Metabolic Effects of Oral Phenelzine Treatment on High-Sucrose-Drinking Mice

Phenelzine has been suggested to have an antiobesity effect by inhibiting de novo lipogenesis, which led us to investigate the metabolic effects of oral chronic phenelzine treatment in high-sucrose-drinking mice. Sucrose-drinking mice presented higher body weight gain and adiposity versus controls....

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Detalles Bibliográficos
Autores: Carpene, Christian, Gomez-Zorita, Saioa, Chaplin, Alice, Mercader, Josep
Tipo de recurso: artículo
Fecha de publicación:2018
País:España
Institución:Instituto de Salud Carlos III (ISCIII)
Repositorio:Repisalud
Idioma:inglés
OAI Identifier:oai:repisalud.isciii.es:20.500.12105/22584
Acceso en línea:https://hdl.handle.net/20.500.12105/22584
Access Level:acceso abierto
Palabra clave:Phenelzine
Obesity
Adipocyte
Lipogenesis
Amine oxidases
Hydrogen peroxide
Hyperglycemia
Oxidative stress
Sacarosa
Aumento de Peso
Animales
Ácidos Grasos no Esterificados
Masculino
Administración Oral
Fenelzina
Tejido Adiposo Blanco
Inhibidores de la Monoaminooxidasa
Obesidad
Ratones Endogámicos C57BL
Estrés Oxidativo
Ratones
Lipogénesis
Adiposidad
Oxidative Stress
Administration, Oral
Mice, Inbred C57BL
Monoamine Oxidase Inhibitors
Male
Fatty Acids, Nonesterified
Adiposity
Animals
Weight Gain
Sucrose
Adipose Tissue, White
Mice
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spelling Metabolic Effects of Oral Phenelzine Treatment on High-Sucrose-Drinking MiceCarpene, ChristianGomez-Zorita, SaioaChaplin, AliceMercader, JosepPhenelzineObesityAdipocyteLipogenesisAmine oxidasesHydrogen peroxideHyperglycemiaOxidative stressSacarosaAumento de PesoAnimalesÁcidos Grasos no EsterificadosMasculinoAdministración OralFenelzinaTejido Adiposo BlancoInhibidores de la MonoaminooxidasaObesidadRatones Endogámicos C57BLEstrés OxidativoRatonesLipogénesisAdiposidadOxidative StressAdministration, OralMice, Inbred C57BLMonoamine Oxidase InhibitorsObesityMaleFatty Acids, NonesterifiedPhenelzineAdiposityAnimalsWeight GainLipogenesisSucroseAdipose Tissue, WhiteMicePhenelzine has been suggested to have an antiobesity effect by inhibiting de novo lipogenesis, which led us to investigate the metabolic effects of oral chronic phenelzine treatment in high-sucrose-drinking mice. Sucrose-drinking mice presented higher body weight gain and adiposity versus controls. Phenelzine addition did not decrease such parameters, even though fat pad lipid content and weights were not different from controls. In visceral adipocytes, phenelzine did not impair insulin-stimulated de novo lipogenesis and had no effect on lipolysis. However, phenelzine reduced the mRNA levels of glucose transporters 1 and 4 and phosphoenolpyruvate carboxykinase in inguinal white adipose tissue (iWAT), and altered circulating levels of free fatty acids (FFA) and glycerol. Interestingly, glycemia was restored in phenelzine-treated mice, which also had higher insulinaemia. Phenelzine-treated mice presented higher rectal temperature, which was associated to reduced mRNA levels of uncoupling protein 1 in brown adipose tissue. Furthermore, unlike sucrose-drinking mice, hepatic malondialdehyde levels were not altered. In conclusion, although de novo lipogenesis was not inhibited by phenelzine, the data suggest that the ability to re-esterify FFA is impaired in iWAT. Moreover, the effects on glucose homeostasis and oxidative stress suggest that phenelzine could alleviate obesity-related alterations and deserves further investigation in obesity models.Multidisciplinary Digital Publishing Institute (MDPI)20242024-09-0620182018-10-0120182018-10-01research articlehttp://purl.org/coar/resource_type/c_2df8fbb1info:eu-repo/semantics/articlehttps://hdl.handle.net/20.500.12105/22584reponame:Repisaludinstname:Instituto de Salud Carlos III (ISCIII)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:repisalud.isciii.es:20.500.12105/225842026-06-12T12:43:37Z
dc.title.none.fl_str_mv Metabolic Effects of Oral Phenelzine Treatment on High-Sucrose-Drinking Mice
title Metabolic Effects of Oral Phenelzine Treatment on High-Sucrose-Drinking Mice
spellingShingle Metabolic Effects of Oral Phenelzine Treatment on High-Sucrose-Drinking Mice
Carpene, Christian
Phenelzine
Obesity
Adipocyte
Lipogenesis
Amine oxidases
Hydrogen peroxide
Hyperglycemia
Oxidative stress
Sacarosa
Aumento de Peso
Animales
Ácidos Grasos no Esterificados
Masculino
Administración Oral
Fenelzina
Tejido Adiposo Blanco
Inhibidores de la Monoaminooxidasa
Obesidad
Ratones Endogámicos C57BL
Estrés Oxidativo
Ratones
Lipogénesis
Adiposidad
Oxidative Stress
Administration, Oral
Mice, Inbred C57BL
Monoamine Oxidase Inhibitors
Obesity
Male
Fatty Acids, Nonesterified
Phenelzine
Adiposity
Animals
Weight Gain
Lipogenesis
Sucrose
Adipose Tissue, White
Mice
title_short Metabolic Effects of Oral Phenelzine Treatment on High-Sucrose-Drinking Mice
title_full Metabolic Effects of Oral Phenelzine Treatment on High-Sucrose-Drinking Mice
title_fullStr Metabolic Effects of Oral Phenelzine Treatment on High-Sucrose-Drinking Mice
title_full_unstemmed Metabolic Effects of Oral Phenelzine Treatment on High-Sucrose-Drinking Mice
title_sort Metabolic Effects of Oral Phenelzine Treatment on High-Sucrose-Drinking Mice
dc.creator.none.fl_str_mv Carpene, Christian
Gomez-Zorita, Saioa
Chaplin, Alice
Mercader, Josep
author Carpene, Christian
author_facet Carpene, Christian
Gomez-Zorita, Saioa
Chaplin, Alice
Mercader, Josep
author_role author
author2 Gomez-Zorita, Saioa
Chaplin, Alice
Mercader, Josep
author2_role author
author
author
dc.contributor.none.fl_str_mv
dc.subject.none.fl_str_mv Phenelzine
Obesity
Adipocyte
Lipogenesis
Amine oxidases
Hydrogen peroxide
Hyperglycemia
Oxidative stress
Sacarosa
Aumento de Peso
Animales
Ácidos Grasos no Esterificados
Masculino
Administración Oral
Fenelzina
Tejido Adiposo Blanco
Inhibidores de la Monoaminooxidasa
Obesidad
Ratones Endogámicos C57BL
Estrés Oxidativo
Ratones
Lipogénesis
Adiposidad
Oxidative Stress
Administration, Oral
Mice, Inbred C57BL
Monoamine Oxidase Inhibitors
Obesity
Male
Fatty Acids, Nonesterified
Phenelzine
Adiposity
Animals
Weight Gain
Lipogenesis
Sucrose
Adipose Tissue, White
Mice
topic Phenelzine
Obesity
Adipocyte
Lipogenesis
Amine oxidases
Hydrogen peroxide
Hyperglycemia
Oxidative stress
Sacarosa
Aumento de Peso
Animales
Ácidos Grasos no Esterificados
Masculino
Administración Oral
Fenelzina
Tejido Adiposo Blanco
Inhibidores de la Monoaminooxidasa
Obesidad
Ratones Endogámicos C57BL
Estrés Oxidativo
Ratones
Lipogénesis
Adiposidad
Oxidative Stress
Administration, Oral
Mice, Inbred C57BL
Monoamine Oxidase Inhibitors
Obesity
Male
Fatty Acids, Nonesterified
Phenelzine
Adiposity
Animals
Weight Gain
Lipogenesis
Sucrose
Adipose Tissue, White
Mice
description Phenelzine has been suggested to have an antiobesity effect by inhibiting de novo lipogenesis, which led us to investigate the metabolic effects of oral chronic phenelzine treatment in high-sucrose-drinking mice. Sucrose-drinking mice presented higher body weight gain and adiposity versus controls. Phenelzine addition did not decrease such parameters, even though fat pad lipid content and weights were not different from controls. In visceral adipocytes, phenelzine did not impair insulin-stimulated de novo lipogenesis and had no effect on lipolysis. However, phenelzine reduced the mRNA levels of glucose transporters 1 and 4 and phosphoenolpyruvate carboxykinase in inguinal white adipose tissue (iWAT), and altered circulating levels of free fatty acids (FFA) and glycerol. Interestingly, glycemia was restored in phenelzine-treated mice, which also had higher insulinaemia. Phenelzine-treated mice presented higher rectal temperature, which was associated to reduced mRNA levels of uncoupling protein 1 in brown adipose tissue. Furthermore, unlike sucrose-drinking mice, hepatic malondialdehyde levels were not altered. In conclusion, although de novo lipogenesis was not inhibited by phenelzine, the data suggest that the ability to re-esterify FFA is impaired in iWAT. Moreover, the effects on glucose homeostasis and oxidative stress suggest that phenelzine could alleviate obesity-related alterations and deserves further investigation in obesity models.
publishDate 2018
dc.date.none.fl_str_mv 2018
2018-10-01
2018
2018-10-01
2024
2024-09-06
dc.type.none.fl_str_mv research article
http://purl.org/coar/resource_type/c_2df8fbb1
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.12105/22584
url https://hdl.handle.net/20.500.12105/22584
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute (MDPI)
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute (MDPI)
dc.source.none.fl_str_mv reponame:Repisalud
instname:Instituto de Salud Carlos III (ISCIII)
instname_str Instituto de Salud Carlos III (ISCIII)
reponame_str Repisalud
collection Repisalud
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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