Allergen expression in the European house dust mite Dermatophagoides pteronyssinus throughout development and response to environmental conditions

10 p.-2 fig.-5 tab.

Bibliographic Details
Authors: Vidal Quist, José Cristian, Ortego, Félix, Lombardero, Manuel, Castañera, Pedro, Hernández-Crespo, Pedro
Format: article
Status:Published version
Publication Date:2015
Country:España
Institution:Consejo Superior de Investigaciones Científicas (CSIC)
Repository:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/361923
Online Access:http://hdl.handle.net/10261/361923
Access Level:Open access
Keyword:Allergen profile
ELISA
HDM
Allergenic extracts
Allergy
Mite culture
Real-time quantitative PCR
Tropomyosin
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oai_identifier_str oai:digital.csic.es:10261/361923
network_acronym_str ES
network_name_str España
repository_id_str
spelling Allergen expression in the European house dust mite Dermatophagoides pteronyssinus throughout development and response to environmental conditionsVidal Quist, José CristianOrtego, FélixLombardero, ManuelCastañera, PedroHernández-Crespo, PedroAllergen profileELISAHDMAllergenic extractsAllergyMite cultureReal-time quantitative PCRTropomyosin10 p.-2 fig.-5 tab.House dust mites are a major source of allergy worldwide. While diagnosis and treatment based on mite extracts have remarkably advanced, little information exists on the expression of allergens in mites. We have studied gene expression of eight Dermatophagoides pteronyssinus (Trouessart) (Acari: Pyroglyphidae) allergens (Der p 1, 2, 3, 4, 5, 7, 10 and 21). All allergens showed higher transcription in nymphs compared with larvae or adults, with the only exception of Der p 10. The transcription of Der p 4 and Der p 10, together with the transcription and protein ratios Der p 1 to Der p 2, were higher in males than in females. One-week exposure of mite cultures to 16 or 35 °C (versus 24 °C) or low RH (44% versus 76%) significantly influenced the allergen gene transcription profile. Our results demonstrate that allergen expression is quantitatively and/or qualitatively influenced by mite development and sex, as well as by the environment. We suggest that monitoring allergen gene expression may be a useful tool to assist the optimization of mite cultures in the production of standardized allergenic extracts for clinical use.Peer reviewedJohn Wiley & SonsVidal Quist, José Cristian [0000-0002-3931-2592]Ortego, Félix [0000-0002-7778-8508]Lombardero, Manuel [0000-0001-5386-5321]Hernández-Crespo, Pedro [0000-0001-9350-3640]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202420242015info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/361923reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://doi.org/10.1111/mve.12102Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3619232026-05-22T06:33:51Z
dc.title.none.fl_str_mv Allergen expression in the European house dust mite Dermatophagoides pteronyssinus throughout development and response to environmental conditions
title Allergen expression in the European house dust mite Dermatophagoides pteronyssinus throughout development and response to environmental conditions
spellingShingle Allergen expression in the European house dust mite Dermatophagoides pteronyssinus throughout development and response to environmental conditions
Vidal Quist, José Cristian
Allergen profile
ELISA
HDM
Allergenic extracts
Allergy
Mite culture
Real-time quantitative PCR
Tropomyosin
title_short Allergen expression in the European house dust mite Dermatophagoides pteronyssinus throughout development and response to environmental conditions
title_full Allergen expression in the European house dust mite Dermatophagoides pteronyssinus throughout development and response to environmental conditions
title_fullStr Allergen expression in the European house dust mite Dermatophagoides pteronyssinus throughout development and response to environmental conditions
title_full_unstemmed Allergen expression in the European house dust mite Dermatophagoides pteronyssinus throughout development and response to environmental conditions
title_sort Allergen expression in the European house dust mite Dermatophagoides pteronyssinus throughout development and response to environmental conditions
dc.creator.none.fl_str_mv Vidal Quist, José Cristian
Ortego, Félix
Lombardero, Manuel
Castañera, Pedro
Hernández-Crespo, Pedro
author Vidal Quist, José Cristian
author_facet Vidal Quist, José Cristian
Ortego, Félix
Lombardero, Manuel
Castañera, Pedro
Hernández-Crespo, Pedro
author_role author
author2 Ortego, Félix
Lombardero, Manuel
Castañera, Pedro
Hernández-Crespo, Pedro
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Vidal Quist, José Cristian [0000-0002-3931-2592]
Ortego, Félix [0000-0002-7778-8508]
Lombardero, Manuel [0000-0001-5386-5321]
Hernández-Crespo, Pedro [0000-0001-9350-3640]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Allergen profile
ELISA
HDM
Allergenic extracts
Allergy
Mite culture
Real-time quantitative PCR
Tropomyosin
topic Allergen profile
ELISA
HDM
Allergenic extracts
Allergy
Mite culture
Real-time quantitative PCR
Tropomyosin
description 10 p.-2 fig.-5 tab.
publishDate 2015
dc.date.none.fl_str_mv 2015
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/361923
url http://hdl.handle.net/10261/361923
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.1111/mve.12102
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv John Wiley & Sons
publisher.none.fl_str_mv John Wiley & Sons
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869405425016766464
score 15,811543

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