Assessing Autophagy in Archived Tissue or How to Capture Autophagic Flux from a Tissue Snapshot

Autophagy is a highly conserved degradation mechanism that is essential for maintaining cellular homeostasis. In human disease, autophagy pathways are frequently deregulated and there is immense interest in targeting autophagy for therapeutic approaches. Accordingly, there is a need to determine aut...

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Detalles Bibliográficos
Autores: Humbert, Magali, Morán, María, de la Cruz-Ojeda, Patricia, Muntané, Jordi, Wiedmer, Tabea, Apostolova, Nadezda, McKenna, Sharon L., Velasco Díez, Guillermo, Balduini, Walter, Eckhart, Leopold, Janji, Bassam, Sampaio-Marques, Belém, Ludovico, Paula, Žerovnik, Eva, Langer, Rupert, Perren, Aurel, Engedal, Nikolai, Tschan, Mario P.
Tipo de recurso: artículo
Fecha de publicación:2020
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/7900
Acceso en línea:https://hdl.handle.net/20.500.14352/7900
Access Level:acceso abierto
Palabra clave:autophagy
biomarkers
pathology
disease
Biología molecular (Biología)
Bioquímica (Biología)
2415 Biología Molecular
2302 Bioquímica
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oai_identifier_str oai:docta.ucm.es:20.500.14352/7900
network_acronym_str ES
network_name_str España
repository_id_str
spelling Assessing Autophagy in Archived Tissue or How to Capture Autophagic Flux from a Tissue SnapshotHumbert, MagaliMorán, Maríade la Cruz-Ojeda, PatriciaMuntané, JordiWiedmer, TabeaApostolova, NadezdaMcKenna, Sharon L.Velasco Díez, GuillermoBalduini, WalterEckhart, LeopoldJanji, BassamSampaio-Marques, BelémLudovico, PaulaŽerovnik, EvaLanger, RupertPerren, AurelEngedal, NikolaiTschan, Mario P.autophagybiomarkerspathologydiseaseBiología molecular (Biología)Bioquímica (Biología)2415 Biología Molecular2302 BioquímicaAutophagy is a highly conserved degradation mechanism that is essential for maintaining cellular homeostasis. In human disease, autophagy pathways are frequently deregulated and there is immense interest in targeting autophagy for therapeutic approaches. Accordingly, there is a need to determine autophagic activity in human tissues, an endeavor that is hampered by the fact that autophagy is characterized by the flux of substrates whereas histology informs only about amounts and localization of substrates and regulators at a single timepoint. Despite this challenging task, considerable progress in establishing markers of autophagy has been made in recent years. The importance of establishing clear-cut autophagy markers that can be used for tissue analysis cannot be underestimated. In this review, we attempt to summarize known techniques to quantify autophagy in human tissue and their drawbacks. Furthermore, we provide some recommendations that should be taken into consideration to improve the reliability and the interpretation of autophagy biomarkers in human tissue samples.MDPIUniversidad Complutense de Madrid20202020-03-2120202020-03-21journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/7900reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Atribución 3.0 Españahttps://creativecommons.org/licenses/by/3.0/es/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/79002026-06-02T12:44:21Z
dc.title.none.fl_str_mv Assessing Autophagy in Archived Tissue or How to Capture Autophagic Flux from a Tissue Snapshot
title Assessing Autophagy in Archived Tissue or How to Capture Autophagic Flux from a Tissue Snapshot
spellingShingle Assessing Autophagy in Archived Tissue or How to Capture Autophagic Flux from a Tissue Snapshot
Humbert, Magali
autophagy
biomarkers
pathology
disease
Biología molecular (Biología)
Bioquímica (Biología)
2415 Biología Molecular
2302 Bioquímica
title_short Assessing Autophagy in Archived Tissue or How to Capture Autophagic Flux from a Tissue Snapshot
title_full Assessing Autophagy in Archived Tissue or How to Capture Autophagic Flux from a Tissue Snapshot
title_fullStr Assessing Autophagy in Archived Tissue or How to Capture Autophagic Flux from a Tissue Snapshot
title_full_unstemmed Assessing Autophagy in Archived Tissue or How to Capture Autophagic Flux from a Tissue Snapshot
title_sort Assessing Autophagy in Archived Tissue or How to Capture Autophagic Flux from a Tissue Snapshot
dc.creator.none.fl_str_mv Humbert, Magali
Morán, María
de la Cruz-Ojeda, Patricia
Muntané, Jordi
Wiedmer, Tabea
Apostolova, Nadezda
McKenna, Sharon L.
Velasco Díez, Guillermo
Balduini, Walter
Eckhart, Leopold
Janji, Bassam
Sampaio-Marques, Belém
Ludovico, Paula
Žerovnik, Eva
Langer, Rupert
Perren, Aurel
Engedal, Nikolai
Tschan, Mario P.
author Humbert, Magali
author_facet Humbert, Magali
Morán, María
de la Cruz-Ojeda, Patricia
Muntané, Jordi
Wiedmer, Tabea
Apostolova, Nadezda
McKenna, Sharon L.
Velasco Díez, Guillermo
Balduini, Walter
Eckhart, Leopold
Janji, Bassam
Sampaio-Marques, Belém
Ludovico, Paula
Žerovnik, Eva
Langer, Rupert
Perren, Aurel
Engedal, Nikolai
Tschan, Mario P.
author_role author
author2 Morán, María
de la Cruz-Ojeda, Patricia
Muntané, Jordi
Wiedmer, Tabea
Apostolova, Nadezda
McKenna, Sharon L.
Velasco Díez, Guillermo
Balduini, Walter
Eckhart, Leopold
Janji, Bassam
Sampaio-Marques, Belém
Ludovico, Paula
Žerovnik, Eva
Langer, Rupert
Perren, Aurel
Engedal, Nikolai
Tschan, Mario P.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv autophagy
biomarkers
pathology
disease
Biología molecular (Biología)
Bioquímica (Biología)
2415 Biología Molecular
2302 Bioquímica
topic autophagy
biomarkers
pathology
disease
Biología molecular (Biología)
Bioquímica (Biología)
2415 Biología Molecular
2302 Bioquímica
description Autophagy is a highly conserved degradation mechanism that is essential for maintaining cellular homeostasis. In human disease, autophagy pathways are frequently deregulated and there is immense interest in targeting autophagy for therapeutic approaches. Accordingly, there is a need to determine autophagic activity in human tissues, an endeavor that is hampered by the fact that autophagy is characterized by the flux of substrates whereas histology informs only about amounts and localization of substrates and regulators at a single timepoint. Despite this challenging task, considerable progress in establishing markers of autophagy has been made in recent years. The importance of establishing clear-cut autophagy markers that can be used for tissue analysis cannot be underestimated. In this review, we attempt to summarize known techniques to quantify autophagy in human tissue and their drawbacks. Furthermore, we provide some recommendations that should be taken into consideration to improve the reliability and the interpretation of autophagy biomarkers in human tissue samples.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020-03-21
2020
2020-03-21
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/7900
url https://hdl.handle.net/20.500.14352/7900
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 3.0 España
https://creativecommons.org/licenses/by/3.0/es/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 3.0 España
https://creativecommons.org/licenses/by/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,301629

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