Polyphenols bioaccessibility and bioavailability assessment in ipecac infusion using a combined assay of simulated in vitro digestion and Caco-2 cell model

In this report, we investigated for the first time the total polyphenols content (TPC) and antioxidant activity before and after digestion of Carapichea ipecacuanha root infusion, better known as ipecac, prepared at different concentrations. An in vitro digestion system coupled to a Caco-2 cell mode...

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Detalles Bibliográficos
Autores: Ben Hlel, T., Borges, Thays H., Rueda, Ascensión, Smaali, I., Marzouki, M. N., Seiquer, Isabel
Tipo de recurso: artículo
Fecha de publicación:2019
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/184900
Acceso en línea:http://hdl.handle.net/10261/184900
Access Level:acceso abierto
Palabra clave:Caco-2 cells
in vitro digestión
ipecac infusión
polyphenols.
Antioxidant activity
Bioavailability
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spelling Polyphenols bioaccessibility and bioavailability assessment in ipecac infusion using a combined assay of simulated in vitro digestion and Caco-2 cell modelBen Hlel, T.Borges, Thays H.Rueda, AscensiónSmaali, I.Marzouki, M. N.Seiquer, IsabelCaco-2 cellsin vitro digestiónipecac infusiónpolyphenols.Antioxidant activityBioavailabilityIn this report, we investigated for the first time the total polyphenols content (TPC) and antioxidant activity before and after digestion of Carapichea ipecacuanha root infusion, better known as ipecac, prepared at different concentrations. An in vitro digestion system coupled to a Caco-2 cell model was applied to study the bioavailability of antioxidant compounds. The ability of ipecac bioaccessible fractions to inhibit reactive oxygen species (ROS) generation at cellular level was also evaluated. The findings revealed that water volume of 50 mL g-1 of sample provided the maximum yield of extraction of TPC and antioxidant activity. Polyphenols increased in content and activity after digestion and they were highly bioavailable (75% of intestinal absorption). Polyphenols were also present in the residual parts which indicate a possible local activity. Results also suggest that ipecac infusion could represent a promising source of effective bioavailable antioxidants to be exploited in functional foods field.This study was supported by the financial project of LIP‐MB laboratory LR11ES24 of INSAT, University of Carthage and by the University of Tunis El Manar, Ministry of Higher Education and Scientific Research of Tunisia through the scholarship support provided to the first author. We are grateful to Dr. Chokri Messaoud for the identification of the plant.Peer ReviewedJohn Wiley & SonsUniversité de Carthage (Tunisie)Ministère de l’Enseignement Supérieur et de la Recherche Scientifique (Tunisie)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2019201920192019info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/184900reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://ifst.onlinelibrary.wiley.com/doi/abs/10.1111/ijfs.14023Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1849002026-05-22T06:33:51Z
dc.title.none.fl_str_mv Polyphenols bioaccessibility and bioavailability assessment in ipecac infusion using a combined assay of simulated in vitro digestion and Caco-2 cell model
title Polyphenols bioaccessibility and bioavailability assessment in ipecac infusion using a combined assay of simulated in vitro digestion and Caco-2 cell model
spellingShingle Polyphenols bioaccessibility and bioavailability assessment in ipecac infusion using a combined assay of simulated in vitro digestion and Caco-2 cell model
Ben Hlel, T.
Caco-2 cells
in vitro digestión
ipecac infusión
polyphenols.
Antioxidant activity
Bioavailability
title_short Polyphenols bioaccessibility and bioavailability assessment in ipecac infusion using a combined assay of simulated in vitro digestion and Caco-2 cell model
title_full Polyphenols bioaccessibility and bioavailability assessment in ipecac infusion using a combined assay of simulated in vitro digestion and Caco-2 cell model
title_fullStr Polyphenols bioaccessibility and bioavailability assessment in ipecac infusion using a combined assay of simulated in vitro digestion and Caco-2 cell model
title_full_unstemmed Polyphenols bioaccessibility and bioavailability assessment in ipecac infusion using a combined assay of simulated in vitro digestion and Caco-2 cell model
title_sort Polyphenols bioaccessibility and bioavailability assessment in ipecac infusion using a combined assay of simulated in vitro digestion and Caco-2 cell model
dc.creator.none.fl_str_mv Ben Hlel, T.
Borges, Thays H.
Rueda, Ascensión
Smaali, I.
Marzouki, M. N.
Seiquer, Isabel
author Ben Hlel, T.
author_facet Ben Hlel, T.
Borges, Thays H.
Rueda, Ascensión
Smaali, I.
Marzouki, M. N.
Seiquer, Isabel
author_role author
author2 Borges, Thays H.
Rueda, Ascensión
Smaali, I.
Marzouki, M. N.
Seiquer, Isabel
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Université de Carthage (Tunisie)
Ministère de l’Enseignement Supérieur et de la Recherche Scientifique (Tunisie)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Caco-2 cells
in vitro digestión
ipecac infusión
polyphenols.
Antioxidant activity
Bioavailability
topic Caco-2 cells
in vitro digestión
ipecac infusión
polyphenols.
Antioxidant activity
Bioavailability
description In this report, we investigated for the first time the total polyphenols content (TPC) and antioxidant activity before and after digestion of Carapichea ipecacuanha root infusion, better known as ipecac, prepared at different concentrations. An in vitro digestion system coupled to a Caco-2 cell model was applied to study the bioavailability of antioxidant compounds. The ability of ipecac bioaccessible fractions to inhibit reactive oxygen species (ROS) generation at cellular level was also evaluated. The findings revealed that water volume of 50 mL g-1 of sample provided the maximum yield of extraction of TPC and antioxidant activity. Polyphenols increased in content and activity after digestion and they were highly bioavailable (75% of intestinal absorption). Polyphenols were also present in the residual parts which indicate a possible local activity. Results also suggest that ipecac infusion could represent a promising source of effective bioavailable antioxidants to be exploited in functional foods field.
publishDate 2019
dc.date.none.fl_str_mv 2019
2019
2019
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/184900
url http://hdl.handle.net/10261/184900
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://ifst.onlinelibrary.wiley.com/doi/abs/10.1111/ijfs.14023
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv John Wiley & Sons
publisher.none.fl_str_mv John Wiley & Sons
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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