Valorization of onion waste by obtaining extracts rich in phenolic compounds and feasibility of its therapeutic use on colon cancer

In this study, the total phenolic content, the antioxidant and antiproliferative activities of onion waste extracts were characterized. Some phenolic compounds present in the extracts were also identified and quantified by HPLC-DAD. Additionally, an in-silico analysis was performed to identify the p...

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Autores: Paesa, Mónica, Pires Nogueira, Danielle, Velderrain-Rodríguez, Gustavo, Esparza Catalán, Irene, Jiménez Moreno, Nerea, Mendoza, Gracia, Osada, Jesús, Martín-Belloso, Olga, Rodríguez Yoldi, María Jesús, Ancín Azpilicueta, Carmen
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Institución:Universidad Pública de Navarra
Repositorio:Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
OAI Identifier:oai:academica-e.unavarra.es:2454/43208
Acceso en línea:https://hdl.handle.net/2454/43208
Access Level:acceso abierto
Palabra clave:Allium cepa L. by-products
Caco-2 cells
Circular economy
Intestine
Protocatechuic acid
ROS
Vegetable extracts
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spelling Valorization of onion waste by obtaining extracts rich in phenolic compounds and feasibility of its therapeutic use on colon cancerPaesa, MónicaPires Nogueira, DanielleVelderrain-Rodríguez, GustavoEsparza Catalán, IreneJiménez Moreno, NereaMendoza, GraciaOsada, JesúsMartín-Belloso, OlgaRodríguez Yoldi, María JesúsAncín Azpilicueta, CarmenAllium cepa L. by-productsCaco-2 cellsCircular economyIntestineProtocatechuic acidROSVegetable extractsIn this study, the total phenolic content, the antioxidant and antiproliferative activities of onion waste extracts were characterized. Some phenolic compounds present in the extracts were also identified and quantified by HPLC-DAD. Additionally, an in-silico analysis was performed to identify the phenolic compounds with the highest intestinal absorption and Caco-2 permeability. The onion extract possessed a high amount of phenolic compounds (177 ± 9 mg/g extract) and had an effective antioxidant capacity measured by ABTS, FRAP and DPPH assays. Regarding the antiproliferative activity, the onion extracts produced cell cycle arrest in the S phase with p53 activation, intrinsic apoptosis (mitochondrial membrane potential modification) and caspase 3 activation. Likewise, onion waste increased intracellular ROS with possible NF-kB activation causing a proteasome down regulation. In addition, the extracts protected the intestine against oxidative stress induced by H2O2. According to the in-silico analysis, these results could be related to the higher Caco-2 permeability to protocatechuic acid. Therefore, this study provides new insights regarding the potential use of these types of extract as functional ingredients with antioxidant and antiproliferative properties and as medicinal agents in diseases related to oxidative stress, such as cancer. In addition, its valorization would contribute to the circular economy.This work was supported by the Grants: CIBEROBN, CB06/03/1012, 2008, Ministerio de Ciencia e Innovación-Fondo Europeo de Desarrollo Regional (PID2019-104915RB-I00), Fondo Social Europeo-Gobierno de Aragón (B16_20R) and SUDOE (Redvalue, SOE1/PI/E0123). This research was also funded by EUROPEAN UNIONS H2020 research and innovation program under Marie Sklodowska-Curie grant agreement number 801586. G.M. gratefully acknowledges the support from the Miguel Servet Program (MS19/00092; Instituto de Salud Carlos III). M.P. acknowledges the support from the Gobierno de Aragón fellowship (CUS/581/2020).MDPIInstitute for Advanced Materials and Mathematics - INAMAT22022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2454/43208reponame:Academica-e. Repositorio Institucional de la Universidad Pública de Navarrainstname:Universidad Pública de NavarraInglésinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-104915RB-I00info:eu-repo/grantAgreement/European Commission/Horizon 2020 Framework Programme/801586info:eu-repo/semantics/openAccessoai:academica-e.unavarra.es:2454/432082026-06-17T12:41:47Z
dc.title.none.fl_str_mv Valorization of onion waste by obtaining extracts rich in phenolic compounds and feasibility of its therapeutic use on colon cancer
title Valorization of onion waste by obtaining extracts rich in phenolic compounds and feasibility of its therapeutic use on colon cancer
spellingShingle Valorization of onion waste by obtaining extracts rich in phenolic compounds and feasibility of its therapeutic use on colon cancer
Paesa, Mónica
Allium cepa L. by-products
Caco-2 cells
Circular economy
Intestine
Protocatechuic acid
ROS
Vegetable extracts
title_short Valorization of onion waste by obtaining extracts rich in phenolic compounds and feasibility of its therapeutic use on colon cancer
title_full Valorization of onion waste by obtaining extracts rich in phenolic compounds and feasibility of its therapeutic use on colon cancer
title_fullStr Valorization of onion waste by obtaining extracts rich in phenolic compounds and feasibility of its therapeutic use on colon cancer
title_full_unstemmed Valorization of onion waste by obtaining extracts rich in phenolic compounds and feasibility of its therapeutic use on colon cancer
title_sort Valorization of onion waste by obtaining extracts rich in phenolic compounds and feasibility of its therapeutic use on colon cancer
dc.creator.none.fl_str_mv Paesa, Mónica
Pires Nogueira, Danielle
Velderrain-Rodríguez, Gustavo
Esparza Catalán, Irene
Jiménez Moreno, Nerea
Mendoza, Gracia
Osada, Jesús
Martín-Belloso, Olga
Rodríguez Yoldi, María Jesús
Ancín Azpilicueta, Carmen
author Paesa, Mónica
author_facet Paesa, Mónica
Pires Nogueira, Danielle
Velderrain-Rodríguez, Gustavo
Esparza Catalán, Irene
Jiménez Moreno, Nerea
Mendoza, Gracia
Osada, Jesús
Martín-Belloso, Olga
Rodríguez Yoldi, María Jesús
Ancín Azpilicueta, Carmen
author_role author
author2 Pires Nogueira, Danielle
Velderrain-Rodríguez, Gustavo
Esparza Catalán, Irene
Jiménez Moreno, Nerea
Mendoza, Gracia
Osada, Jesús
Martín-Belloso, Olga
Rodríguez Yoldi, María Jesús
Ancín Azpilicueta, Carmen
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Institute for Advanced Materials and Mathematics - INAMAT2
dc.subject.none.fl_str_mv Allium cepa L. by-products
Caco-2 cells
Circular economy
Intestine
Protocatechuic acid
ROS
Vegetable extracts
topic Allium cepa L. by-products
Caco-2 cells
Circular economy
Intestine
Protocatechuic acid
ROS
Vegetable extracts
description In this study, the total phenolic content, the antioxidant and antiproliferative activities of onion waste extracts were characterized. Some phenolic compounds present in the extracts were also identified and quantified by HPLC-DAD. Additionally, an in-silico analysis was performed to identify the phenolic compounds with the highest intestinal absorption and Caco-2 permeability. The onion extract possessed a high amount of phenolic compounds (177 ± 9 mg/g extract) and had an effective antioxidant capacity measured by ABTS, FRAP and DPPH assays. Regarding the antiproliferative activity, the onion extracts produced cell cycle arrest in the S phase with p53 activation, intrinsic apoptosis (mitochondrial membrane potential modification) and caspase 3 activation. Likewise, onion waste increased intracellular ROS with possible NF-kB activation causing a proteasome down regulation. In addition, the extracts protected the intestine against oxidative stress induced by H2O2. According to the in-silico analysis, these results could be related to the higher Caco-2 permeability to protocatechuic acid. Therefore, this study provides new insights regarding the potential use of these types of extract as functional ingredients with antioxidant and antiproliferative properties and as medicinal agents in diseases related to oxidative stress, such as cancer. In addition, its valorization would contribute to the circular economy.
publishDate 2022
dc.date.none.fl_str_mv 2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2454/43208
url https://hdl.handle.net/2454/43208
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-104915RB-I00
info:eu-repo/grantAgreement/European Commission/Horizon 2020 Framework Programme/801586
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
instname:Universidad Pública de Navarra
instname_str Universidad Pública de Navarra
reponame_str Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
collection Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
repository.name.fl_str_mv
repository.mail.fl_str_mv
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