, Santos, Michelle M. dos, Medeiros, Matheus W. R., Manoel, Evelin A., Berenguer Murcia, A., Freire, Denise Maria Guimarães, Fernández-Lafuente, Roberto, Ferreira-Leitão, Viridiana Santana
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/391253
Acceso en línea:http://hdl.handle.net/10261/391253
Access Level:acceso abierto
Palabra clave:Esters
Biocatalysis
Lipases
Immobilized enzymes
Short-chain esters
Descripción
Sumario:Biocatalysis—specifically the use of immobilized lipases—has been proposed as a greener alternative for ester production. Several critical challenges, such as the high cost of biocatalysts, are delaying the industrial implementation of biocatalysis. Moreover, for short-chain ester synthesis, the strong inhibition/inactivation potential of short-chain acids and alcohols on lipases leads to long reaction cycles and/or the need to use organic solvents to overcome the limitations of solvent-free systems and, consequently, the decrease in product concentrations. This review presents an overview of the scientific developments in enzymatic short-chain ester synthesis, compiling the constraints on their syntheses from a process perspective, including insights about key performance indicators (KPI) and economic parameters.