Directed evolution of extremophilic laccases towards industrial lignin valorisation

This effort has culminated in the development of a patent (EP21382978, PCT/EP2022/080219), showcasing the potential of the tailor-made extremophilic HRPLs for their application in wood conversion into bleached kraft pulp and wood fibreboards as well as for kraft lignin valorisation.

Bibliographic Details
Author: Rodríguez-Escribano, David
Format: doctoral thesis
Publication Date:2024
Country:España
Institution:Consejo Superior de Investigaciones Científicas (CSIC)
Repository:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/390069
Online Access:http://hdl.handle.net/10261/390069
Access Level:Open access
Keyword:Extremophilic laccases
Industrial lignin
Lacasas extremófilas
Ligninas industriales
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spelling Directed evolution of extremophilic laccases towards industrial lignin valorisationEvolución dirigida de lacasas fúngicas extremófilas para la valoración de ligninas industrialesRodríguez-Escribano, DavidExtremophilic laccasesIndustrial ligninLacasas extremófilasLigninas industrialesThis effort has culminated in the development of a patent (EP21382978, PCT/EP2022/080219), showcasing the potential of the tailor-made extremophilic HRPLs for their application in wood conversion into bleached kraft pulp and wood fibreboards as well as for kraft lignin valorisation.Tesis inédita de la Universidad Complutense de Madrid, Facultad de Farmacia, leída el 05/07/2024.--Programa de Doctorado en Microbiología y Parasitología.--191 pagIn the quest for sustainable industrial solutions, lignocellulose emerges as an essential renewable feedstock for biorefineries. Lignin represents a unique opportunity as a raw material due to its aromatic chemical composition and the abundance of leftover lignins from the pulp and paper industry (particularly from kraft pulp mills) that are currently underutilised. However, lignin's structural heterogeneity and recalcitrance to depolymerisation remain a major obstacle to its exploitation, and innovative approaches to its valorisation are needed. In this context, ligninolytic oxidoreductases such as laccases, secreted by basidiomycete fungi responsible for wood decay in nature, have been identified as potential green biocatalysts for lignin valorisation. Nonetheless, wild laccases work at acidic pH and mild temperatures, conditions that are often far from the indusrrial operation conditions. Fortunately, directed evolution makes it possible to confer new functionalities to wild-type enzymes and adapt their properties to the desired application conditions...La lignina es una materia prima renovable vital para las biorrefinerías, que son de gran relevancia en la búsqueda de soluciones industriales sostenibles, debido a su composición aromática y a la gran cantidad de lignina sobrante de la industria de la pasta y el papel (sobre todo de las fábricas de pasta kraft). Sin embargo, la heterogeneidad estructural de la lignina y su recalcitrancia a la despolimerización siguen siendo un obstáculo importante para su explotación, por lo que se necesitan enfoques innovadores para poder valorizarla. En este contexto, las oxidorreductasas ligninolíticas como las lacasas, secretadas por hongos basidiomicetos responsables de la descomposición de la madera en la naturaleza, se han identificado como potenciales biocatalizadores sostenibles para la valorización de la lignina. No obstante, las lacasas naturales funcionan a pH ácido y temperatura ambiente, condiciones que a menudo distan mucho de las exigentes condiciones industriales. Afortunadamente, la evolución dirigida permite conferir nuevas funcionalidades a las enzimas y adaptar sus propiedades a las condiciones de aplicación deseadas...Peer reviewedUniversidad Complutense de MadridCSIC - Centro de Investigaciones Biológicas Margarita Salas (CIB)Camarero, SusanaRodríguez-Escribano, David [0000-0002-3803-1182]Camarero, Susana [0000-0002-2812-895X]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202520252024info:eu-repo/semantics/doctoralThesishttp://purl.org/coar/resource_type/c_db06application/pdfhttp://hdl.handle.net/10261/390069reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://hdl.handle.net/20.500.14352/117952Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3900692026-05-22T06:33:51Z
dc.title.none.fl_str_mv Directed evolution of extremophilic laccases towards industrial lignin valorisation
Evolución dirigida de lacasas fúngicas extremófilas para la valoración de ligninas industriales
title Directed evolution of extremophilic laccases towards industrial lignin valorisation
spellingShingle Directed evolution of extremophilic laccases towards industrial lignin valorisation
Rodríguez-Escribano, David
Extremophilic laccases
Industrial lignin
Lacasas extremófilas
Ligninas industriales
title_short Directed evolution of extremophilic laccases towards industrial lignin valorisation
title_full Directed evolution of extremophilic laccases towards industrial lignin valorisation
title_fullStr Directed evolution of extremophilic laccases towards industrial lignin valorisation
title_full_unstemmed Directed evolution of extremophilic laccases towards industrial lignin valorisation
title_sort Directed evolution of extremophilic laccases towards industrial lignin valorisation
dc.creator.none.fl_str_mv Rodríguez-Escribano, David
author Rodríguez-Escribano, David
author_facet Rodríguez-Escribano, David
author_role author
dc.contributor.none.fl_str_mv Camarero, Susana
Rodríguez-Escribano, David [0000-0002-3803-1182]
Camarero, Susana [0000-0002-2812-895X]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Extremophilic laccases
Industrial lignin
Lacasas extremófilas
Ligninas industriales
topic Extremophilic laccases
Industrial lignin
Lacasas extremófilas
Ligninas industriales
description This effort has culminated in the development of a patent (EP21382978, PCT/EP2022/080219), showcasing the potential of the tailor-made extremophilic HRPLs for their application in wood conversion into bleached kraft pulp and wood fibreboards as well as for kraft lignin valorisation.
publishDate 2024
dc.date.none.fl_str_mv 2024
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/doctoralThesis
http://purl.org/coar/resource_type/c_db06
format doctoralThesis
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/390069
url http://hdl.handle.net/10261/390069
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://hdl.handle.net/20.500.14352/117952
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidad Complutense de Madrid
CSIC - Centro de Investigaciones Biológicas Margarita Salas (CIB)
publisher.none.fl_str_mv Universidad Complutense de Madrid
CSIC - Centro de Investigaciones Biológicas Margarita Salas (CIB)
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.811543

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