PDL1 signals through conserved sequence motifs to overcome interferon-mediated cytotoxicity

PDL1 blockade produces remarkable clinical responses, thought to occur by T cell reactivation through prevention of PDL1-PD1 T cell inhibitory interactions. Here, we find that PDL1 cell-intrinsic signaling protects cancer cells from interferon (IFN) cytotoxicity and accelerates tumor progression. PD...

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Detalles Bibliográficos
Autores: Gato Cañas, María, Zuazo Ibarra, Miren, Arasanz Esteban, Hugo, Ibañez Vea, María, Lorenzo, Laura, Fernández Hinojal, Gonzalo, Vera García, Ruth, Smerdou, Cristian, Martisova, Eva, Arozarena Martinicorena, Imanol, Wellbrock, Claudia, Llopiz, Diana, Ruiz, Marta, Sarobe, Pablo, Breckpot, Karine, Kochan, Grazyna, Escors Murugarren, David
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2017
País:España
Institución:Universidad Pública de Navarra
Repositorio:Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
OAI Identifier:oai:academica-e.unavarra.es:2454/43728
Acceso en línea:https://hdl.handle.net/2454/43728
Access Level:acceso abierto
Palabra clave:PDL1
PD-L1
B7-H1
CD274
PD1
Interferon
Signal transduction
Immunotherapy
Descripción
Sumario:PDL1 blockade produces remarkable clinical responses, thought to occur by T cell reactivation through prevention of PDL1-PD1 T cell inhibitory interactions. Here, we find that PDL1 cell-intrinsic signaling protects cancer cells from interferon (IFN) cytotoxicity and accelerates tumor progression. PDL1 inhibited IFN signal transduction through a conserved class of sequence motifs that mediate crosstalk with IFN signaling. Abrogation of PDL1 expression or antibody-mediated PDL1 blockade strongly sensitized cancer cells to IFN cytotoxicity through a STAT3/caspase-7-dependent pathway. Moreover, somatic mutations found in human carcinomas within these PDL1 sequence motifs disrupted motif regulation, resulting in PDL1 molecules with enhanced protective activities from type I and type II IFN cytotoxicity. Overall, our results reveal a mode of action of PDL1 in cancer cells as a first line of defense against IFN cytotoxicity.