Development of a versatile biotinylated material based on SU-8

Ortega Higueruelo, Francisco José; Sanza, Francisco J.; Laguna, Mari Fe; Holgado Bolaños, Miguel; Casquel Del Campo, Rafael; Angulo Barrios, Carlos; López Romero, David; Bañuls Polo, María-José|||0000-0002-2422-7731; Maquieira, Angel|||0000-0003-4641-4957; Puchades, Rosa|||0000-0002-9329-1593

Development of a versatile biotinylated material based on SU-8

[EN] The negative epoxy-based SU-8 photoresist has a wide variety of applications within the semiconductor industry, photonics and lab-on-a-chip devices, and it is emerging as an alternative to silicon-based devices for sensing purposes. In the present work, biotinylation of the SU-8 polymer surface...

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Autores:	Ortega Higueruelo, Francisco José, Sanza, Francisco J., Laguna, Mari Fe, Holgado Bolaños, Miguel, Casquel Del Campo, Rafael, Angulo Barrios, Carlos, López Romero, David, Bañuls Polo, María-José\|\|\|0000-0002-2422-7731, Maquieira, Angel\|\|\|0000-0003-4641-4957, Puchades, Rosa\|\|\|0000-0002-9329-1593
Tipo de recurso:	artículo
Fecha de publicación:	2013
País:	España
Institución:	Universitat Politècnica de València (UPV)
Repositorio:	RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
Idioma:	inglés
OAI Identifier:	oai:riunet.upv.es:10251/65816
Acceso en línea:	https://riunet.upv.es/handle/10251/65816
Access Level:	acceso abierto
Palabra clave:	IMMOBILIZATION PHOTORESIST SURFACE MODIFICATION BIOMOLECULES MEMS ASSAYS DNA PHOTOLITHOGRAPHY MICROSTRUCTURES FABRICATION QUIMICA ANALITICA

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spelling	Development of a versatile biotinylated material based on SU-8Ortega Higueruelo, Francisco JoséSanza, Francisco J.Laguna, Mari FeHolgado Bolaños, MiguelCasquel Del Campo, RafaelAngulo Barrios, CarlosLópez Romero, DavidBañuls Polo, María-José\|\|\|0000-0002-2422-7731Maquieira, Angel\|\|\|0000-0003-4641-4957Puchades, Rosa\|\|\|0000-0002-9329-1593IMMOBILIZATIONPHOTORESISTSURFACE MODIFICATIONBIOMOLECULESMEMSASSAYSDNAPHOTOLITHOGRAPHYMICROSTRUCTURESFABRICATIONQUIMICA ANALITICA[EN] The negative epoxy-based SU-8 photoresist has a wide variety of applications within the semiconductor industry, photonics and lab-on-a-chip devices, and it is emerging as an alternative to silicon-based devices for sensing purposes. In the present work, biotinylation of the SU-8 polymer surface promoted by light is reported. As a result, a novel, effective, and low-cost material, focusing on the immobilization of bioreceptors and consequent biosensing, is developed. This material allows the spatial discrimination depending on the irradiation of desired areas. The most salient feature is that the photobiotin may be directly incorporated into the SU-8 curing process, consequently reducing time and cost. The potential use of this substrate is demonstrated by the immunoanalytical detection of the synthetic steroid gestrinone, showing excellent performances. Moreover, the naked eye biodetection due to the transparent SU-8 substrate, and simple instrumental quantification are additional advantages.Financial support from Ministerio de Economia y Competitividad (MINECO TEC2012-31145) is acknowledged.Royal Society of ChemistryDepartamento de QuímicaInstituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo TecnológicoEscuela Técnica Superior de Ingeniería Agronómica y del Medio NaturalMinisterio de Economía y CompetitividadRepositorio Institucional de la Universitat Politècnica de València Riunet20132013-01-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfapplication/pdfhttps://riunet.upv.es/handle/10251/65816reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valénciainstname:Universitat Politècnica de València (UPV)InglésengMinisterio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 TEC2012-31145 BIOCHIP KITS BASADOS EN CELDAS BIOFOTONICAS Y PLATAFORMAS AVANZADAS DE INTERROGACION OPTICAopen accesshttp://purl.org/coar/access_right/c_abf2Reserva de todos los derechoshttp://rightsstatements.org/vocab/InC/1.0/info:eu-repo/semantics/openAccessoai:riunet.upv.es:10251/658162026-06-13T07:49:27Z
dc.title.none.fl_str_mv	Development of a versatile biotinylated material based on SU-8
title	Development of a versatile biotinylated material based on SU-8
spellingShingle	Development of a versatile biotinylated material based on SU-8 Ortega Higueruelo, Francisco José IMMOBILIZATION PHOTORESIST SURFACE MODIFICATION BIOMOLECULES MEMS ASSAYS DNA PHOTOLITHOGRAPHY MICROSTRUCTURES FABRICATION QUIMICA ANALITICA
title_short	Development of a versatile biotinylated material based on SU-8
title_full	Development of a versatile biotinylated material based on SU-8
title_fullStr	Development of a versatile biotinylated material based on SU-8
title_full_unstemmed	Development of a versatile biotinylated material based on SU-8
title_sort	Development of a versatile biotinylated material based on SU-8
dc.creator.none.fl_str_mv	Ortega Higueruelo, Francisco José Sanza, Francisco J. Laguna, Mari Fe Holgado Bolaños, Miguel Casquel Del Campo, Rafael Angulo Barrios, Carlos López Romero, David Bañuls Polo, María-José\|\|\|0000-0002-2422-7731 Maquieira, Angel\|\|\|0000-0003-4641-4957 Puchades, Rosa\|\|\|0000-0002-9329-1593
author	Ortega Higueruelo, Francisco José
author_facet	Ortega Higueruelo, Francisco José Sanza, Francisco J. Laguna, Mari Fe Holgado Bolaños, Miguel Casquel Del Campo, Rafael Angulo Barrios, Carlos López Romero, David Bañuls Polo, María-José\|\|\|0000-0002-2422-7731 Maquieira, Angel\|\|\|0000-0003-4641-4957 Puchades, Rosa\|\|\|0000-0002-9329-1593
author_role	author
author2	Sanza, Francisco J. Laguna, Mari Fe Holgado Bolaños, Miguel Casquel Del Campo, Rafael Angulo Barrios, Carlos López Romero, David Bañuls Polo, María-José\|\|\|0000-0002-2422-7731 Maquieira, Angel\|\|\|0000-0003-4641-4957 Puchades, Rosa\|\|\|0000-0002-9329-1593
author2_role	author author author author author author author author author
dc.contributor.none.fl_str_mv	Departamento de Química Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural Ministerio de Economía y Competitividad Repositorio Institucional de la Universitat Politècnica de València Riunet
dc.subject.none.fl_str_mv	IMMOBILIZATION PHOTORESIST SURFACE MODIFICATION BIOMOLECULES MEMS ASSAYS DNA PHOTOLITHOGRAPHY MICROSTRUCTURES FABRICATION QUIMICA ANALITICA
topic	IMMOBILIZATION PHOTORESIST SURFACE MODIFICATION BIOMOLECULES MEMS ASSAYS DNA PHOTOLITHOGRAPHY MICROSTRUCTURES FABRICATION QUIMICA ANALITICA
description	[EN] The negative epoxy-based SU-8 photoresist has a wide variety of applications within the semiconductor industry, photonics and lab-on-a-chip devices, and it is emerging as an alternative to silicon-based devices for sensing purposes. In the present work, biotinylation of the SU-8 polymer surface promoted by light is reported. As a result, a novel, effective, and low-cost material, focusing on the immobilization of bioreceptors and consequent biosensing, is developed. This material allows the spatial discrimination depending on the irradiation of desired areas. The most salient feature is that the photobiotin may be directly incorporated into the SU-8 curing process, consequently reducing time and cost. The potential use of this substrate is demonstrated by the immunoanalytical detection of the synthetic steroid gestrinone, showing excellent performances. Moreover, the naked eye biodetection due to the transparent SU-8 substrate, and simple instrumental quantification are additional advantages.
publishDate	2013
dc.date.none.fl_str_mv	2013 2013-01-01
dc.type.none.fl_str_mv	journal article http://purl.org/coar/resource_type/c_6501 VoR http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv	info:eu-repo/semantics/article
format	article
dc.identifier.none.fl_str_mv	https://riunet.upv.es/handle/10251/65816
url	https://riunet.upv.es/handle/10251/65816
dc.language.none.fl_str_mv	Inglés eng
language_invalid_str_mv	Inglés
language	eng
dc.relation.none.fl_str_mv	Ministerio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 TEC2012-31145 BIOCHIP KITS BASADOS EN CELDAS BIOFOTONICAS Y PLATAFORMAS AVANZADAS DE INTERROGACION OPTICA
dc.rights.none.fl_str_mv	open access http://purl.org/coar/access_right/c_abf2 Reserva de todos los derechos http://rightsstatements.org/vocab/InC/1.0/
dc.rights.openaire.fl_str_mv	info:eu-repo/semantics/openAccess
rights_invalid_str_mv	open access http://purl.org/coar/access_right/c_abf2 Reserva de todos los derechos http://rightsstatements.org/vocab/InC/1.0/
eu_rights_str_mv	openAccess
dc.format.none.fl_str_mv	application/pdf application/pdf
dc.publisher.none.fl_str_mv	Royal Society of Chemistry
publisher.none.fl_str_mv	Royal Society of Chemistry
dc.source.none.fl_str_mv	reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia instname:Universitat Politècnica de València (UPV)
instname_str	Universitat Politècnica de València (UPV)
reponame_str	RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
collection	RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
repository.name.fl_str_mv
repository.mail.fl_str_mv
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Development of a versatile biotinylated material based on SU-8

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