Inside out porphyridium cruentum: Beyond the conventional biorefinery concept

Here, an unprecedented biorefinery approach has been designed to recover high-added value bioproducts starting from the culture ofPorphyridium cruentum. This unicellular marine red alga can secrete and accumulate high-value compounds that can find applications in a wide variety of industrial fields....

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Autores: Liberti, Davide, Imbimbo, Paola, Giustino, Enrica, D’Elia, Luigi, Ferraro, Giarita, Casillo, Angela, Illiano, Anna, Pinto, Gabriella, Di Meo, Maria Chiara, Álvarez-Rivera, Gerardo, Corsaro, Maria Michela, Amoresano, Angela, Zarrelli, Armando, Ibáñez, Elena, Merlino, Antonello, Monti, Daria Maria
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/334099
Acceso en línea:http://hdl.handle.net/10261/334099
Access Level:acceso abierto
Palabra clave:Microalgae
Cascade approach
High-added-value molecules
Phycoerythrin
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spelling Inside out porphyridium cruentum: Beyond the conventional biorefinery conceptLiberti, DavideImbimbo, PaolaGiustino, EnricaD’Elia, LuigiFerraro, GiaritaCasillo, AngelaIlliano, AnnaPinto, GabriellaDi Meo, Maria ChiaraÁlvarez-Rivera, GerardoCorsaro, Maria MichelaAmoresano, AngelaZarrelli, ArmandoIbáñez, ElenaMerlino, AntonelloMonti, Daria MariaMicroalgaeCascade approachHigh-added-value moleculesPhycoerythrinHere, an unprecedented biorefinery approach has been designed to recover high-added value bioproducts starting from the culture ofPorphyridium cruentum. This unicellular marine red alga can secrete and accumulate high-value compounds that can find applications in a wide variety of industrial fields. 300 ± 67 mg/L of exopolysaccharides were obtained from cell culture medium; phycoerythrin was efficiently extracted (40% of total extract) and isolated by single chromatography, with a purity grade that allowed the crystal structure determination at 1.60 Å; a twofold increase in β-carotene yield was obtained from the residual biomass; the final residual biomass was found to be enriched in saturated fatty acids. Thus, for the first time, a complete exploitation ofP. cruentumculture was set up.P.I. would like to acknowledge ALGAE4IBD project (FROM NATURE TO BEDSIDE-ALGAE BASED BIO COMPOUND FOR PREVENTION) funded by the European Union’s Horizon 2020 Research and Innovation program under grant agreement N° 101000501. This work was supported by the Ministry of Science and Innovation of Spain (Grant No. PID2020-113050RB-I00). G.A.-R. would like to acknowledge the Ministry of Science and Innovation (MICINN) for his “Juan de la Cierva-Incorporación” postdoctoral grant IJC2019-041482-I. A.M. and G.F. thank Elettra Synchrotron of Trieste staff for their help during X-ray diffraction data collection.Peer reviewedAmerican Chemical SocietyEuropean CommissionAgencia Estatal de Investigación (España)Ministerio de Ciencia, Innovación y Universidades (España)Elettra Sincrotrone TriesteConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232023info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/334099reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/EC/H2020/101000501info:eu-repo/grantAgreement/AEI//IJC2019-041482-Iinfo:eu-repo/grantAgreement/AEI//PID2020-113050RB-I00https://doi.org/10.1021/acssuschemeng.2c05869Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3340992026-05-22T06:33:51Z
dc.title.none.fl_str_mv Inside out porphyridium cruentum: Beyond the conventional biorefinery concept
title Inside out porphyridium cruentum: Beyond the conventional biorefinery concept
spellingShingle Inside out porphyridium cruentum: Beyond the conventional biorefinery concept
Liberti, Davide
Microalgae
Cascade approach
High-added-value molecules
Phycoerythrin
title_short Inside out porphyridium cruentum: Beyond the conventional biorefinery concept
title_full Inside out porphyridium cruentum: Beyond the conventional biorefinery concept
title_fullStr Inside out porphyridium cruentum: Beyond the conventional biorefinery concept
title_full_unstemmed Inside out porphyridium cruentum: Beyond the conventional biorefinery concept
title_sort Inside out porphyridium cruentum: Beyond the conventional biorefinery concept
dc.creator.none.fl_str_mv Liberti, Davide
Imbimbo, Paola
Giustino, Enrica
D’Elia, Luigi
Ferraro, Giarita
Casillo, Angela
Illiano, Anna
Pinto, Gabriella
Di Meo, Maria Chiara
Álvarez-Rivera, Gerardo
Corsaro, Maria Michela
Amoresano, Angela
Zarrelli, Armando
Ibáñez, Elena
Merlino, Antonello
Monti, Daria Maria
author Liberti, Davide
author_facet Liberti, Davide
Imbimbo, Paola
Giustino, Enrica
D’Elia, Luigi
Ferraro, Giarita
Casillo, Angela
Illiano, Anna
Pinto, Gabriella
Di Meo, Maria Chiara
Álvarez-Rivera, Gerardo
Corsaro, Maria Michela
Amoresano, Angela
Zarrelli, Armando
Ibáñez, Elena
Merlino, Antonello
Monti, Daria Maria
author_role author
author2 Imbimbo, Paola
Giustino, Enrica
D’Elia, Luigi
Ferraro, Giarita
Casillo, Angela
Illiano, Anna
Pinto, Gabriella
Di Meo, Maria Chiara
Álvarez-Rivera, Gerardo
Corsaro, Maria Michela
Amoresano, Angela
Zarrelli, Armando
Ibáñez, Elena
Merlino, Antonello
Monti, Daria Maria
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv European Commission
Agencia Estatal de Investigación (España)
Ministerio de Ciencia, Innovación y Universidades (España)
Elettra Sincrotrone Trieste
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Microalgae
Cascade approach
High-added-value molecules
Phycoerythrin
topic Microalgae
Cascade approach
High-added-value molecules
Phycoerythrin
description Here, an unprecedented biorefinery approach has been designed to recover high-added value bioproducts starting from the culture ofPorphyridium cruentum. This unicellular marine red alga can secrete and accumulate high-value compounds that can find applications in a wide variety of industrial fields. 300 ± 67 mg/L of exopolysaccharides were obtained from cell culture medium; phycoerythrin was efficiently extracted (40% of total extract) and isolated by single chromatography, with a purity grade that allowed the crystal structure determination at 1.60 Å; a twofold increase in β-carotene yield was obtained from the residual biomass; the final residual biomass was found to be enriched in saturated fatty acids. Thus, for the first time, a complete exploitation ofP. cruentumculture was set up.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/334099
url http://hdl.handle.net/10261/334099
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/EC/H2020/101000501
info:eu-repo/grantAgreement/AEI//IJC2019-041482-I
info:eu-repo/grantAgreement/AEI//PID2020-113050RB-I00
https://doi.org/10.1021/acssuschemeng.2c05869
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
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