Potential of the solid-state fermentation of soy fibre residues by native microbial populations for bench-scale alkaline protease production
The production of alkaline proteases by solid-state fermentation (SSF) was evaluated. The effect of three agro-industrial residues was examined: coffee husk, hair waste from the tanning industry and soy fibre residues. Soy fibre presented the highest yield for protease production at the laboratory s...
| Autores: | , |
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| Tipo de recurso: | artículo |
| Fecha de publicación: | 2013 |
| País: | España |
| Institución: | Universitat Autònoma de Barcelona |
| Repositorio: | Dipòsit Digital de Documents de la UAB |
| Idioma: | inglés |
| OAI Identifier: | oai:ddd.uab.cat:196162 |
| Acceso en línea: | https://ddd.uab.cat/record/196162 https://dx.doi.org/urn:doi:10.1016/j.bej.2013.02.008 |
| Access Level: | acceso abierto |
| Palabra clave: | Solid-state fermentation Alkaline protease Respiration activity Experimental design Bench scale production |
| Sumario: | The production of alkaline proteases by solid-state fermentation (SSF) was evaluated. The effect of three agro-industrial residues was examined: coffee husk, hair waste from the tanning industry and soy fibre residues. Soy fibre presented the highest yield for protease production at the laboratory scale (37 °C, 100 g samples). Consequently, experiments with soy fibre (F) and soy fibre with 10% compost as an inoculum (FC) were performed for 14 days in 4.5 L bench-scale aerobic near-adiabatic reactors. The highest activity occurred under thermophilic conditions and a high respiration activity of 47,331 ± 1391 U/g dry matter for F and 18,750 ± 1596 U/g dry matter for FC, which are much higher values than those reported in other studies. Alkaline proteases showed maximum stability at pH 11 and temperatures of 43.8 °C (F) and 30 °C (FC), which was determined by a full factorial experimental design. Storage assays demonstrated that 90% of enzyme activity was preserved for three months by lyophilising or freezing the samples at -80 °C. |
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