Fluorescence spectroscopy: a rapid tool for assessing tetracycline resistance in Bifidobacterium longum

[EN]The tetracycline uptake kinetics of 35 Bifidobacterium longum strains isolated from the human gastrointestinal tract were examined by fluorescence spectroscopy, and the suitability of the technique as a screening tool of tetracycline resistance or susceptibility was determined. The strains were...

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Autores: Ammor, Mohammed Salim, Flórez, Ana Belén, Margolles Barros, Abelardo, Mayo Pérez, Baltasar
Tipo de recurso: artículo
Fecha de publicación:2006
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/6986
Acceso en línea:http://hdl.handle.net/10261/6986
Access Level:acceso abierto
Palabra clave:Tetraciclina
Bifidobacterias
Antibióticos
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spelling Fluorescence spectroscopy: a rapid tool for assessing tetracycline resistance in Bifidobacterium longumAmmor, Mohammed SalimFlórez, Ana BelénMargolles Barros, AbelardoMayo Pérez, BaltasarTetraciclinaBifidobacteriasAntibióticos[EN]The tetracycline uptake kinetics of 35 Bifidobacterium longum strains isolated from the human gastrointestinal tract were examined by fluorescence spectroscopy, and the suitability of the technique as a screening tool of tetracycline resistance or susceptibility was determined. The strains were first grouped into three classes based on their corresponding minimum inhibitory concentrations (MICs) of tetracycline, as established by the microdilution method: susceptible (MICs ≤1 µg mL-1), semi-resistant (MICs between 1 and ≤32 µg mL-1), and resistant strains (MICs ≥32 µg mL-1). The kinetics of tetracycline uptake for the strains in each resistance group were then analyzed over a 20 min period by fluorescence spectroscopy (absorbance wavelength 524 nm, excitation wavelength 400 nm) in a buffer system containing 100 µg mL-1 tetracycline. Principal component analysis and factorial discriminant analysis of the results showed excellent distinction among susceptible, semi-resistant, and resistant strains. The proposed method provides a powerful and convenient means of rapidly screening tetracycline resistance in B. longum.[FR]Les cinétiques d'absorption de la tétracycline chez 35 souches de Bifidobacterium longum isolées du tractus gastro-intestinal humain furent examinées par spectroscopie à fluorescence et la performance de cette technique comme outil de criblage de la résistance ou susceptibilité à la tétracycline fut déterminée. Les souches furent d'abord groupées en trois classes selon leurs concentrations inhibitrices minimales (CMI) à la tétracycline correspondantes telles que déterminées par la méthode de microdilution: souches susceptibles (CMI ≤1 µg mL-1), semi-resistante (CMI entre 1 et ≤32 µg mL-1) et résistantes (CMI ≥32 µg mL-1). Les cinétiques d'absorption de la tétracycline pour les souches dans chacun des groupes de résistance furent par la suite analysée sur une durée de 20 minutes par spectroscopie à fluorescence (longueur d'onde d'absorbance de 524 nm, longueur d'onde d'excitation de 400 nm) dans un système tamponné contenant 100 µg mL-1 de tétracycline. Une analyse des composantes principales et une analyse factorielle du discriminant des résultats ont démontré une excellente distinction entre les souches susceptibles, semi-résistantes et résistantes. La méthode proposée fournit un moyen performant et pratique pour cribler rapidement la résistance à la tétracycline chez B. longum.This work was supported by a projet with in the sixth Framework Program of the EU (ACE-ART, ref 506214). M.S. Ammon was the recipient of a postdoctoral fellowship from the Secretaría de Estado de Universidades e Investigación, Spanish Ministry of Education and Science (ref. SB2004-0165)Peer reviewedNational Research Council CanadaEuropean CommissionMinisterio de Educación y Ciencia (España)200820082006info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501166339 bytesapplication/pdfhttp://hdl.handle.net/10261/6986reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglésinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/69862026-05-22T06:33:51Z
dc.title.none.fl_str_mv Fluorescence spectroscopy: a rapid tool for assessing tetracycline resistance in Bifidobacterium longum
title Fluorescence spectroscopy: a rapid tool for assessing tetracycline resistance in Bifidobacterium longum
spellingShingle Fluorescence spectroscopy: a rapid tool for assessing tetracycline resistance in Bifidobacterium longum
Ammor, Mohammed Salim
Tetraciclina
Bifidobacterias
Antibióticos
title_short Fluorescence spectroscopy: a rapid tool for assessing tetracycline resistance in Bifidobacterium longum
title_full Fluorescence spectroscopy: a rapid tool for assessing tetracycline resistance in Bifidobacterium longum
title_fullStr Fluorescence spectroscopy: a rapid tool for assessing tetracycline resistance in Bifidobacterium longum
title_full_unstemmed Fluorescence spectroscopy: a rapid tool for assessing tetracycline resistance in Bifidobacterium longum
title_sort Fluorescence spectroscopy: a rapid tool for assessing tetracycline resistance in Bifidobacterium longum
dc.creator.none.fl_str_mv Ammor, Mohammed Salim
Flórez, Ana Belén
Margolles Barros, Abelardo
Mayo Pérez, Baltasar
author Ammor, Mohammed Salim
author_facet Ammor, Mohammed Salim
Flórez, Ana Belén
Margolles Barros, Abelardo
Mayo Pérez, Baltasar
author_role author
author2 Flórez, Ana Belén
Margolles Barros, Abelardo
Mayo Pérez, Baltasar
author2_role author
author
author
dc.contributor.none.fl_str_mv European Commission
Ministerio de Educación y Ciencia (España)
dc.subject.none.fl_str_mv Tetraciclina
Bifidobacterias
Antibióticos
topic Tetraciclina
Bifidobacterias
Antibióticos
description [EN]The tetracycline uptake kinetics of 35 Bifidobacterium longum strains isolated from the human gastrointestinal tract were examined by fluorescence spectroscopy, and the suitability of the technique as a screening tool of tetracycline resistance or susceptibility was determined. The strains were first grouped into three classes based on their corresponding minimum inhibitory concentrations (MICs) of tetracycline, as established by the microdilution method: susceptible (MICs ≤1 µg mL-1), semi-resistant (MICs between 1 and ≤32 µg mL-1), and resistant strains (MICs ≥32 µg mL-1). The kinetics of tetracycline uptake for the strains in each resistance group were then analyzed over a 20 min period by fluorescence spectroscopy (absorbance wavelength 524 nm, excitation wavelength 400 nm) in a buffer system containing 100 µg mL-1 tetracycline. Principal component analysis and factorial discriminant analysis of the results showed excellent distinction among susceptible, semi-resistant, and resistant strains. The proposed method provides a powerful and convenient means of rapidly screening tetracycline resistance in B. longum.
publishDate 2006
dc.date.none.fl_str_mv 2006
2008
2008
dc.type.none.fl_str_mv info:eu-repo/semantics/article
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format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/6986
url http://hdl.handle.net/10261/6986
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 166339 bytes
application/pdf
dc.publisher.none.fl_str_mv National Research Council Canada
publisher.none.fl_str_mv National Research Council Canada
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
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