Exosomes derived from HEK293T cells interact in an efficient and non-invasive manner with mammalian sperm in vitro
Aims: To investigate exosomes as a non-invasive delivery tool for mammalian sperm. Materials & Methods: Exosomes were isolated from HEK293T cells and co-incubated with boar sperm in vitro. Results: Internalized exosomes were detected within 10 minutes of co-incubation. Computer-assisted sperm an...
| Autores: | , , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2020 |
| País: | España |
| Institución: | Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| Repositorio: | Recercat. Dipósit de la Recerca de Catalunya |
| OAI Identifier: | oai:recercat.cat:10256/18417 |
| Acceso en línea: | http://hdl.handle.net/10256/18417 |
| Access Level: | acceso abierto |
| Palabra clave: | Porcs -- Espermatozoides -- Investigació Swine -- Spermatozoa -- Research Mitocondris Mitochondria |
| Sumario: | Aims: To investigate exosomes as a non-invasive delivery tool for mammalian sperm. Materials & Methods: Exosomes were isolated from HEK293T cells and co-incubated with boar sperm in vitro. Results: Internalized exosomes were detected within 10 minutes of co-incubation. Computer-assisted sperm analysis and flow cytometry demonstrated that even after 5-hours of exposure to exosomes, there were no significant deleterious effects with regards to sperm motility, viability, membrane integrity and mitochondrial membrane potential (P>0.05), thus indicating that exosomes did not interfere with basic sperm function. Conclusion: HEK293T-derived exosomes interacted with boar sperm without affecting sperm function. Exosomes represent a versatile and promising research tool for studying sperm biology and provide new options for the diagnosis and treatment of male infertility |
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