A gene regulatory network critical for axillary bud dormancy directly controlled by Arabidopsis BRANCHED1

The Arabidopsis thaliana transcription factor BRANCHED1 (BRC1) plays a pivotal role in the control of shoot branching as it integrates environmental and endogenous signals that influence axillary bud growth. Despite its remarkable activity as a growth inhibitor, the mechanisms by which BRC1 promotes...

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Autores: van Es, Sam W., Muñoz-Gasca, Aitor, Romero Campero, Francisco José, González-Grandío, Eduardo, de Los Reyes, Pedro, Tarancón, Carlos, van Dijk, Aalt D. J., van Esse, Wilma, Pascual-Garcia, Alberto, Angenent, Gerco C., Immink, Richard G. H., Cubas, Pilar
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:España
Institución:Universidad de Sevilla (US)
Repositorio:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/174709
Acceso en línea:https://hdl.handle.net/11441/174709
https://doi.org/10.1111/nph.19420
Access Level:acceso abierto
Palabra clave:Arabidopsis
Axillary bud dormancy
BRANCHED1
Gene regulatory network
Shoot branching
Systems biology
TCP
Transcription factors
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spelling A gene regulatory network critical for axillary bud dormancy directly controlled by Arabidopsis BRANCHED1van Es, Sam W.Muñoz-Gasca, AitorRomero Campero, Francisco JoséGonzález-Grandío, Eduardode Los Reyes, PedroTarancón, Carlosvan Dijk, Aalt D. J.van Esse, WilmaPascual-Garcia, AlbertoAngenent, Gerco C.Immink, Richard G. H.Cubas, PilarArabidopsisAxillary bud dormancyBRANCHED1Gene regulatory networkShoot branchingSystems biologyTCPTranscription factorsThe Arabidopsis thaliana transcription factor BRANCHED1 (BRC1) plays a pivotal role in the control of shoot branching as it integrates environmental and endogenous signals that influence axillary bud growth. Despite its remarkable activity as a growth inhibitor, the mechanisms by which BRC1 promotes bud dormancy are largely unknown. We determined the genome-wide BRC1 binding sites in vivo and combined these with transcriptomic data and gene co-expression analyses to identify bona fide BRC1 direct targets. Next, we integrated multi-omics data to infer the BRC1 gene regulatory network (GRN) and used graph theory techniques to find network motifs that control the GRN dynamics. We generated an open online tool to interrogate this network. A group of BRC1 target genes encoding transcription factors (BTFs) orchestrate this intricate transcriptional network enriched in abscisic acid-related components. Promoter::b-GLUCURONIDASE transgenic lines confirmed that BTFs are expressed in axillary buds. Transient co-expression assays and studies in planta using mutant lines validated the role of BTFs in modulating the GRN and promoting bud dormancy. This knowledge provides access to the developmental mechanisms that regulate shootbranching and helps identify candidate genes to use as tools to adapt plant architecture and crop production to ever-changing environmental conditions.WileyCiencias de la Computación e Inteligencia Artificial2024info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/11441/174709https://doi.org/10.1111/nph.19420reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésNew Phytologist, 241 (3), 1193-1209.https://nph.onlinelibrary.wiley.com/doi/10.1111/nph.19420info:eu-repo/semantics/openAccessoai:idus.us.es:11441/1747092026-06-17T12:51:07Z
dc.title.none.fl_str_mv A gene regulatory network critical for axillary bud dormancy directly controlled by Arabidopsis BRANCHED1
title A gene regulatory network critical for axillary bud dormancy directly controlled by Arabidopsis BRANCHED1
spellingShingle A gene regulatory network critical for axillary bud dormancy directly controlled by Arabidopsis BRANCHED1
van Es, Sam W.
Arabidopsis
Axillary bud dormancy
BRANCHED1
Gene regulatory network
Shoot branching
Systems biology
TCP
Transcription factors
title_short A gene regulatory network critical for axillary bud dormancy directly controlled by Arabidopsis BRANCHED1
title_full A gene regulatory network critical for axillary bud dormancy directly controlled by Arabidopsis BRANCHED1
title_fullStr A gene regulatory network critical for axillary bud dormancy directly controlled by Arabidopsis BRANCHED1
title_full_unstemmed A gene regulatory network critical for axillary bud dormancy directly controlled by Arabidopsis BRANCHED1
title_sort A gene regulatory network critical for axillary bud dormancy directly controlled by Arabidopsis BRANCHED1
dc.creator.none.fl_str_mv van Es, Sam W.
Muñoz-Gasca, Aitor
Romero Campero, Francisco José
González-Grandío, Eduardo
de Los Reyes, Pedro
Tarancón, Carlos
van Dijk, Aalt D. J.
van Esse, Wilma
Pascual-Garcia, Alberto
Angenent, Gerco C.
Immink, Richard G. H.
Cubas, Pilar
author van Es, Sam W.
author_facet van Es, Sam W.
Muñoz-Gasca, Aitor
Romero Campero, Francisco José
González-Grandío, Eduardo
de Los Reyes, Pedro
Tarancón, Carlos
van Dijk, Aalt D. J.
van Esse, Wilma
Pascual-Garcia, Alberto
Angenent, Gerco C.
Immink, Richard G. H.
Cubas, Pilar
author_role author
author2 Muñoz-Gasca, Aitor
Romero Campero, Francisco José
González-Grandío, Eduardo
de Los Reyes, Pedro
Tarancón, Carlos
van Dijk, Aalt D. J.
van Esse, Wilma
Pascual-Garcia, Alberto
Angenent, Gerco C.
Immink, Richard G. H.
Cubas, Pilar
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ciencias de la Computación e Inteligencia Artificial
dc.subject.none.fl_str_mv Arabidopsis
Axillary bud dormancy
BRANCHED1
Gene regulatory network
Shoot branching
Systems biology
TCP
Transcription factors
topic Arabidopsis
Axillary bud dormancy
BRANCHED1
Gene regulatory network
Shoot branching
Systems biology
TCP
Transcription factors
description The Arabidopsis thaliana transcription factor BRANCHED1 (BRC1) plays a pivotal role in the control of shoot branching as it integrates environmental and endogenous signals that influence axillary bud growth. Despite its remarkable activity as a growth inhibitor, the mechanisms by which BRC1 promotes bud dormancy are largely unknown. We determined the genome-wide BRC1 binding sites in vivo and combined these with transcriptomic data and gene co-expression analyses to identify bona fide BRC1 direct targets. Next, we integrated multi-omics data to infer the BRC1 gene regulatory network (GRN) and used graph theory techniques to find network motifs that control the GRN dynamics. We generated an open online tool to interrogate this network. A group of BRC1 target genes encoding transcription factors (BTFs) orchestrate this intricate transcriptional network enriched in abscisic acid-related components. Promoter::b-GLUCURONIDASE transgenic lines confirmed that BTFs are expressed in axillary buds. Transient co-expression assays and studies in planta using mutant lines validated the role of BTFs in modulating the GRN and promoting bud dormancy. This knowledge provides access to the developmental mechanisms that regulate shootbranching and helps identify candidate genes to use as tools to adapt plant architecture and crop production to ever-changing environmental conditions.
publishDate 2024
dc.date.none.fl_str_mv 2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11441/174709
https://doi.org/10.1111/nph.19420
url https://hdl.handle.net/11441/174709
https://doi.org/10.1111/nph.19420
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv New Phytologist, 241 (3), 1193-1209.
https://nph.onlinelibrary.wiley.com/doi/10.1111/nph.19420
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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