Oocyte morphometric assessment and gene expression profiling of oocytes and cumulus cells as biomarkers of oocyte competence in sheep

This article belongs to the Special Issue In Vitro Embryo Production in Domestic Animals.

Bibliographic Details
Authors: Maside, Carolina, Sánchez-Ajofrín, Irene, Medina-Chávez, Daniela-Alejandra, Alves, Benner, Garde, José Julián, Soler, Ana J.
Format: article
Status:Published version
Publication Date:2021
Country:España
Institution:Consejo Superior de Investigaciones Científicas (CSIC)
Repository:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/259607
Online Access:http://hdl.handle.net/10261/259607
Access Level:Open access
Keyword:Biomarkers
Sheep
Oocyte quality
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spelling Oocyte morphometric assessment and gene expression profiling of oocytes and cumulus cells as biomarkers of oocyte competence in sheepMaside, CarolinaSánchez-Ajofrín, IreneMedina-Chávez, Daniela-AlejandraAlves, BennerGarde, José JuliánSoler, Ana J.BiomarkersSheepOocyte qualityThis article belongs to the Special Issue In Vitro Embryo Production in Domestic Animals.Oocyte quality is crucial for subsequent embryo development and so it is a major challenge in assisted reproductive technologies. The aim of the present work was to evaluate the morphometric parameters of oocytes (experiment 1) and the relative gene expression of oocytes and cumulus cells (CCs) (experiment 2) as biomarkers of oocyte quality after individually culturing them (one oocyte or embryo/drop). In experiment 1, individually matured oocytes were measured and classified into small, intermediate, and large oocytes after a cluster analysis, based on total diameter (with zona pellucida, ZP), oocyte diameter (without ZP), and ZP thickness. These oocytes were individually fertilized in vitro and cultured. The embryo development was evaluated up to the blastocyst stage. According to the total diameter, oocyte diameter, and ZP thickness, the blastocyst rate decreased in the small oocytes group (3.1 ± 3.1, 14.1 ± 9.4, and 26.7 ± 3.9, respectively) compared to the intermediate (29.4 ± 5.2, 30.5 ± 10.1, and 28.6 ± 9.6, respectively) and large oocytes groups (54.2 ± 13.5, 44.4 ± 3.9, and 67.6 ± 12.4, respectively). In addition, the probability of reaching the blastocyst stage was positively related to the total diameter (p < 0.001), oocyte diameter (p < 0.05), and ZP thickness (p < 0.001). Furthermore, the relative gene expression of BAX, BCL2, GDF9, and GJA1 was lower in oocytes classified as large. In experiment 2, the mRNA transcript relative abundance pattern of genes in CCs was evaluated according to oocyte total diameter and developmental stage reached. CCs from oocytes classified as large and oocytes capable of developing to the blastocyst stage had a lower relative expression of BAX, STAR, and PTGS2, while a higher expression of HAS2 and SDC2 transcript was observed for those oocytes. In conclusion, oocyte morphometric parameters and gene expression analysis in oocytes and CCs provide methods for the identification of the most competent oocytes for assisted reproductive technologies in sheep.This research was funded by the Spanish Ministry of Economy and Competitiveness (AGL2017-89017-R); The Junta de Comunidades de Castilla-La Mancha, Spain (PRT program; SBPLY/17/180501/000500), which provided co-funding support to C.M.Peer reviewedMultidisciplinary Digital Publishing InstituteMinisterio de Economía y Competitividad (España)Agencia Estatal de Investigación (España)Junta de Comunidades de Castilla-La ManchaMinisterio de Ciencia, Innovación y Universidades (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202220222021info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/259607reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-89017-Rhttps://doi.org/10.3390/ani11102818Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2596072026-05-22T06:33:51Z
dc.title.none.fl_str_mv Oocyte morphometric assessment and gene expression profiling of oocytes and cumulus cells as biomarkers of oocyte competence in sheep
title Oocyte morphometric assessment and gene expression profiling of oocytes and cumulus cells as biomarkers of oocyte competence in sheep
spellingShingle Oocyte morphometric assessment and gene expression profiling of oocytes and cumulus cells as biomarkers of oocyte competence in sheep
Maside, Carolina
Biomarkers
Sheep
Oocyte quality
title_short Oocyte morphometric assessment and gene expression profiling of oocytes and cumulus cells as biomarkers of oocyte competence in sheep
title_full Oocyte morphometric assessment and gene expression profiling of oocytes and cumulus cells as biomarkers of oocyte competence in sheep
title_fullStr Oocyte morphometric assessment and gene expression profiling of oocytes and cumulus cells as biomarkers of oocyte competence in sheep
title_full_unstemmed Oocyte morphometric assessment and gene expression profiling of oocytes and cumulus cells as biomarkers of oocyte competence in sheep
title_sort Oocyte morphometric assessment and gene expression profiling of oocytes and cumulus cells as biomarkers of oocyte competence in sheep
dc.creator.none.fl_str_mv Maside, Carolina
Sánchez-Ajofrín, Irene
Medina-Chávez, Daniela-Alejandra
Alves, Benner
Garde, José Julián
Soler, Ana J.
author Maside, Carolina
author_facet Maside, Carolina
Sánchez-Ajofrín, Irene
Medina-Chávez, Daniela-Alejandra
Alves, Benner
Garde, José Julián
Soler, Ana J.
author_role author
author2 Sánchez-Ajofrín, Irene
Medina-Chávez, Daniela-Alejandra
Alves, Benner
Garde, José Julián
Soler, Ana J.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Agencia Estatal de Investigación (España)
Junta de Comunidades de Castilla-La Mancha
Ministerio de Ciencia, Innovación y Universidades (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Biomarkers
Sheep
Oocyte quality
topic Biomarkers
Sheep
Oocyte quality
description This article belongs to the Special Issue In Vitro Embryo Production in Domestic Animals.
publishDate 2021
dc.date.none.fl_str_mv 2021
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/259607
url http://hdl.handle.net/10261/259607
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-89017-R
https://doi.org/10.3390/ani11102818
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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