Cytokine profile and phagocytic capacity of Ndufs4−/− macrophages [Dataset]
(A, B) Parental (Par), and Ndufs4−/− RAW 264.7 cells were left untreated or treated with LPS (100 ng/ml). Supernatants were collected at 8 hours for measurement of cytokine concentrations by ELISA (A). Cells were collected at 4 hours for quantification of cytokine transcripts using real-time PCR (ex...
| Autores: | , , , , , , , , |
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| Formato: | conjunto de datos |
| Estado: | Versión publicada |
| Fecha de publicación: | 2023 |
| País: | España |
| Recursos: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/352276 |
| Acesso em linha: | http://hdl.handle.net/10261/352276 |
| Access Level: | acceso abierto |
| Palavra-chave: | Leigh syndrome patients Inflammatory cytokine profile Increasing evidence demonstrate Div >< p Mitochondrial respiration alterations Macrophage effector functions Effector functions Maximal respiration Vitro </ Supernumerary subunit Shift towards Phagocytose gram Ndufs4 </ Mutational hotspot Modulatory role Lower levels Lipopolysaccharide challenge Improved ability Critical role Atp production |
| Resumo: | (A, B) Parental (Par), and Ndufs4−/− RAW 264.7 cells were left untreated or treated with LPS (100 ng/ml). Supernatants were collected at 8 hours for measurement of cytokine concentrations by ELISA (A). Cells were collected at 4 hours for quantification of cytokine transcripts using real-time PCR (expressed as fold increases versus untreated parental cells) (B). (C) Representative flow cytometry plots (left) showing phagocytosis of FITC labeled heat killed E. coli (HKEC) and bar graph representing % of fluorescent cells and the MFI values (right). Ctrl, control. Ns, not significant; *, P <0.05; **, P <0.01; ***, P<0.005; ****, P<0.001. Each point represents a biological replicate. In all experiments, 4 to 5 different Ndufs4−/− clones were used. Data are shown as the mean ± SEM. |
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