Rgf1p (Rho1p GEF) is required for double-strand break repair in fission yeast

Datos de investigación en: http://hdl.handle.net/10366/146281

Bibliographic Details
Authors: Manjón Pérez, Elvira, Edreira González, Tomás, Muñoz Félix, Sofía, Sánchez Martín, Yolanda
Format: article
Status:Published version
Publication Date:2017
Country:España
Institution:Universidad de Salamanca (USAL)
Repository:GREDOS. Repositorio Institucional de la Universidad de Salamanca
OAI Identifier:oai:gredos.usal.es:10366/146281
Online Access:http://hdl.handle.net/10366/146281
Access Level:Open access
Keyword:Fission Yeast
DNA damage
Rho1p GEF
Molecular Biology
DNA Damage
2414.10 Micología (Levaduras)
2415.01 Biología Molecular de Microorganismos
daño del ADN
biología molecular
id ES_2f64fb14bbd1d554f45873ab692e54a1
oai_identifier_str oai:gredos.usal.es:10366/146281
network_acronym_str ES
network_name_str España
repository_id_str
spelling Rgf1p (Rho1p GEF) is required for double-strand break repair in fission yeastManjón Pérez, ElviraEdreira González, TomásMuñoz Félix, SofíaSánchez Martín, YolandaFission YeastDNA damageRho1p GEFMolecular BiologyDNA Damage2414.10 Micología (Levaduras)2415.01 Biología Molecular de Microorganismosdaño del ADNbiología molecularDatos de investigación en: http://hdl.handle.net/10366/146281[EN]Rho GTPases are conserved molecules that control cytoskeletal dynamics. These functions are expedited by Rho GEFs that stimulate the release of GDP to enable GTP binding, thereby allowing Rho proteins to initiate intracellular signaling. How Rho GEFs and Rho GTPases protect cells from DNA damage is unknown. Here, we explore the extreme sensitivity of a deletion mutation in the Rho1p exchange factor Rgf1p to the DNA break/inducing antibiotic phleomycin (Phl). The Rgf1p mutant cells are defective in reentry into the cell cycle following the induction of severe DNA damage. This phenotype correlates with the inability of rgf1Δ cells to efficiently repair fragmented chromosomes after Phl treatment. Consistent with this observation Rad11p (ssDNA binding protein, RPA), Rad52p, Rad54p and Rad51p, which facilitate strand invasion in the process of homology-directed repair (HDR), are permanently stacked in Phl-induced foci in rgf1Δ cells. These phenotypes are phenocopied by genetic inhibition of Rho1p. Our data provide evidence that Rgf1p/Rho1p activity positively controls a repair function that confers resistance against the anti-cancer drug Phl.CICYT, Spain [BFU2011-24683/BMC]; Junta de Castilla y León [SA073U14]. Funding for open access charge: MINECO.Oxford University Press202120212017info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10366/146281reponame:GREDOS. Repositorio Institucional de la Universidad de Salamancainstname:Universidad de Salamanca (USAL)Ingléshttp://hdl.handle.net/10366/146281Attribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:gredos.usal.es:10366/1462812026-06-07T06:28:51Z
dc.title.none.fl_str_mv Rgf1p (Rho1p GEF) is required for double-strand break repair in fission yeast
title Rgf1p (Rho1p GEF) is required for double-strand break repair in fission yeast
spellingShingle Rgf1p (Rho1p GEF) is required for double-strand break repair in fission yeast
Manjón Pérez, Elvira
Fission Yeast
DNA damage
Rho1p GEF
Molecular Biology
DNA Damage
2414.10 Micología (Levaduras)
2415.01 Biología Molecular de Microorganismos
daño del ADN
biología molecular
title_short Rgf1p (Rho1p GEF) is required for double-strand break repair in fission yeast
title_full Rgf1p (Rho1p GEF) is required for double-strand break repair in fission yeast
title_fullStr Rgf1p (Rho1p GEF) is required for double-strand break repair in fission yeast
title_full_unstemmed Rgf1p (Rho1p GEF) is required for double-strand break repair in fission yeast
title_sort Rgf1p (Rho1p GEF) is required for double-strand break repair in fission yeast
dc.creator.none.fl_str_mv Manjón Pérez, Elvira
Edreira González, Tomás
Muñoz Félix, Sofía
Sánchez Martín, Yolanda
author Manjón Pérez, Elvira
author_facet Manjón Pérez, Elvira
Edreira González, Tomás
Muñoz Félix, Sofía
Sánchez Martín, Yolanda
author_role author
author2 Edreira González, Tomás
Muñoz Félix, Sofía
Sánchez Martín, Yolanda
author2_role author
author
author
dc.subject.none.fl_str_mv Fission Yeast
DNA damage
Rho1p GEF
Molecular Biology
DNA Damage
2414.10 Micología (Levaduras)
2415.01 Biología Molecular de Microorganismos
daño del ADN
biología molecular
topic Fission Yeast
DNA damage
Rho1p GEF
Molecular Biology
DNA Damage
2414.10 Micología (Levaduras)
2415.01 Biología Molecular de Microorganismos
daño del ADN
biología molecular
description Datos de investigación en: http://hdl.handle.net/10366/146281
publishDate 2017
dc.date.none.fl_str_mv 2017
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10366/146281
url http://hdl.handle.net/10366/146281
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://hdl.handle.net/10366/146281
dc.rights.none.fl_str_mv Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:GREDOS. Repositorio Institucional de la Universidad de Salamanca
instname:Universidad de Salamanca (USAL)
instname_str Universidad de Salamanca (USAL)
reponame_str GREDOS. Repositorio Institucional de la Universidad de Salamanca
collection GREDOS. Repositorio Institucional de la Universidad de Salamanca
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869405474981412864
score 15,301603

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