Whole-Genome Transcriptomics of PBMC to Identify Biomarkers of Early Metabolic Risk in Apparently Healthy People with Overweight-Obesity and in Normal-Weight Subjects

Peripheral blood mononuclear cells (PBMC) provide a useful and minimally invasive source of biomarkers. Here to identify PBMC transcriptomic biomarkers predictive of metabolic impairment related to increased adiposity is aimed. The study analyzed the global PBMC transcriptome in metabolically health...

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Autores: Costa, Andrea, van der Stelt, Inge, Reynés, Bàrbara, Konieczna, Jadwiga, Fiol Sala, Miquel, Keijer, Jaap, Palou, Andreu, Romaguera, Dora, van Schothorst, Evert M., Oliver, Paula
Tipo de recurso: artículo
Fecha de publicación:2023
País:España
Institución:Conselleria de Salut i Consum del Govern de les Illes Balears
Repositorio:Docusalut
Idioma:inglés
OAI Identifier:oai:docusalut.com:20.500.13003/18827
Acceso en línea:https://hdl.handle.net/20.500.13003/18827
Access Level:acceso abierto
Palabra clave:Transcriptome
Biomarkers
Gene Expression Profiling
Humans
Body Mass Index
Overweight
Leukocytes, Mononuclear
Obesity
Índice de Masa Corporal
Humanos
Obesidad
Leucocitos Mononucleares
Biomarcadores
Perfilación de la Expresión Génica
Sobrepeso
Transcriptoma
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spelling Whole-Genome Transcriptomics of PBMC to Identify Biomarkers of Early Metabolic Risk in Apparently Healthy People with Overweight-Obesity and in Normal-Weight SubjectsCosta, Andreavan der Stelt, IngeReynés, BàrbaraKonieczna, JadwigaFiol Sala, MiquelKeijer, JaapPalou, AndreuRomaguera, Doravan Schothorst, Evert M.Oliver, PaulaTranscriptomeBiomarkersGene Expression ProfilingHumansBody Mass IndexOverweightLeukocytes, MononuclearObesityÍndice de Masa CorporalHumanosObesidadLeucocitos MononuclearesBiomarcadoresPerfilación de la Expresión GénicaSobrepesoTranscriptomaPeripheral blood mononuclear cells (PBMC) provide a useful and minimally invasive source of biomarkers. Here to identify PBMC transcriptomic biomarkers predictive of metabolic impairment related to increased adiposity is aimed. The study analyzed the global PBMC transcriptome in metabolically healthy (normoglycemic) volunteers with overweight-obesity (OW-OB, n = 12), and in subjects with metabolically obese normal-weight (MONW, n = 5) phenotype, in comparison to normal-weight (NW, n = 12) controls. The study identifies 1072 differentially expressed genes (DEGs) in OW-OB versus NW and 992 in MONW versus NW. Hierarchical clustering of the top 100 DEGs clearly distinguishes OW-OB and MONW from NW. Remarkably, the OW-OB and MONW phenotypes share 257 DEGs regulated in the same direction. The top up-regulated gene CXCL8, coding for interleukin 8, with a role in obesity-related pathologies, is of special interest as a potential marker for predicting increased metabolic risk. CXCL8 expression is increased mainly in the MONW group and correlated directly with C-reactive protein levels. PBMC gene expression analysis of CXCL8 or a pool of DEGs may be used to identify early metabolic risk in an apparently healthy population regardless of their BMI, i.e., subjects with OW-OB or MONW phenotype and to apply adequate and personalized nutritional preventive strategies.Wiley20232023-02-0120232023-02-01research articlehttp://purl.org/coar/resource_type/c_2df8fbb1info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.13003/18827reponame:Docusalutinstname:Conselleria de Salut i Consum del Govern de les Illes BalearsInglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:docusalut.com:20.500.13003/188272026-06-22T12:44:07Z
dc.title.none.fl_str_mv Whole-Genome Transcriptomics of PBMC to Identify Biomarkers of Early Metabolic Risk in Apparently Healthy People with Overweight-Obesity and in Normal-Weight Subjects
title Whole-Genome Transcriptomics of PBMC to Identify Biomarkers of Early Metabolic Risk in Apparently Healthy People with Overweight-Obesity and in Normal-Weight Subjects
spellingShingle Whole-Genome Transcriptomics of PBMC to Identify Biomarkers of Early Metabolic Risk in Apparently Healthy People with Overweight-Obesity and in Normal-Weight Subjects
Costa, Andrea
Transcriptome
Biomarkers
Gene Expression Profiling
Humans
Body Mass Index
Overweight
Leukocytes, Mononuclear
Obesity
Índice de Masa Corporal
Humanos
Obesidad
Leucocitos Mononucleares
Biomarcadores
Perfilación de la Expresión Génica
Sobrepeso
Transcriptoma
title_short Whole-Genome Transcriptomics of PBMC to Identify Biomarkers of Early Metabolic Risk in Apparently Healthy People with Overweight-Obesity and in Normal-Weight Subjects
title_full Whole-Genome Transcriptomics of PBMC to Identify Biomarkers of Early Metabolic Risk in Apparently Healthy People with Overweight-Obesity and in Normal-Weight Subjects
title_fullStr Whole-Genome Transcriptomics of PBMC to Identify Biomarkers of Early Metabolic Risk in Apparently Healthy People with Overweight-Obesity and in Normal-Weight Subjects
title_full_unstemmed Whole-Genome Transcriptomics of PBMC to Identify Biomarkers of Early Metabolic Risk in Apparently Healthy People with Overweight-Obesity and in Normal-Weight Subjects
title_sort Whole-Genome Transcriptomics of PBMC to Identify Biomarkers of Early Metabolic Risk in Apparently Healthy People with Overweight-Obesity and in Normal-Weight Subjects
dc.creator.none.fl_str_mv Costa, Andrea
van der Stelt, Inge
Reynés, Bàrbara
Konieczna, Jadwiga
Fiol Sala, Miquel
Keijer, Jaap
Palou, Andreu
Romaguera, Dora
van Schothorst, Evert M.
Oliver, Paula
author Costa, Andrea
author_facet Costa, Andrea
van der Stelt, Inge
Reynés, Bàrbara
Konieczna, Jadwiga
Fiol Sala, Miquel
Keijer, Jaap
Palou, Andreu
Romaguera, Dora
van Schothorst, Evert M.
Oliver, Paula
author_role author
author2 van der Stelt, Inge
Reynés, Bàrbara
Konieczna, Jadwiga
Fiol Sala, Miquel
Keijer, Jaap
Palou, Andreu
Romaguera, Dora
van Schothorst, Evert M.
Oliver, Paula
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv
dc.subject.none.fl_str_mv Transcriptome
Biomarkers
Gene Expression Profiling
Humans
Body Mass Index
Overweight
Leukocytes, Mononuclear
Obesity
Índice de Masa Corporal
Humanos
Obesidad
Leucocitos Mononucleares
Biomarcadores
Perfilación de la Expresión Génica
Sobrepeso
Transcriptoma
topic Transcriptome
Biomarkers
Gene Expression Profiling
Humans
Body Mass Index
Overweight
Leukocytes, Mononuclear
Obesity
Índice de Masa Corporal
Humanos
Obesidad
Leucocitos Mononucleares
Biomarcadores
Perfilación de la Expresión Génica
Sobrepeso
Transcriptoma
description Peripheral blood mononuclear cells (PBMC) provide a useful and minimally invasive source of biomarkers. Here to identify PBMC transcriptomic biomarkers predictive of metabolic impairment related to increased adiposity is aimed. The study analyzed the global PBMC transcriptome in metabolically healthy (normoglycemic) volunteers with overweight-obesity (OW-OB, n = 12), and in subjects with metabolically obese normal-weight (MONW, n = 5) phenotype, in comparison to normal-weight (NW, n = 12) controls. The study identifies 1072 differentially expressed genes (DEGs) in OW-OB versus NW and 992 in MONW versus NW. Hierarchical clustering of the top 100 DEGs clearly distinguishes OW-OB and MONW from NW. Remarkably, the OW-OB and MONW phenotypes share 257 DEGs regulated in the same direction. The top up-regulated gene CXCL8, coding for interleukin 8, with a role in obesity-related pathologies, is of special interest as a potential marker for predicting increased metabolic risk. CXCL8 expression is increased mainly in the MONW group and correlated directly with C-reactive protein levels. PBMC gene expression analysis of CXCL8 or a pool of DEGs may be used to identify early metabolic risk in an apparently healthy population regardless of their BMI, i.e., subjects with OW-OB or MONW phenotype and to apply adequate and personalized nutritional preventive strategies.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023-02-01
2023
2023-02-01
dc.type.none.fl_str_mv research article
http://purl.org/coar/resource_type/c_2df8fbb1
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.13003/18827
url https://hdl.handle.net/20.500.13003/18827
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:Docusalut
instname:Conselleria de Salut i Consum del Govern de les Illes Balears
instname_str Conselleria de Salut i Consum del Govern de les Illes Balears
reponame_str Docusalut
collection Docusalut
repository.name.fl_str_mv
repository.mail.fl_str_mv
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