Electrochemical immunosensor for simultaneous determination of interleukin-1 beta and tumor necrosis factor alpha in serum and saliva using dual screen printed electrodes modified with functionalized doubleewalled carbon nanotubes

Dual screen-printed carbon electrodes modified with 4-carboxyphenyl-functionalized double-walled carbon nanotubes (HOOC-Phe-DWCNTs/SPCEs) have been used as scaffolds for the preparation of electrochemical immunosensors for the simultaneous determination of the cytokines Interleukin-1b (IL-1b) and fa...

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Detalhes bibliográficos
Autores: Sánchez Tirado, Esther, Salvo, Coral, González Cortés, Araceli, Yáñez-Sedeño Orive, Paloma, Langa, Fernando, Pingarrón Carrazón, José Manuel
Formato: artículo
Fecha de publicación:2017
País:España
Recursos:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/18769
Acesso em linha:https://hdl.handle.net/20.500.14352/18769
Access Level:acceso abierto
Palavra-chave:543
Interleukin-1 beta
Tumor necrosis factor-alpha
Electrochemical immunosensor
Multiplex
Double-walled carbon nanotubes
Saliva
Química analítica (Química)
2301 Química Analítica
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oai_identifier_str oai:docta.ucm.es:20.500.14352/18769
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network_name_str España
repository_id_str
spelling Electrochemical immunosensor for simultaneous determination of interleukin-1 beta and tumor necrosis factor alpha in serum and saliva using dual screen printed electrodes modified with functionalized doubleewalled carbon nanotubesSánchez Tirado, EstherSalvo, CoralGonzález Cortés, AraceliYáñez-Sedeño Orive, PalomaLanga, FernandoPingarrón Carrazón, José Manuel543Interleukin-1 betaTumor necrosis factor-alphaElectrochemical immunosensorMultiplexDouble-walled carbon nanotubesSalivaQuímica analítica (Química)2301 Química AnalíticaDual screen-printed carbon electrodes modified with 4-carboxyphenyl-functionalized double-walled carbon nanotubes (HOOC-Phe-DWCNTs/SPCEs) have been used as scaffolds for the preparation of electrochemical immunosensors for the simultaneous determination of the cytokines Interleukin-1b (IL-1b) and factor necrosis tumor a (TNF-a). IL-1b. Capture antibodies were immobilized onto HOOC-PheDWCNTs/SPCEs in an oriented form making using the commercial polymeric coating Mix&Go™. Sandwich type immunoassays with amperometric signal amplification through the use of poly-HRPstreptavidin conjugates and H2O2 as HRP substrate and hydroquinone as redox mediator were implemented. Upon optimization of the experimental variables affecting the immunosensor performance, the dual immunosensor allows ranges of linearity extending between 0.5 and 100 pg/mL and from 1 to 200 pg/mL for IL-1b and TNF-a, respectively, these ranges being adequate for the determination of the cytokines in clinical samples. The achieved limits of detection were 0.38 pg/mL (IL-1b) and 0.85 pg/mL (TNF-a). In addition, the dual immunosensor exhibits excellent reproducibility of the measurements, storage stability of the anti-IL-Phe-DWCNTs/SPCE and anti-TNF-Phe-DWCNTs/SPCE conjugates, and selectivity as well as negligible cross-talking. The dual immunosensor was applied to the simultaneous determination of IL-1b and TNF-a in human serum spiked at clinically relevant concentration levels and in real saliva samples.ElsevierUniversidad Complutense de Madrid20172017-03-0120172017-03-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/18769reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)InglésengMinisterio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 CTQ2015-70023-R Not availableMinisterio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 CTQ2015-71955-REDT Not availableComunidad de Madrid NANOAVANSENS S2013%MT-3029open accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/187692026-06-02T12:44:21Z
dc.title.none.fl_str_mv Electrochemical immunosensor for simultaneous determination of interleukin-1 beta and tumor necrosis factor alpha in serum and saliva using dual screen printed electrodes modified with functionalized doubleewalled carbon nanotubes
title Electrochemical immunosensor for simultaneous determination of interleukin-1 beta and tumor necrosis factor alpha in serum and saliva using dual screen printed electrodes modified with functionalized doubleewalled carbon nanotubes
spellingShingle Electrochemical immunosensor for simultaneous determination of interleukin-1 beta and tumor necrosis factor alpha in serum and saliva using dual screen printed electrodes modified with functionalized doubleewalled carbon nanotubes
Sánchez Tirado, Esther
543
Interleukin-1 beta
Tumor necrosis factor-alpha
Electrochemical immunosensor
Multiplex
Double-walled carbon nanotubes
Saliva
Química analítica (Química)
2301 Química Analítica
title_short Electrochemical immunosensor for simultaneous determination of interleukin-1 beta and tumor necrosis factor alpha in serum and saliva using dual screen printed electrodes modified with functionalized doubleewalled carbon nanotubes
title_full Electrochemical immunosensor for simultaneous determination of interleukin-1 beta and tumor necrosis factor alpha in serum and saliva using dual screen printed electrodes modified with functionalized doubleewalled carbon nanotubes
title_fullStr Electrochemical immunosensor for simultaneous determination of interleukin-1 beta and tumor necrosis factor alpha in serum and saliva using dual screen printed electrodes modified with functionalized doubleewalled carbon nanotubes
title_full_unstemmed Electrochemical immunosensor for simultaneous determination of interleukin-1 beta and tumor necrosis factor alpha in serum and saliva using dual screen printed electrodes modified with functionalized doubleewalled carbon nanotubes
title_sort Electrochemical immunosensor for simultaneous determination of interleukin-1 beta and tumor necrosis factor alpha in serum and saliva using dual screen printed electrodes modified with functionalized doubleewalled carbon nanotubes
dc.creator.none.fl_str_mv Sánchez Tirado, Esther
Salvo, Coral
González Cortés, Araceli
Yáñez-Sedeño Orive, Paloma
Langa, Fernando
Pingarrón Carrazón, José Manuel
author Sánchez Tirado, Esther
author_facet Sánchez Tirado, Esther
Salvo, Coral
González Cortés, Araceli
Yáñez-Sedeño Orive, Paloma
Langa, Fernando
Pingarrón Carrazón, José Manuel
author_role author
author2 Salvo, Coral
González Cortés, Araceli
Yáñez-Sedeño Orive, Paloma
Langa, Fernando
Pingarrón Carrazón, José Manuel
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 543
Interleukin-1 beta
Tumor necrosis factor-alpha
Electrochemical immunosensor
Multiplex
Double-walled carbon nanotubes
Saliva
Química analítica (Química)
2301 Química Analítica
topic 543
Interleukin-1 beta
Tumor necrosis factor-alpha
Electrochemical immunosensor
Multiplex
Double-walled carbon nanotubes
Saliva
Química analítica (Química)
2301 Química Analítica
description Dual screen-printed carbon electrodes modified with 4-carboxyphenyl-functionalized double-walled carbon nanotubes (HOOC-Phe-DWCNTs/SPCEs) have been used as scaffolds for the preparation of electrochemical immunosensors for the simultaneous determination of the cytokines Interleukin-1b (IL-1b) and factor necrosis tumor a (TNF-a). IL-1b. Capture antibodies were immobilized onto HOOC-PheDWCNTs/SPCEs in an oriented form making using the commercial polymeric coating Mix&Go™. Sandwich type immunoassays with amperometric signal amplification through the use of poly-HRPstreptavidin conjugates and H2O2 as HRP substrate and hydroquinone as redox mediator were implemented. Upon optimization of the experimental variables affecting the immunosensor performance, the dual immunosensor allows ranges of linearity extending between 0.5 and 100 pg/mL and from 1 to 200 pg/mL for IL-1b and TNF-a, respectively, these ranges being adequate for the determination of the cytokines in clinical samples. The achieved limits of detection were 0.38 pg/mL (IL-1b) and 0.85 pg/mL (TNF-a). In addition, the dual immunosensor exhibits excellent reproducibility of the measurements, storage stability of the anti-IL-Phe-DWCNTs/SPCE and anti-TNF-Phe-DWCNTs/SPCE conjugates, and selectivity as well as negligible cross-talking. The dual immunosensor was applied to the simultaneous determination of IL-1b and TNF-a in human serum spiked at clinically relevant concentration levels and in real saliva samples.
publishDate 2017
dc.date.none.fl_str_mv 2017
2017-03-01
2017
2017-03-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/18769
url https://hdl.handle.net/20.500.14352/18769
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Ministerio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 CTQ2015-70023-R Not available
Ministerio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 CTQ2015-71955-REDT Not available
Comunidad de Madrid NANOAVANSENS S2013%MT-3029
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869405173371109376
score 15,301603

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