Optimization of guanosine-based hydrogels with boric acid derivatives for enhanced long-term stability and cell survival

MG-G has received funding from the postdoctoral fellowship programme Beatriu de Pinós (2018 BP 00155), funded by the Secretary of Universities and Research (Government of Catalonia) and by the Horizon 2020 programme of research and innovation of the European Union under the Marie Sklodowska-Curie gr...

Descripción completa

Detalles Bibliográficos
Autores: Merino Gómez, Maria, Godoy-Gallardo, Maria, Wendner, Mathias, Mateos Timoneda, Miguel Angel, Gil, FJ, Perez, Roman A.
Tipo de recurso: artículo
Fecha de publicación:2023
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:20.500.12328/3679
Acceso en línea:http://hdl.handle.net/20.500.12328/3679
https://dx.doi.org/10.3389/fbioe.2023.1147943
Access Level:acceso abierto
Palabra clave:Hidrogels a base de guanosina
Derivats de l'àcid bòric
Nucleòsids
Impressió 3D
Imprimible hidrogels
Hidrogeles a base de guanosina
Derivados del ácido bórico
Nucleósido
Impresión 3D
Imprimible hidrogeles
Guanosine-based hydrogels
Boric acid derivatives
Nucleoside
3D printing
Printable hydrogels
616.3
id ES_33d3fecbf6d6472a3d1d71405ba0657a
oai_identifier_str oai:recercat.cat:20.500.12328/3679
network_acronym_str ES
network_name_str España
repository_id_str
dc.title.none.fl_str_mv Optimization of guanosine-based hydrogels with boric acid derivatives for enhanced long-term stability and cell survival
title Optimization of guanosine-based hydrogels with boric acid derivatives for enhanced long-term stability and cell survival
spellingShingle Optimization of guanosine-based hydrogels with boric acid derivatives for enhanced long-term stability and cell survival
Merino Gómez, Maria
Hidrogels a base de guanosina
Derivats de l'àcid bòric
Nucleòsids
Impressió 3D
Imprimible hidrogels
Hidrogeles a base de guanosina
Derivados del ácido bórico
Nucleósido
Impresión 3D
Imprimible hidrogeles
Guanosine-based hydrogels
Boric acid derivatives
Nucleoside
3D printing
Printable hydrogels
616.3
title_short Optimization of guanosine-based hydrogels with boric acid derivatives for enhanced long-term stability and cell survival
title_full Optimization of guanosine-based hydrogels with boric acid derivatives for enhanced long-term stability and cell survival
title_fullStr Optimization of guanosine-based hydrogels with boric acid derivatives for enhanced long-term stability and cell survival
title_full_unstemmed Optimization of guanosine-based hydrogels with boric acid derivatives for enhanced long-term stability and cell survival
title_sort Optimization of guanosine-based hydrogels with boric acid derivatives for enhanced long-term stability and cell survival
dc.creator.none.fl_str_mv Merino Gómez, Maria
Godoy-Gallardo, Maria
Wendner, Mathias
Mateos Timoneda, Miguel Angel
Gil, FJ
Perez, Roman A.
author Merino Gómez, Maria
author_facet Merino Gómez, Maria
Godoy-Gallardo, Maria
Wendner, Mathias
Mateos Timoneda, Miguel Angel
Gil, FJ
Perez, Roman A.
author_role author
author2 Godoy-Gallardo, Maria
Wendner, Mathias
Mateos Timoneda, Miguel Angel
Gil, FJ
Perez, Roman A.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Hidrogels a base de guanosina
Derivats de l'àcid bòric
Nucleòsids
Impressió 3D
Imprimible hidrogels
Hidrogeles a base de guanosina
Derivados del ácido bórico
Nucleósido
Impresión 3D
Imprimible hidrogeles
Guanosine-based hydrogels
Boric acid derivatives
Nucleoside
3D printing
Printable hydrogels
616.3
topic Hidrogels a base de guanosina
Derivats de l'àcid bòric
Nucleòsids
Impressió 3D
Imprimible hidrogels
Hidrogeles a base de guanosina
Derivados del ácido bórico
Nucleósido
Impresión 3D
Imprimible hidrogeles
Guanosine-based hydrogels
Boric acid derivatives
Nucleoside
3D printing
Printable hydrogels
616.3
description MG-G has received funding from the postdoctoral fellowship programme Beatriu de Pinós (2018 BP 00155), funded by the Secretary of Universities and Research (Government of Catalonia) and by the Horizon 2020 programme of research and innovation of the European Union under the Marie Sklodowska-Curie grant agreement No. 801370. MM-T is supported by the Spanish Ministry of Science with the project (RTI2018-096320-B-C21, 2018). RP is supported by the Spanish Ministry by the Ramón y Cajal Program (RYC2018-025977-I) and MINECO/FEDER project (RTI2018-096088-J-100). Additional financial support was provided by the Government of Catalonia (2017 SGR 708).
publishDate 2023
dc.date.none.fl_str_mv 2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12328/3679
https://dx.doi.org/10.3389/fbioe.2023.1147943
url http://hdl.handle.net/20.500.12328/3679
https://dx.doi.org/10.3389/fbioe.2023.1147943
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Frontiers in Bioengineering and Biotechnology
11
https://www.frontiersin.org/articles/10.3389/fbioe.2023.1147943/full
info:eu-repo/grantAgreement/ES/MICINN/RTI2018-096320-B-C21
info:eu-repo/grantAgreement/ES/MINECO/RTI2018-096088-J-100
dc.rights.none.fl_str_mv https://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 16
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869405771940233216
spelling Optimization of guanosine-based hydrogels with boric acid derivatives for enhanced long-term stability and cell survivalMerino Gómez, MariaGodoy-Gallardo, MariaWendner, MathiasMateos Timoneda, Miguel AngelGil, FJPerez, Roman A.Hidrogels a base de guanosinaDerivats de l'àcid bòricNucleòsidsImpressió 3DImprimible hidrogelsHidrogeles a base de guanosinaDerivados del ácido bóricoNucleósidoImpresión 3DImprimible hidrogelesGuanosine-based hydrogelsBoric acid derivativesNucleoside3D printingPrintable hydrogels616.3MG-G has received funding from the postdoctoral fellowship programme Beatriu de Pinós (2018 BP 00155), funded by the Secretary of Universities and Research (Government of Catalonia) and by the Horizon 2020 programme of research and innovation of the European Union under the Marie Sklodowska-Curie grant agreement No. 801370. MM-T is supported by the Spanish Ministry of Science with the project (RTI2018-096320-B-C21, 2018). RP is supported by the Spanish Ministry by the Ramón y Cajal Program (RYC2018-025977-I) and MINECO/FEDER project (RTI2018-096088-J-100). Additional financial support was provided by the Government of Catalonia (2017 SGR 708).Tissue defects can lead to serious health problems and often require grafts or transplants to repair damaged soft tissues. However, these procedures can be complex and may not always be feasible due to a lack of available tissue. Hydrogels have shown potential as a replacement for tissue grafts due to their ability to support cell survival and encapsulate biomolecules such as growth factors. In particular, guanosine-based hydrogels have been explored as a potential solution, but they often exhibit limited stability which hampers their use in the biofabrication of complex grafts. To address this issue, we explored the use of borate ester chemistry and more complex boric acid derivatives to improve the stability and properties of guanosine-based hydrogels. We hypothesized that the aromatic rings in these derivatives would enhance the stability and printability of the hydrogels through added π-π stack interactions. After optimization, 13 compositions containing either 2-naphthylboronic acid or boric acid were selected. Morphology studies shows a well-defined nanofibrilar structure with good printable properties (thixotropic behaviour, print fidelity and printability). Moreover, the pH of all tested hydrogels was within the range suitable for cell viability (7.4–8.3). Nevertheless, only the boric acid-based formulations were stable for at least 7 days. Thus, our results clearly demonstrated that the presence of additional aromatic rings did actually impair the hydrogel properties. We speculate that this is due to steric hindrance caused by adjacent groups, which disrupt the correct orientation of the aromatic groups required for effective π-π stack interactions of the guanosine building block. Despite this drawback, the developed guanosine-boric acid hydrogel exhibited good thixotropic properties and was able to support cell survival, proliferation, and migration. For instance, SaOS-2 cells planted on these printed structures readily migrated into the hydrogel and showed nearly 100% cell viability after 7 days. In conclusion, our findings highlight the potential of guanosine-boric acid hydrogels as tissue engineering scaffolds that can be readily enhanced with living cells and bioactive molecules. Thus, our work represents a significant advancement towards the development of functionalized guanosine-based hydrogels.info:eu-repo/semantics/publishedVersionFrontiers Media2023info:eu-repo/semantics/article16http://hdl.handle.net/20.500.12328/3679https://dx.doi.org/10.3389/fbioe.2023.1147943reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésFrontiers in Bioengineering and Biotechnology11https://www.frontiersin.org/articles/10.3389/fbioe.2023.1147943/fullinfo:eu-repo/grantAgreement/ES/MICINN/RTI2018-096320-B-C21info:eu-repo/grantAgreement/ES/MINECO/RTI2018-096088-J-100© 2023 Merino-Gómez, Godoy-Gallardo, Wendner, Mateos-Timoneda, Gil and Perez. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:20.500.12328/36792026-05-29T05:05:01Z
score 15,81155

Similares en LA Referencia