Effect of deletion and overexpression of tryptophan metabolism genes on growth and fermentation capacity at low temperature in wine yeast

8 pages, 3 figures, 4 tables.-- First published online: 30 APR 2014

Detalles Bibliográficos
Autores: López Malo, María, García Ríos, Estéfani, Martí Raga, María, Chiva, Rosa Ana, Guillamón, José Manuel
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2014
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/113486
Acceso en línea:http://hdl.handle.net/10261/113486
Access Level:acceso abierto
Palabra clave:Cold
Wine
Yeast
Nitrogen
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spelling Effect of deletion and overexpression of tryptophan metabolism genes on growth and fermentation capacity at low temperature in wine yeastLópez Malo, MaríaGarcía Ríos, EstéfaniMartí Raga, MaríaChiva, Rosa AnaGuillamón, José ManuelColdWineYeastNitrogen8 pages, 3 figures, 4 tables.-- First published online: 30 APR 2014Low-temperature fermentations produce wines with greater aromatic complexity, but the success of these fermentations greatly depends on the adaptation of yeast cells to cold. Tryptophan has been previously reported to be a limiting amino acid during Saccharomyces cerevisiae growth at low temperature. The objective of this study was to determine the influence of the tryptophan metabolism on growth and fermentation performance during low-temperature wine fermentation. To this end, we constructed the deletion mutants of the TRP1 and TAT2 genes in a derivative haploid of a commercial wine strain, and the TAT2 gene was overexpressed in the prototroph and auxotroph (Δtrp1) backgrounds. Then we characterized growth and fermentation activity during wine fermentation at low and optimum temperatures. Our results partially support the role of this amino acid in cold yeast growth. Although deletion of TRP1 impaired amino acid uptake and the growth rate at low temperature in synthetic must, this growth impairment did not affect the fermentation rate. Deletion of TAT2 endorsed this strain with the highest nitrogen consumption capacity and the greatest fermentation activity at low temperature.This work has been financially supported by Grants AGL2010-22001-C02-01 from the Spanish government and PROMETEO/2009/019 from the Generalitat Valenciana, awarded to JMG. MLM also thanks the Spanish government for her FPI grant.Peer reviewedAmerican Institute of Chemical EngineersConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201520152014info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/113486reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1002/btpr.1915Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1134862026-05-22T06:33:51Z
dc.title.none.fl_str_mv Effect of deletion and overexpression of tryptophan metabolism genes on growth and fermentation capacity at low temperature in wine yeast
title Effect of deletion and overexpression of tryptophan metabolism genes on growth and fermentation capacity at low temperature in wine yeast
spellingShingle Effect of deletion and overexpression of tryptophan metabolism genes on growth and fermentation capacity at low temperature in wine yeast
López Malo, María
Cold
Wine
Yeast
Nitrogen
title_short Effect of deletion and overexpression of tryptophan metabolism genes on growth and fermentation capacity at low temperature in wine yeast
title_full Effect of deletion and overexpression of tryptophan metabolism genes on growth and fermentation capacity at low temperature in wine yeast
title_fullStr Effect of deletion and overexpression of tryptophan metabolism genes on growth and fermentation capacity at low temperature in wine yeast
title_full_unstemmed Effect of deletion and overexpression of tryptophan metabolism genes on growth and fermentation capacity at low temperature in wine yeast
title_sort Effect of deletion and overexpression of tryptophan metabolism genes on growth and fermentation capacity at low temperature in wine yeast
dc.creator.none.fl_str_mv López Malo, María
García Ríos, Estéfani
Martí Raga, María
Chiva, Rosa Ana
Guillamón, José Manuel
author López Malo, María
author_facet López Malo, María
García Ríos, Estéfani
Martí Raga, María
Chiva, Rosa Ana
Guillamón, José Manuel
author_role author
author2 García Ríos, Estéfani
Martí Raga, María
Chiva, Rosa Ana
Guillamón, José Manuel
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Cold
Wine
Yeast
Nitrogen
topic Cold
Wine
Yeast
Nitrogen
description 8 pages, 3 figures, 4 tables.-- First published online: 30 APR 2014
publishDate 2014
dc.date.none.fl_str_mv 2014
2015
2015
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/113486
url http://hdl.handle.net/10261/113486
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1002/btpr.1915
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Institute of Chemical Engineers
publisher.none.fl_str_mv American Institute of Chemical Engineers
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812455

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