VIM-2-producing Multidrug-Resistant Pseudomonas aeruginosa ST175 Clone, Spain

A total of 183 patients were colonized or infected with multidrug-resistant Pseudomonas aeruginosa isolates at a hospital in Spain during 2007-2010; prevalence increased over this period from 2.8% to 15.3%. To characterize these isolates, we performed molecular epidemiologic and drug resistance anal...

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Detalles Bibliográficos
Autores: Viedma, Esther, Juan, Carlos, Villa, Jennifer, Barrado, Laura, Orellana, M. Angeles, Sanz, Francisca, Otero, Joaquin R., Oliver, Antonio, Chaves, Fernando
Tipo de recurso: artículo
Fecha de publicación:2012
País:España
Institución:Conselleria de Salut i Consum del Govern de les Illes Balears
Repositorio:Docusalut
Idioma:inglés
OAI Identifier:oai:docusalut.com:20.500.13003/13065
Acceso en línea:https://hdl.handle.net/20.500.13003/13065
Access Level:acceso abierto
Palabra clave:Cross Infection
Pseudomonas Infections
Molecular Epidemiology
Sequence Analysis, DNA
Prevalence
Pseudomonas aeruginosa
beta-Lactamases
Female
Multilocus Sequence Typing
Male
Middle Aged
Humans
DNA, Bacterial
Genotype
Aged
Bacterial Typing Techniques
Drug Resistance, Multiple, Bacterial
Spain
Anti-Bacterial Agents
Microbial Sensitivity Tests
beta-Lactamasas
Tipificación de Secuencias Multilocus
Pruebas de Sensibilidad Microbiana
Humanos
Persona de Mediana Edad
Masculino
ADN Bacteriano
Anciano
Infección Hospitalaria
Genotipo
Femenino
Prevalencia
Farmacorresistencia Bacteriana Múltiple
Análisis de Secuencia de ADN
Infecciones por Pseudomonas
Antibacterianos
Técnicas de Tipificación Bacteriana
Epidemiología Molecular
España
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spelling VIM-2-producing Multidrug-Resistant Pseudomonas aeruginosa ST175 Clone, SpainViedma, EstherJuan, CarlosVilla, JenniferBarrado, LauraOrellana, M. AngelesSanz, FranciscaOtero, Joaquin R.Oliver, AntonioChaves, FernandoCross InfectionPseudomonas InfectionsMolecular EpidemiologySequence Analysis, DNAPrevalencePseudomonas aeruginosabeta-LactamasesFemaleMultilocus Sequence TypingMaleMiddle AgedHumansDNA, BacterialGenotypeAgedBacterial Typing TechniquesDrug Resistance, Multiple, BacterialSpainAnti-Bacterial AgentsMicrobial Sensitivity Testsbeta-LactamasasTipificación de Secuencias MultilocusPruebas de Sensibilidad MicrobianaHumanosPersona de Mediana EdadMasculinoADN BacterianoAncianoInfección HospitalariaGenotipoFemeninoPrevalenciaFarmacorresistencia Bacteriana MúltipleAnálisis de Secuencia de ADNInfecciones por PseudomonasAntibacterianosTécnicas de Tipificación BacterianaPseudomonas aeruginosaEpidemiología MolecularEspañaA total of 183 patients were colonized or infected with multidrug-resistant Pseudomonas aeruginosa isolates at a hospital in Spain during 2007-2010; prevalence increased over this period from 2.8% to 15.3%. To characterize these isolates, we performed molecular epidemiologic and drug resistance analysis. Genotyping showed that 104 (56.8%) isolates belonged to a single major clone (clone B), which was identified by multilocus sequence typing as sequence type (ST) 175. This clone was initially isolated from 5 patients in 2008, and then isolated from 23 patients in 2009 and 76 patients in 2010. PCR analysis of clone B isolates identified the b/a(VIM-2) gene in all but 1 isolate, which harbored b/a(IMP-22). ST175 isolates were susceptible to only amikacin (75%) and colistin (100%). Emergence of the ST175 clone represents a major health problem because it compromises therapy for treatment of P aeruginosa nosocomial infections.Centers Disease Control & Prevention20122012-08-0120122012-08-01research articlehttp://purl.org/coar/resource_type/c_2df8fbb1info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.13003/13065reponame:Docusalutinstname:Conselleria de Salut i Consum del Govern de les Illes BalearsInglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:docusalut.com:20.500.13003/130652026-06-22T12:44:07Z
dc.title.none.fl_str_mv VIM-2-producing Multidrug-Resistant Pseudomonas aeruginosa ST175 Clone, Spain
title VIM-2-producing Multidrug-Resistant Pseudomonas aeruginosa ST175 Clone, Spain
spellingShingle VIM-2-producing Multidrug-Resistant Pseudomonas aeruginosa ST175 Clone, Spain
Viedma, Esther
Cross Infection
Pseudomonas Infections
Molecular Epidemiology
Sequence Analysis, DNA
Prevalence
Pseudomonas aeruginosa
beta-Lactamases
Female
Multilocus Sequence Typing
Male
Middle Aged
Humans
DNA, Bacterial
Genotype
Aged
Bacterial Typing Techniques
Drug Resistance, Multiple, Bacterial
Spain
Anti-Bacterial Agents
Microbial Sensitivity Tests
beta-Lactamasas
Tipificación de Secuencias Multilocus
Pruebas de Sensibilidad Microbiana
Humanos
Persona de Mediana Edad
Masculino
ADN Bacteriano
Anciano
Infección Hospitalaria
Genotipo
Femenino
Prevalencia
Farmacorresistencia Bacteriana Múltiple
Análisis de Secuencia de ADN
Infecciones por Pseudomonas
Antibacterianos
Técnicas de Tipificación Bacteriana
Pseudomonas aeruginosa
Epidemiología Molecular
España
title_short VIM-2-producing Multidrug-Resistant Pseudomonas aeruginosa ST175 Clone, Spain
title_full VIM-2-producing Multidrug-Resistant Pseudomonas aeruginosa ST175 Clone, Spain
title_fullStr VIM-2-producing Multidrug-Resistant Pseudomonas aeruginosa ST175 Clone, Spain
title_full_unstemmed VIM-2-producing Multidrug-Resistant Pseudomonas aeruginosa ST175 Clone, Spain
title_sort VIM-2-producing Multidrug-Resistant Pseudomonas aeruginosa ST175 Clone, Spain
dc.creator.none.fl_str_mv Viedma, Esther
Juan, Carlos
Villa, Jennifer
Barrado, Laura
Orellana, M. Angeles
Sanz, Francisca
Otero, Joaquin R.
Oliver, Antonio
Chaves, Fernando
author Viedma, Esther
author_facet Viedma, Esther
Juan, Carlos
Villa, Jennifer
Barrado, Laura
Orellana, M. Angeles
Sanz, Francisca
Otero, Joaquin R.
Oliver, Antonio
Chaves, Fernando
author_role author
author2 Juan, Carlos
Villa, Jennifer
Barrado, Laura
Orellana, M. Angeles
Sanz, Francisca
Otero, Joaquin R.
Oliver, Antonio
Chaves, Fernando
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv
dc.subject.none.fl_str_mv Cross Infection
Pseudomonas Infections
Molecular Epidemiology
Sequence Analysis, DNA
Prevalence
Pseudomonas aeruginosa
beta-Lactamases
Female
Multilocus Sequence Typing
Male
Middle Aged
Humans
DNA, Bacterial
Genotype
Aged
Bacterial Typing Techniques
Drug Resistance, Multiple, Bacterial
Spain
Anti-Bacterial Agents
Microbial Sensitivity Tests
beta-Lactamasas
Tipificación de Secuencias Multilocus
Pruebas de Sensibilidad Microbiana
Humanos
Persona de Mediana Edad
Masculino
ADN Bacteriano
Anciano
Infección Hospitalaria
Genotipo
Femenino
Prevalencia
Farmacorresistencia Bacteriana Múltiple
Análisis de Secuencia de ADN
Infecciones por Pseudomonas
Antibacterianos
Técnicas de Tipificación Bacteriana
Pseudomonas aeruginosa
Epidemiología Molecular
España
topic Cross Infection
Pseudomonas Infections
Molecular Epidemiology
Sequence Analysis, DNA
Prevalence
Pseudomonas aeruginosa
beta-Lactamases
Female
Multilocus Sequence Typing
Male
Middle Aged
Humans
DNA, Bacterial
Genotype
Aged
Bacterial Typing Techniques
Drug Resistance, Multiple, Bacterial
Spain
Anti-Bacterial Agents
Microbial Sensitivity Tests
beta-Lactamasas
Tipificación de Secuencias Multilocus
Pruebas de Sensibilidad Microbiana
Humanos
Persona de Mediana Edad
Masculino
ADN Bacteriano
Anciano
Infección Hospitalaria
Genotipo
Femenino
Prevalencia
Farmacorresistencia Bacteriana Múltiple
Análisis de Secuencia de ADN
Infecciones por Pseudomonas
Antibacterianos
Técnicas de Tipificación Bacteriana
Pseudomonas aeruginosa
Epidemiología Molecular
España
description A total of 183 patients were colonized or infected with multidrug-resistant Pseudomonas aeruginosa isolates at a hospital in Spain during 2007-2010; prevalence increased over this period from 2.8% to 15.3%. To characterize these isolates, we performed molecular epidemiologic and drug resistance analysis. Genotyping showed that 104 (56.8%) isolates belonged to a single major clone (clone B), which was identified by multilocus sequence typing as sequence type (ST) 175. This clone was initially isolated from 5 patients in 2008, and then isolated from 23 patients in 2009 and 76 patients in 2010. PCR analysis of clone B isolates identified the b/a(VIM-2) gene in all but 1 isolate, which harbored b/a(IMP-22). ST175 isolates were susceptible to only amikacin (75%) and colistin (100%). Emergence of the ST175 clone represents a major health problem because it compromises therapy for treatment of P aeruginosa nosocomial infections.
publishDate 2012
dc.date.none.fl_str_mv 2012
2012-08-01
2012
2012-08-01
dc.type.none.fl_str_mv research article
http://purl.org/coar/resource_type/c_2df8fbb1
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.13003/13065
url https://hdl.handle.net/20.500.13003/13065
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Centers Disease Control & Prevention
publisher.none.fl_str_mv Centers Disease Control & Prevention
dc.source.none.fl_str_mv reponame:Docusalut
instname:Conselleria de Salut i Consum del Govern de les Illes Balears
instname_str Conselleria de Salut i Consum del Govern de les Illes Balears
reponame_str Docusalut
collection Docusalut
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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