Effect of soy isoflavones on growth of representative bacterial species from the human gut
The present work aimed to assess the susceptibility of dominant and representative bacterial populations from the human gut to isoflavones and their metabolites. To do so, the minimum inhibitory concentration (MIC) of isoflavone glycosides, isoflavone aglycones, and equol to 37 bacterial strains was...
| Autores: | , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2017 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/185137 |
| Acceso en línea: | http://hdl.handle.net/10261/185137 |
| Access Level: | acceso abierto |
| Palabra clave: | Isoflavones Daidzein Genistein Equol Minimum inhibitory concentration Lactic acid bacteria Bifidobacteria Intestinal bacteria |
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Effect of soy isoflavones on growth of representative bacterial species from the human gutVázquez, LucíaFlórez, Ana BelénGuadamuro, LucíaMayo Pérez, BaltasarIsoflavonesDaidzeinGenisteinEquolMinimum inhibitory concentrationLactic acid bacteriaBifidobacteriaIntestinal bacteriaThe present work aimed to assess the susceptibility of dominant and representative bacterial populations from the human gut to isoflavones and their metabolites. To do so, the minimum inhibitory concentration (MIC) of isoflavone glycosides, isoflavone aglycones, and equol to 37 bacterial strains was determined by broth microdilution. Additionally, for 10 representative strains, growth curves, growth rate (μ), and optical density (OD600 nm) of the cultures at 24 h were also determined. MICs of daidzin, genistin, daidzein, and genistein were >2048 μg mL−1 for all strains assayed, while that of equol ranged from 16 μg mL−1 for Bifidobacterium animalis subsp. animalis to >2048 μg mL−1 for Enterobacteriaceae strains. Changes in growth curves, μ, and final OD were observed among the species in the presence of all tested compounds. Genistein reduced μ of Bacteroides fragilis, Lactococcus lactis subsp. lactis, and Slackia equolifaciens, while both genistein and equol increased that of Lactobacillus rhamnosus and Faecalibacterium prausnitzii. Compared to controls, lower final OD in the presence of aglycones and equol were recorded for some strains but were higher for others. Altogether, the results suggest that isoflavone-derived compounds could modify numbers of key bacterial species in the gut, which might be associated with their beneficial propertiesThe study was partially supported by projects from the Spanish Ministry of Economy and Competitiveness (AGL-2014-57820-R) and Asturias Principality (GRUPIN14-137). L.G. and L.V. were supported by research contracts of the FPI Program from MINECO (BES-2012-062502 and BES-2015-072285, respectively).Peer reviewedMolecular Diversity Preservation InternationalMinisterio de Economía y Competitividad (España)Principado de AsturiasMayo Pérez, Baltasar [0000-0001-5634-6543]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201920192017info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/185137reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL-2014-57820-Rhttp://dx.doi.org/10.3390/nu9070727Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1851372026-05-22T06:33:51Z |
| dc.title.none.fl_str_mv |
Effect of soy isoflavones on growth of representative bacterial species from the human gut |
| title |
Effect of soy isoflavones on growth of representative bacterial species from the human gut |
| spellingShingle |
Effect of soy isoflavones on growth of representative bacterial species from the human gut Vázquez, Lucía Isoflavones Daidzein Genistein Equol Minimum inhibitory concentration Lactic acid bacteria Bifidobacteria Intestinal bacteria |
| title_short |
Effect of soy isoflavones on growth of representative bacterial species from the human gut |
| title_full |
Effect of soy isoflavones on growth of representative bacterial species from the human gut |
| title_fullStr |
Effect of soy isoflavones on growth of representative bacterial species from the human gut |
| title_full_unstemmed |
Effect of soy isoflavones on growth of representative bacterial species from the human gut |
| title_sort |
Effect of soy isoflavones on growth of representative bacterial species from the human gut |
| dc.creator.none.fl_str_mv |
Vázquez, Lucía Flórez, Ana Belén Guadamuro, Lucía Mayo Pérez, Baltasar |
| author |
Vázquez, Lucía |
| author_facet |
Vázquez, Lucía Flórez, Ana Belén Guadamuro, Lucía Mayo Pérez, Baltasar |
| author_role |
author |
| author2 |
Flórez, Ana Belén Guadamuro, Lucía Mayo Pérez, Baltasar |
| author2_role |
author author author |
| dc.contributor.none.fl_str_mv |
Ministerio de Economía y Competitividad (España) Principado de Asturias Mayo Pérez, Baltasar [0000-0001-5634-6543] Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72] |
| dc.subject.none.fl_str_mv |
Isoflavones Daidzein Genistein Equol Minimum inhibitory concentration Lactic acid bacteria Bifidobacteria Intestinal bacteria |
| topic |
Isoflavones Daidzein Genistein Equol Minimum inhibitory concentration Lactic acid bacteria Bifidobacteria Intestinal bacteria |
| description |
The present work aimed to assess the susceptibility of dominant and representative bacterial populations from the human gut to isoflavones and their metabolites. To do so, the minimum inhibitory concentration (MIC) of isoflavone glycosides, isoflavone aglycones, and equol to 37 bacterial strains was determined by broth microdilution. Additionally, for 10 representative strains, growth curves, growth rate (μ), and optical density (OD600 nm) of the cultures at 24 h were also determined. MICs of daidzin, genistin, daidzein, and genistein were >2048 μg mL−1 for all strains assayed, while that of equol ranged from 16 μg mL−1 for Bifidobacterium animalis subsp. animalis to >2048 μg mL−1 for Enterobacteriaceae strains. Changes in growth curves, μ, and final OD were observed among the species in the presence of all tested compounds. Genistein reduced μ of Bacteroides fragilis, Lactococcus lactis subsp. lactis, and Slackia equolifaciens, while both genistein and equol increased that of Lactobacillus rhamnosus and Faecalibacterium prausnitzii. Compared to controls, lower final OD in the presence of aglycones and equol were recorded for some strains but were higher for others. Altogether, the results suggest that isoflavone-derived compounds could modify numbers of key bacterial species in the gut, which might be associated with their beneficial properties |
| publishDate |
2017 |
| dc.date.none.fl_str_mv |
2017 2019 2019 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article http://purl.org/coar/resource_type/c_6501 Publisher's version info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/10261/185137 |
| url |
http://hdl.handle.net/10261/185137 |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
| dc.relation.none.fl_str_mv |
#PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL-2014-57820-R http://dx.doi.org/10.3390/nu9070727 Sí |
| dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.publisher.none.fl_str_mv |
Molecular Diversity Preservation International |
| publisher.none.fl_str_mv |
Molecular Diversity Preservation International |
| dc.source.none.fl_str_mv |
reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC instname:Consejo Superior de Investigaciones Científicas (CSIC) |
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Consejo Superior de Investigaciones Científicas (CSIC) |
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DIGITAL.CSIC. Repositorio Institucional del CSIC |
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DIGITAL.CSIC. Repositorio Institucional del CSIC |
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1869405135171485696 |
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15.811543 |