Red LED light acts on the mitochondrial electron chain of mammalian sperm via light-time exposure-dependent mechanisms

This work analyzes the effects of red LED light on mammalian sperm mitochondrial function, using the pig as an animal model. Liquid-stored pig semen was stimulated with red-light for 1, 5 and 10 min in the presence or absence of oligomycin A, a specific inhibitor of mitochondrial ATP synthase, or ca...

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Authors: Blanco Prieto, Olga, Catalán, Jaime, Trujillo Rojas, Lina, Peña García, Àlex, Rivera del Álamo, Ma. Montserrat, Llavanera, Marc, Bonet, Sergi, Fernández Novell, Josep Maria, Yeste Oliveras, Marc, Rodríguez Gil, Joan Enric
Format: article
Status:Published version
Publication Date:2020
Country:España
Institution:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repository:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10256/18733
Online Access:http://hdl.handle.net/10256/18733
Access Level:Open access
Keyword:Espermatozoides
Mitocondris
Spermatozoa
Mitochondria
Citocrom c
Cytochrome c
Porcs -- Espermatozoides
Swine -- Spermatozoa
id ES_2cefbda90ff3e647d25ef518ecc2e7c9
oai_identifier_str oai:recercat.cat:10256/18733
network_acronym_str ES
network_name_str España
repository_id_str
spelling Red LED light acts on the mitochondrial electron chain of mammalian sperm via light-time exposure-dependent mechanismsBlanco Prieto, OlgaCatalán, JaimeTrujillo Rojas, LinaPeña García, ÀlexRivera del Álamo, Ma. MontserratLlavanera, MarcBonet, SergiFernández Novell, Josep MariaYeste Oliveras, MarcRodríguez Gil, Joan EnricEspermatozoidesMitocondrisSpermatozoaMitochondriaCitocrom cCytochrome cPorcs -- EspermatozoidesSwine -- SpermatozoaThis work analyzes the effects of red LED light on mammalian sperm mitochondrial function, using the pig as an animal model. Liquid-stored pig semen was stimulated with red-light for 1, 5 and 10 min in the presence or absence of oligomycin A, a specific inhibitor of mitochondrial ATP synthase, or carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone (FCCP), a specific disruptor of mitochondrial electron chain. Whereas exposure for 1 and 5 min significantly (p < 0.05) decreased total motility and intracellular ATP levels, irradiation for 10 min induced the opposite effect. Oligomycin A abolished the light-effects on intracellular ATP levels, O2 consumption and mitochondrial membrane potential, whereas compared to non-irradiated samples, FCCP significantly (p < 0.05) increased O2 consumption when sperm were irradiated for 1 min. Both oligomycin A and FCCP significantly (p < 0.05) decreased total motility. Red-light increased cytochrome c oxidase activity with a maximal effect after 5 min of irradiation, which was abolished by both oligomycin A and FCCP. In conclusion, red-light modulates sperm mitochondrial function via electron chain activity in an exposition, time-dependent mannerThis work has been supported by Grants RYC-2014-15581 and AGL2017-88329-R (Ministry of Science and Innovation, Spain), and 2017-SGR-1229 (Regional Government of Catalonia, Spain)Agencia Estatal de InvestigaciónMinisterio de Economía y Competitividad (Espanya)2020info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionpeer-reviewedapplication/pdfhttp://hdl.handle.net/10256/18733http://hdl.handle.net/10256/18733Cells, 2020, vol. 9, núm. 12, p. 2546Articles publicats (D-B)Blanco Prieto, Olga Catalán, Jaime Trujillo Rojas, Lina Peña García, Àlex Rivera del Álamo, Ma. Montserrat Llavanera, Marc Bonet, Sergi Fernández Novell, Josep Maria Yeste Oliveras, Marc Rodríguez Gil, Joan Enric 2020 Red LED light acts on the mitochondrial electron chain of mammalian sperm via light-time exposure-dependent mechanisms Cells 9 12 2546reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)Inglésinfo:eu-repo/semantics/altIdentifier/doi/10.3390/Cells9122546info:eu-repo/semantics/altIdentifier/issn/2073-4409info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-88329-Rinfo:eu-repo/grantAgreement/MINECO//RYC-2014-15581Reconeixement 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessoai:recercat.cat:10256/187332026-05-29T05:05:01Z
dc.title.none.fl_str_mv Red LED light acts on the mitochondrial electron chain of mammalian sperm via light-time exposure-dependent mechanisms
title Red LED light acts on the mitochondrial electron chain of mammalian sperm via light-time exposure-dependent mechanisms
spellingShingle Red LED light acts on the mitochondrial electron chain of mammalian sperm via light-time exposure-dependent mechanisms
Blanco Prieto, Olga
Espermatozoides
Mitocondris
Spermatozoa
Mitochondria
Citocrom c
Cytochrome c
Porcs -- Espermatozoides
Swine -- Spermatozoa
title_short Red LED light acts on the mitochondrial electron chain of mammalian sperm via light-time exposure-dependent mechanisms
title_full Red LED light acts on the mitochondrial electron chain of mammalian sperm via light-time exposure-dependent mechanisms
title_fullStr Red LED light acts on the mitochondrial electron chain of mammalian sperm via light-time exposure-dependent mechanisms
title_full_unstemmed Red LED light acts on the mitochondrial electron chain of mammalian sperm via light-time exposure-dependent mechanisms
title_sort Red LED light acts on the mitochondrial electron chain of mammalian sperm via light-time exposure-dependent mechanisms
dc.creator.none.fl_str_mv Blanco Prieto, Olga
Catalán, Jaime
Trujillo Rojas, Lina
Peña García, Àlex
Rivera del Álamo, Ma. Montserrat
Llavanera, Marc
Bonet, Sergi
Fernández Novell, Josep Maria
Yeste Oliveras, Marc
Rodríguez Gil, Joan Enric
author Blanco Prieto, Olga
author_facet Blanco Prieto, Olga
Catalán, Jaime
Trujillo Rojas, Lina
Peña García, Àlex
Rivera del Álamo, Ma. Montserrat
Llavanera, Marc
Bonet, Sergi
Fernández Novell, Josep Maria
Yeste Oliveras, Marc
Rodríguez Gil, Joan Enric
author_role author
author2 Catalán, Jaime
Trujillo Rojas, Lina
Peña García, Àlex
Rivera del Álamo, Ma. Montserrat
Llavanera, Marc
Bonet, Sergi
Fernández Novell, Josep Maria
Yeste Oliveras, Marc
Rodríguez Gil, Joan Enric
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Agencia Estatal de Investigación
Ministerio de Economía y Competitividad (Espanya)
dc.subject.none.fl_str_mv Espermatozoides
Mitocondris
Spermatozoa
Mitochondria
Citocrom c
Cytochrome c
Porcs -- Espermatozoides
Swine -- Spermatozoa
topic Espermatozoides
Mitocondris
Spermatozoa
Mitochondria
Citocrom c
Cytochrome c
Porcs -- Espermatozoides
Swine -- Spermatozoa
description This work analyzes the effects of red LED light on mammalian sperm mitochondrial function, using the pig as an animal model. Liquid-stored pig semen was stimulated with red-light for 1, 5 and 10 min in the presence or absence of oligomycin A, a specific inhibitor of mitochondrial ATP synthase, or carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone (FCCP), a specific disruptor of mitochondrial electron chain. Whereas exposure for 1 and 5 min significantly (p < 0.05) decreased total motility and intracellular ATP levels, irradiation for 10 min induced the opposite effect. Oligomycin A abolished the light-effects on intracellular ATP levels, O2 consumption and mitochondrial membrane potential, whereas compared to non-irradiated samples, FCCP significantly (p < 0.05) increased O2 consumption when sperm were irradiated for 1 min. Both oligomycin A and FCCP significantly (p < 0.05) decreased total motility. Red-light increased cytochrome c oxidase activity with a maximal effect after 5 min of irradiation, which was abolished by both oligomycin A and FCCP. In conclusion, red-light modulates sperm mitochondrial function via electron chain activity in an exposition, time-dependent manner
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
peer-reviewed
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10256/18733
http://hdl.handle.net/10256/18733
url http://hdl.handle.net/10256/18733
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3390/Cells9122546
info:eu-repo/semantics/altIdentifier/issn/2073-4409
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-88329-R
info:eu-repo/grantAgreement/MINECO//RYC-2014-15581
dc.rights.none.fl_str_mv Reconeixement 4.0 Internacional
http://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Reconeixement 4.0 Internacional
http://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Cells, 2020, vol. 9, núm. 12, p. 2546
Articles publicats (D-B)
Blanco Prieto, Olga Catalán, Jaime Trujillo Rojas, Lina Peña García, Àlex Rivera del Álamo, Ma. Montserrat Llavanera, Marc Bonet, Sergi Fernández Novell, Josep Maria Yeste Oliveras, Marc Rodríguez Gil, Joan Enric 2020 Red LED light acts on the mitochondrial electron chain of mammalian sperm via light-time exposure-dependent mechanisms Cells 9 12 2546
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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