Novel adsorptive polyamine coating for enhanced capillaryelectrophoresis of basic proteins and peptides

In capillary electrophoresis (CE), the anionic and hydrophobic nature of the fused-silica capillary surface has long been known to present a problem in protein and peptide analysis. The use of capillary surface coating is one of the approaches to avoid the analyte¿wall interactions. In this study, a...

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Detalles Bibliográficos
Autores: Puerta, Angel de la, Axen, Jakob, Soderberg, Lennart, Bergquist, Jonas
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2006
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/346592
Acceso en línea:http://hdl.handle.net/10261/346592
Access Level:acceso abierto
Palabra clave:Capillary electrophoresis
Polyamine coating
Coated capillaries
Proteins
Peptides
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spelling Novel adsorptive polyamine coating for enhanced capillaryelectrophoresis of basic proteins and peptidesPuerta, Angel de laAxen, JakobSoderberg, LennartBergquist, JonasCapillary electrophoresisPolyamine coatingCoated capillariesProteinsPeptidesIn capillary electrophoresis (CE), the anionic and hydrophobic nature of the fused-silica capillary surface has long been known to present a problem in protein and peptide analysis. The use of capillary surface coating is one of the approaches to avoid the analyte¿wall interactions. In this study, a new polymer, poly-LA 313, has been synthesized, physico-chemical characterized, and applied as polyamine coating for CE separations. The coating process is highly reproducible and provides fast separations of peptides and proteins in a few minutes and with high efficiency. The physically adsorbed polymer gives rise to a durable coating in the range of pH 2¿10, in the presence of organic modifiers (acetonitrile and methanol) and with complex biological samples. The efficiency of the new cationic polymer was also tested performing protein and peptide separations with capillary electrophoresis-electrospray ionization-mass spectrometry (CE-ESI-MS).Angel Puerta acknowledges the Swedish Institute for a research grant. The Swedish Research Council is acknowledged for financial support(621-2002-5261,629-2002-6821J.B.).The authors acknowledges Prof. Ulf Hellman for help with the MALDI-MS measurements, G¨ oran Svensk for help with the Light Scattering measurements, Dr. Per Sj¨ oberg for help with the ESI-MS, and Dr. Sara Ullsten for fruitful discussions. Astra Zeneca and Agilent Technologies are acknowledged for the loan of the CE system and the MS system, respectively. Prof. Jonas Bergquist holds a senior research position financed by the Swedish Research Council.Peer reviewedElsevierSwedish InstituteConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2024202420062024info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/346592reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1016/j.jchromb.2006.04.018Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3465922026-05-22T06:33:51Z
dc.title.none.fl_str_mv Novel adsorptive polyamine coating for enhanced capillaryelectrophoresis of basic proteins and peptides
title Novel adsorptive polyamine coating for enhanced capillaryelectrophoresis of basic proteins and peptides
spellingShingle Novel adsorptive polyamine coating for enhanced capillaryelectrophoresis of basic proteins and peptides
Puerta, Angel de la
Capillary electrophoresis
Polyamine coating
Coated capillaries
Proteins
Peptides
title_short Novel adsorptive polyamine coating for enhanced capillaryelectrophoresis of basic proteins and peptides
title_full Novel adsorptive polyamine coating for enhanced capillaryelectrophoresis of basic proteins and peptides
title_fullStr Novel adsorptive polyamine coating for enhanced capillaryelectrophoresis of basic proteins and peptides
title_full_unstemmed Novel adsorptive polyamine coating for enhanced capillaryelectrophoresis of basic proteins and peptides
title_sort Novel adsorptive polyamine coating for enhanced capillaryelectrophoresis of basic proteins and peptides
dc.creator.none.fl_str_mv Puerta, Angel de la
Axen, Jakob
Soderberg, Lennart
Bergquist, Jonas
author Puerta, Angel de la
author_facet Puerta, Angel de la
Axen, Jakob
Soderberg, Lennart
Bergquist, Jonas
author_role author
author2 Axen, Jakob
Soderberg, Lennart
Bergquist, Jonas
author2_role author
author
author
dc.contributor.none.fl_str_mv Swedish Institute
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Capillary electrophoresis
Polyamine coating
Coated capillaries
Proteins
Peptides
topic Capillary electrophoresis
Polyamine coating
Coated capillaries
Proteins
Peptides
description In capillary electrophoresis (CE), the anionic and hydrophobic nature of the fused-silica capillary surface has long been known to present a problem in protein and peptide analysis. The use of capillary surface coating is one of the approaches to avoid the analyte¿wall interactions. In this study, a new polymer, poly-LA 313, has been synthesized, physico-chemical characterized, and applied as polyamine coating for CE separations. The coating process is highly reproducible and provides fast separations of peptides and proteins in a few minutes and with high efficiency. The physically adsorbed polymer gives rise to a durable coating in the range of pH 2¿10, in the presence of organic modifiers (acetonitrile and methanol) and with complex biological samples. The efficiency of the new cationic polymer was also tested performing protein and peptide separations with capillary electrophoresis-electrospray ionization-mass spectrometry (CE-ESI-MS).
publishDate 2006
dc.date.none.fl_str_mv 2006
2024
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/346592
url http://hdl.handle.net/10261/346592
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1016/j.jchromb.2006.04.018
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,198674

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