Shaping mammalian tissues with light : optogenetic control of apical constriction

During embryonic development, cellular forces synchronize in space and time to generate functional tissue shapes. Apical constriction is a force-generating process necessary to provoke the folding of multiple organ primordia during early development. In this thesis, we used a synthetic biology appro...

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Detalles Bibliográficos
Autor: Martínez Ara, Guillermo
Tipo de recurso: tesis doctoral
Estado:Versión publicada
Fecha de publicación:2022
País:España
Institución:CBUC, CESCA
Repositorio:TDR. Tesis Doctorales en Red
OAI Identifier:oai:www.tdx.cat:10803/675511
Acceso en línea:http://hdl.handle.net/10803/675511
Access Level:acceso abierto
Palabra clave:Morphogenesis
Optogenetics
Epithelial
Synthetic
Development
Morfogenesis
Optogenética
Epitelial
Sintética
Desarrollo
576
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spelling Shaping mammalian tissues with light : optogenetic control of apical constrictionMartínez Ara, GuillermoMorphogenesisOptogeneticsEpithelialSyntheticDevelopmentMorfogenesisOptogenéticaEpitelialSintéticaDesarrollo576During embryonic development, cellular forces synchronize in space and time to generate functional tissue shapes. Apical constriction is a force-generating process necessary to provoke the folding of multiple organ primordia during early development. In this thesis, we used a synthetic biology approach to engineer control over apical constriction, manipulate morphogenesis and better understand it. We developed “OptoShroom3”, a new optogenetic tool that achieves fast spatiotemporal control of apical constriction in mammalian epithelia. We used OptoShroom3 to manipulate 3D tissue shapes, using light to provoke tissue folding and alter the shape of neural organoids. We further studied the folding of epithelial colonies with 2 different proteins, Shroom3 and Lulu2, and observed that shape biases the direction of folding. Our work pioneers in the use of biological forces to manipulate shape in mammalian tissues. We expect that spatiotemporal control of morphogenesis will promote the study of feedbacks between shape, function and fate.Durante el desarrollo embrionario, las fuerzas celulares se sincronizan para generar tejidos con estructura funcional. La constricción apical es un proceso que provoca el plegamiento de órganos primitivos durante el desarrollo temprano. En esta tesis, hemos usado la biología sintética para controlar la constricción apical, manipular la morfogénesis y entenderla mejor. Hemos desarrollado “OptoShroom3”, un sistema optogenético que permite un rápido control espaciotemporal de la constricción apical en epitelios mamíferos. Hemos usado OptoShroom3 para manipular tejidos 3D, usando la luz para provocar plegamientos y alterar la forma de organoides neurales. Hemos estudiado el plegado de los tejidos epiteliales con dos proteínas diferentes (Shroom3 y Lulu2), observando que la forma original condiciona la dirección del plegado. Este trabajo impulsa el uso de fuerzas biológicas para manipular la forma de los tejidos. Esperamos que el control espaciotemporal de la morfogénesis promueva el estudio de las interacciones entre forma, función y diferenciación.Programa de doctorat en BiomedicinaUniversitat Pompeu FabraEbisuya, MikiUniversitat Pompeu Fabra. Departament de Medicina i Ciències de la Vida202220242022info:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/publishedVersion116 p.application/pdfapplication/pdfhttp://hdl.handle.net/10803/675511TDX (Tesis Doctorals en Xarxa)reponame:TDR. Tesis Doctorales en Redinstname:CBUC, CESCAInglésL'accés als continguts d'aquesta tesi queda condicionat a l'acceptació de les condicions d'ús establertes per la següent llicència Creative Commons: http://creativecommons.org/licenses/by/4.0/http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:www.tdx.cat:10803/6755112026-06-14T12:46:07Z
dc.title.none.fl_str_mv Shaping mammalian tissues with light : optogenetic control of apical constriction
title Shaping mammalian tissues with light : optogenetic control of apical constriction
spellingShingle Shaping mammalian tissues with light : optogenetic control of apical constriction
Martínez Ara, Guillermo
Morphogenesis
Optogenetics
Epithelial
Synthetic
Development
Morfogenesis
Optogenética
Epitelial
Sintética
Desarrollo
576
title_short Shaping mammalian tissues with light : optogenetic control of apical constriction
title_full Shaping mammalian tissues with light : optogenetic control of apical constriction
title_fullStr Shaping mammalian tissues with light : optogenetic control of apical constriction
title_full_unstemmed Shaping mammalian tissues with light : optogenetic control of apical constriction
title_sort Shaping mammalian tissues with light : optogenetic control of apical constriction
dc.creator.none.fl_str_mv Martínez Ara, Guillermo
author Martínez Ara, Guillermo
author_facet Martínez Ara, Guillermo
author_role author
dc.contributor.none.fl_str_mv Ebisuya, Miki
Universitat Pompeu Fabra. Departament de Medicina i Ciències de la Vida
dc.subject.none.fl_str_mv Morphogenesis
Optogenetics
Epithelial
Synthetic
Development
Morfogenesis
Optogenética
Epitelial
Sintética
Desarrollo
576
topic Morphogenesis
Optogenetics
Epithelial
Synthetic
Development
Morfogenesis
Optogenética
Epitelial
Sintética
Desarrollo
576
description During embryonic development, cellular forces synchronize in space and time to generate functional tissue shapes. Apical constriction is a force-generating process necessary to provoke the folding of multiple organ primordia during early development. In this thesis, we used a synthetic biology approach to engineer control over apical constriction, manipulate morphogenesis and better understand it. We developed “OptoShroom3”, a new optogenetic tool that achieves fast spatiotemporal control of apical constriction in mammalian epithelia. We used OptoShroom3 to manipulate 3D tissue shapes, using light to provoke tissue folding and alter the shape of neural organoids. We further studied the folding of epithelial colonies with 2 different proteins, Shroom3 and Lulu2, and observed that shape biases the direction of folding. Our work pioneers in the use of biological forces to manipulate shape in mammalian tissues. We expect that spatiotemporal control of morphogenesis will promote the study of feedbacks between shape, function and fate.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/doctoralThesis
info:eu-repo/semantics/publishedVersion
format doctoralThesis
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10803/675511
url http://hdl.handle.net/10803/675511
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 116 p.
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Universitat Pompeu Fabra
publisher.none.fl_str_mv Universitat Pompeu Fabra
dc.source.none.fl_str_mv TDX (Tesis Doctorals en Xarxa)
reponame:TDR. Tesis Doctorales en Red
instname:CBUC, CESCA
instname_str CBUC, CESCA
reponame_str TDR. Tesis Doctorales en Red
collection TDR. Tesis Doctorales en Red
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,301603

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