In vitro antiviral properties of two recombinant sendai virus vectors encoding ORFV 011 and ORFV 059 genes

Orf virus (ORFV) is a globally distributed zoonotic parapoxvirus that causes a highly contagious mucocutaneous disease in small ruminants. Despite the urgent demand for vaccination-based control, no licensed vaccines are currently available universally. In this study, we generated two recombinant Se...

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Autores: Gómez, Álex, Glaría Ezquer, Idoia, Moncayola, Irati, Puzol, Leonor, Arriazu, Laura, Calero, Ainhoa, Blas, Ignacio de, Nazábal, Mikel, Hualde, Itziar, Lee, Benhur, Luján, Lluís, Amann, Ralf, Echeverría Garín, Irache, Reina Arias, Ramsés
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2026
País:España
Institución:Universidad San Jorge (USJ)
Repositorio:Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
OAI Identifier:oai:dnet:academicae__::4693e8c4b7dbc055643aa6a50f8d41dc
Acceso en línea:https://hdl.handle.net/2454/56914
Access Level:acceso abierto
Palabra clave:Orf virus
Sendai virus
Viral vector
Innate immunity
Antiviral
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spelling In vitro antiviral properties of two recombinant sendai virus vectors encoding ORFV 011 and ORFV 059 genesGómez, ÁlexGlaría Ezquer, IdoiaMoncayola, IratiPuzol, LeonorArriazu, LauraCalero, AinhoaBlas, Ignacio deNazábal, MikelHualde, ItziarLee, BenhurLuján, LluísAmann, RalfEcheverría Garín, IracheReina Arias, RamsésOrf virusSendai virusViral vectorInnate immunityAntiviralOrf virus (ORFV) is a globally distributed zoonotic parapoxvirus that causes a highly contagious mucocutaneous disease in small ruminants. Despite the urgent demand for vaccination-based control, no licensed vaccines are currently available universally. In this study, we generated two recombinant Sendai virus (SeV) vectors expressing ORFV 011 (rSeV-GFP-B2L) and ORFV 059 (rSeV-GFP-059) genes and evaluated their ability to stimulate antiviral responses in vitro. Following the transduction, we assessed transgene expression, innate immune activation, induction of interferon-stimulated genes (A3Z1, OBST2, SAMHD1), and antiviral activity. Both vectors significantly upregulated pattern recognition receptors (TLRs, RIG-I) and type I interferon (IFN-β) genes, with rSeV-GFP-059 inducing the strongest response. Remarkably, OBST2 was robustly upregulated, suggesting a potential role in restricting ORFV replication. Antiviral activity assays revealed a marked reduction in ORFV DNA copies and a mild decrease in ORFV RNA transcription in rSeVGFP-059-transduced cells, particularly at later time points, accompanied by complete abrogation of the typical cytopathic effect. Collectively, these results demonstrate that SeV-based vectors, particularly rSeV-GFP-059, efficiently prime antiviral immunity and suppress ORFV replication, establishing a promising platform for further in vivo vaccine evaluation in sheep.This research was funded by the Gobierno de Navarra, grant number PC080-081 ECTICONA and PC24-ECTIVAC-002-017-018MDPIAgronomía, Biotecnología y AlimentaciónAgronomia, Bioteknologia eta ElikaduraGobierno de Navarra / Nafarroako Gobernua2026info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/ziphttps://hdl.handle.net/2454/56914reponame:Academica-e. Repositorio Institucional de la Universidad Pública de Navarrainstname:Universidad San Jorge (USJ)Españolinfo:eu-repo/grantAgreement/Gobierno de Navarra//PC080-081-ECTICONAinfo:eu-repo/grantAgreement/Gobierno de Navarra//PC24-ECTIVAC-002-017-018© 2026 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:dnet:academicae__::4693e8c4b7dbc055643aa6a50f8d41dc2026-06-17T12:41:47Z
dc.title.none.fl_str_mv In vitro antiviral properties of two recombinant sendai virus vectors encoding ORFV 011 and ORFV 059 genes
title In vitro antiviral properties of two recombinant sendai virus vectors encoding ORFV 011 and ORFV 059 genes
spellingShingle In vitro antiviral properties of two recombinant sendai virus vectors encoding ORFV 011 and ORFV 059 genes
Gómez, Álex
Orf virus
Sendai virus
Viral vector
Innate immunity
Antiviral
title_short In vitro antiviral properties of two recombinant sendai virus vectors encoding ORFV 011 and ORFV 059 genes
title_full In vitro antiviral properties of two recombinant sendai virus vectors encoding ORFV 011 and ORFV 059 genes
title_fullStr In vitro antiviral properties of two recombinant sendai virus vectors encoding ORFV 011 and ORFV 059 genes
title_full_unstemmed In vitro antiviral properties of two recombinant sendai virus vectors encoding ORFV 011 and ORFV 059 genes
title_sort In vitro antiviral properties of two recombinant sendai virus vectors encoding ORFV 011 and ORFV 059 genes
dc.creator.none.fl_str_mv Gómez, Álex
Glaría Ezquer, Idoia
Moncayola, Irati
Puzol, Leonor
Arriazu, Laura
Calero, Ainhoa
Blas, Ignacio de
Nazábal, Mikel
Hualde, Itziar
Lee, Benhur
Luján, Lluís
Amann, Ralf
Echeverría Garín, Irache
Reina Arias, Ramsés
author Gómez, Álex
author_facet Gómez, Álex
Glaría Ezquer, Idoia
Moncayola, Irati
Puzol, Leonor
Arriazu, Laura
Calero, Ainhoa
Blas, Ignacio de
Nazábal, Mikel
Hualde, Itziar
Lee, Benhur
Luján, Lluís
Amann, Ralf
Echeverría Garín, Irache
Reina Arias, Ramsés
author_role author
author2 Glaría Ezquer, Idoia
Moncayola, Irati
Puzol, Leonor
Arriazu, Laura
Calero, Ainhoa
Blas, Ignacio de
Nazábal, Mikel
Hualde, Itziar
Lee, Benhur
Luján, Lluís
Amann, Ralf
Echeverría Garín, Irache
Reina Arias, Ramsés
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Agronomía, Biotecnología y Alimentación
Agronomia, Bioteknologia eta Elikadura
Gobierno de Navarra / Nafarroako Gobernua
dc.subject.none.fl_str_mv Orf virus
Sendai virus
Viral vector
Innate immunity
Antiviral
topic Orf virus
Sendai virus
Viral vector
Innate immunity
Antiviral
description Orf virus (ORFV) is a globally distributed zoonotic parapoxvirus that causes a highly contagious mucocutaneous disease in small ruminants. Despite the urgent demand for vaccination-based control, no licensed vaccines are currently available universally. In this study, we generated two recombinant Sendai virus (SeV) vectors expressing ORFV 011 (rSeV-GFP-B2L) and ORFV 059 (rSeV-GFP-059) genes and evaluated their ability to stimulate antiviral responses in vitro. Following the transduction, we assessed transgene expression, innate immune activation, induction of interferon-stimulated genes (A3Z1, OBST2, SAMHD1), and antiviral activity. Both vectors significantly upregulated pattern recognition receptors (TLRs, RIG-I) and type I interferon (IFN-β) genes, with rSeV-GFP-059 inducing the strongest response. Remarkably, OBST2 was robustly upregulated, suggesting a potential role in restricting ORFV replication. Antiviral activity assays revealed a marked reduction in ORFV DNA copies and a mild decrease in ORFV RNA transcription in rSeVGFP-059-transduced cells, particularly at later time points, accompanied by complete abrogation of the typical cytopathic effect. Collectively, these results demonstrate that SeV-based vectors, particularly rSeV-GFP-059, efficiently prime antiviral immunity and suppress ORFV replication, establishing a promising platform for further in vivo vaccine evaluation in sheep.
publishDate 2026
dc.date.none.fl_str_mv 2026
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2454/56914
url https://hdl.handle.net/2454/56914
dc.language.none.fl_str_mv Español
language_invalid_str_mv Español
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/Gobierno de Navarra//PC080-081-ECTICONA
info:eu-repo/grantAgreement/Gobierno de Navarra//PC24-ECTIVAC-002-017-018
dc.rights.none.fl_str_mv https://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/zip
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
instname:Universidad San Jorge (USJ)
instname_str Universidad San Jorge (USJ)
reponame_str Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
collection Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
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