Combining antibacterial and wound healing features: Xanthan gum/guar gum 3D-printed scaffold tuned with hydroxypropyl-β-cyclodextrin/thymol and Zn2+

Biofilm formation on biological and material surfaces represents a heavy health and economic burden for both patient and society. To contrast this phenomenon, medical devices combining antibacterial and pro-wound healing abilities are a promising strategy. In the present work, Xanthan gum/Guar gum (...

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Autores: Virzì, Nicola Filippo, Díaz Rodríguez, Patricia, Concheiro Nine, Ángel Joaquín, Otero Casal, Ana María, Mazzaglia, Antonino, Pittalà, Valeria, Álvarez Lorenzo, Carmen
Tipo de recurso: artículo
Fecha de publicación:2025
País:España
Institución:Universidad de Santiago de Compostela (USC)
Repositorio:Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
Idioma:inglés
OAI Identifier:oai:minerva.usc.gal:10347/42360
Acceso en línea:https://hdl.handle.net/10347/42360
Access Level:acceso abierto
Palabra clave:3D printing
Anti-biofilm
Thymol
Zinc
Isothermal microcalorimetry
Xanthan gum
Guar gum
Cyclodextrin
3209 Farmacología
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spelling Combining antibacterial and wound healing features: Xanthan gum/guar gum 3D-printed scaffold tuned with hydroxypropyl-β-cyclodextrin/thymol and Zn2+Virzì, Nicola FilippoDíaz Rodríguez, PatriciaConcheiro Nine, Ángel JoaquínOtero Casal, Ana MaríaMazzaglia, AntoninoPittalà, ValeriaÁlvarez Lorenzo, Carmen3D printingAnti-biofilmThymolZincIsothermal microcalorimetryXanthan gumGuar gumCyclodextrin3209 FarmacologíaBiofilm formation on biological and material surfaces represents a heavy health and economic burden for both patient and society. To contrast this phenomenon, medical devices combining antibacterial and pro-wound healing abilities are a promising strategy. In the present work, Xanthan gum/Guar gum (XG/GG)-based scaffolds were tuned with thymol and Zn2+ to obtain wound dressings that combine antibacterial and antibiofilm properties and favour the healing process. The tuning process preserved the 3D extrusion-based printability of the XG/GG ink. Scaffolds swelling profile was assessed in PBS pH 7.4, and the resistance to compressive forces was studied using a texturometer. The scaffolds microarchitectures were analyzed by SEM, while ATR-FTIR spotlighted the chemical modifications of the customized materials. Thymol and Zn2+ release was analyzed in biologically relevant media, showing a burst release in the first hours. The antibacterial properties were confirmed against S. aureus, P. aeruginosa, and S. epidermidis by isothermal microcalorimetry and biofilm viable cell counting. Incorporation of hydroxypropyl-β-cyclodextrin (HPβCD) improved thymol loading (7- and 14- times higher thymol content) and enhanced the antimicrobial and antioxidant performances of the dressing, while the presence of Zn2+ strongly potentiated the antimicrobial activity, showing a potent antibiofilm response in both Gram-positive and Gram-negative strains of clinical concern. The thymol and Zn2+ combination led to a reduction of 99.95 %, 99.99 %, and 98.26 %, of biofilm formation against S. aureus, P. aeruginosa, and S. epidermidis, respectively. Furthermore, the scaffolds demonstrated good hemocompatibility, cytocompatibility, tissue integration and pro-angiogenic features in an in ovo CAM model.ElsevierUniversidade de Santiago de Compostela. Departamento de Farmacoloxía, Farmacia e Tecnoloxía FarmacéuticaUniversidade de Santiago de Compostela. Departamento de Microbioloxía e Parasitoloxía20252025-03-0120252025-03-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10347/42360reponame:Minerva. Repositorio Institucional de la Universidad de Santiago de Compostelainstname:Universidad de Santiago de Compostela (USC)InglésengAgencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023 PID2023-150422OB-I00 SCAFFOLDS PIEZOELECTRICOS RECUBIERTOS CON MEMBRANAS CELULARES PARA MEDICINA REGENERATIVA PERSONALIZADAopen accesshttp://purl.org/coar/access_right/c_abf2© 2024 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license. Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:minerva.usc.gal:10347/423602026-06-15T12:47:27Z
dc.title.none.fl_str_mv Combining antibacterial and wound healing features: Xanthan gum/guar gum 3D-printed scaffold tuned with hydroxypropyl-β-cyclodextrin/thymol and Zn2+
title Combining antibacterial and wound healing features: Xanthan gum/guar gum 3D-printed scaffold tuned with hydroxypropyl-β-cyclodextrin/thymol and Zn2+
spellingShingle Combining antibacterial and wound healing features: Xanthan gum/guar gum 3D-printed scaffold tuned with hydroxypropyl-β-cyclodextrin/thymol and Zn2+
Virzì, Nicola Filippo
3D printing
Anti-biofilm
Thymol
Zinc
Isothermal microcalorimetry
Xanthan gum
Guar gum
Cyclodextrin
3209 Farmacología
title_short Combining antibacterial and wound healing features: Xanthan gum/guar gum 3D-printed scaffold tuned with hydroxypropyl-β-cyclodextrin/thymol and Zn2+
title_full Combining antibacterial and wound healing features: Xanthan gum/guar gum 3D-printed scaffold tuned with hydroxypropyl-β-cyclodextrin/thymol and Zn2+
title_fullStr Combining antibacterial and wound healing features: Xanthan gum/guar gum 3D-printed scaffold tuned with hydroxypropyl-β-cyclodextrin/thymol and Zn2+
title_full_unstemmed Combining antibacterial and wound healing features: Xanthan gum/guar gum 3D-printed scaffold tuned with hydroxypropyl-β-cyclodextrin/thymol and Zn2+
title_sort Combining antibacterial and wound healing features: Xanthan gum/guar gum 3D-printed scaffold tuned with hydroxypropyl-β-cyclodextrin/thymol and Zn2+
dc.creator.none.fl_str_mv Virzì, Nicola Filippo
Díaz Rodríguez, Patricia
Concheiro Nine, Ángel Joaquín
Otero Casal, Ana María
Mazzaglia, Antonino
Pittalà, Valeria
Álvarez Lorenzo, Carmen
author Virzì, Nicola Filippo
author_facet Virzì, Nicola Filippo
Díaz Rodríguez, Patricia
Concheiro Nine, Ángel Joaquín
Otero Casal, Ana María
Mazzaglia, Antonino
Pittalà, Valeria
Álvarez Lorenzo, Carmen
author_role author
author2 Díaz Rodríguez, Patricia
Concheiro Nine, Ángel Joaquín
Otero Casal, Ana María
Mazzaglia, Antonino
Pittalà, Valeria
Álvarez Lorenzo, Carmen
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade de Santiago de Compostela. Departamento de Farmacoloxía, Farmacia e Tecnoloxía Farmacéutica
Universidade de Santiago de Compostela. Departamento de Microbioloxía e Parasitoloxía
dc.subject.none.fl_str_mv 3D printing
Anti-biofilm
Thymol
Zinc
Isothermal microcalorimetry
Xanthan gum
Guar gum
Cyclodextrin
3209 Farmacología
topic 3D printing
Anti-biofilm
Thymol
Zinc
Isothermal microcalorimetry
Xanthan gum
Guar gum
Cyclodextrin
3209 Farmacología
description Biofilm formation on biological and material surfaces represents a heavy health and economic burden for both patient and society. To contrast this phenomenon, medical devices combining antibacterial and pro-wound healing abilities are a promising strategy. In the present work, Xanthan gum/Guar gum (XG/GG)-based scaffolds were tuned with thymol and Zn2+ to obtain wound dressings that combine antibacterial and antibiofilm properties and favour the healing process. The tuning process preserved the 3D extrusion-based printability of the XG/GG ink. Scaffolds swelling profile was assessed in PBS pH 7.4, and the resistance to compressive forces was studied using a texturometer. The scaffolds microarchitectures were analyzed by SEM, while ATR-FTIR spotlighted the chemical modifications of the customized materials. Thymol and Zn2+ release was analyzed in biologically relevant media, showing a burst release in the first hours. The antibacterial properties were confirmed against S. aureus, P. aeruginosa, and S. epidermidis by isothermal microcalorimetry and biofilm viable cell counting. Incorporation of hydroxypropyl-β-cyclodextrin (HPβCD) improved thymol loading (7- and 14- times higher thymol content) and enhanced the antimicrobial and antioxidant performances of the dressing, while the presence of Zn2+ strongly potentiated the antimicrobial activity, showing a potent antibiofilm response in both Gram-positive and Gram-negative strains of clinical concern. The thymol and Zn2+ combination led to a reduction of 99.95 %, 99.99 %, and 98.26 %, of biofilm formation against S. aureus, P. aeruginosa, and S. epidermidis, respectively. Furthermore, the scaffolds demonstrated good hemocompatibility, cytocompatibility, tissue integration and pro-angiogenic features in an in ovo CAM model.
publishDate 2025
dc.date.none.fl_str_mv 2025
2025-03-01
2025
2025-03-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/10347/42360
url https://hdl.handle.net/10347/42360
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023 PID2023-150422OB-I00 SCAFFOLDS PIEZOELECTRICOS RECUBIERTOS CON MEMBRANAS CELULARES PARA MEDICINA REGENERATIVA PERSONALIZADA
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
instname:Universidad de Santiago de Compostela (USC)
instname_str Universidad de Santiago de Compostela (USC)
reponame_str Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
collection Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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