Assessing Autophagy in Archived Tissue or How to Capture Autophagic Flux from a Tissue Snapshot

Autophagy is a highly conserved degradation mechanism that is essential for maintaining cellular homeostasis. In human disease, autophagy pathways are frequently deregulated and there is immense interest in targeting autophagy for therapeutic approaches. Accordingly, there is a need to determine aut...

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Detalles Bibliográficos
Autores: Humbert, Magali, Morán, María, de la Cruz-Ojeda, Patricia, Muntané, Jordi, Wiedmer, Tabea, Apostolova, Nadezda, McKenna, Sharon L., Velasco Díez, Guillermo, Balduini, Walter, Eckhart, Leopold, Janji, Bassam, Sampaio-Marques, Belém, Ludovico, Paula, Žerovnik, Eva, Langer, Rupert, Perren, Aurel, Engedal, Nikolai, Tschan, Mario P.
Tipo de recurso: artículo
Fecha de publicación:2020
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/7900
Acceso en línea:https://hdl.handle.net/20.500.14352/7900
Access Level:acceso abierto
Palabra clave:autophagy
biomarkers
pathology
disease
Biología molecular (Biología)
Bioquímica (Biología)
2415 Biología Molecular
2302 Bioquímica
Descripción
Sumario:Autophagy is a highly conserved degradation mechanism that is essential for maintaining cellular homeostasis. In human disease, autophagy pathways are frequently deregulated and there is immense interest in targeting autophagy for therapeutic approaches. Accordingly, there is a need to determine autophagic activity in human tissues, an endeavor that is hampered by the fact that autophagy is characterized by the flux of substrates whereas histology informs only about amounts and localization of substrates and regulators at a single timepoint. Despite this challenging task, considerable progress in establishing markers of autophagy has been made in recent years. The importance of establishing clear-cut autophagy markers that can be used for tissue analysis cannot be underestimated. In this review, we attempt to summarize known techniques to quantify autophagy in human tissue and their drawbacks. Furthermore, we provide some recommendations that should be taken into consideration to improve the reliability and the interpretation of autophagy biomarkers in human tissue samples.

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