Classical Swine Fever Virus vs. Classical Swine Fever Virus

Two groups with three wild boars each were used: Group A (animals 1 to 3) served as the control, and Group B (animals 4 to 6) was postnatally persistently infected with the Cat01 strain of CSFV (primary virus). The animals, six weeks old and clinically healthy, were inoculated with the virulent stra...

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Autores: Muñoz González, Sara, Pérez-Simó, Marta, Colom Cadena, Andreu|||0000-0003-0983-2412, Cabezón Ponsoda, Óscar|||0000-0001-7543-8371, Bohórquez Garzón, José Alejandro|||0000-0002-6715-1757, Rosell, Rosa|||0000-0002-8294-1159, Pérez, Lester Josue|||0000-0002-5717-5181, Marco, Ignasi|||0000-0001-7479-3419, Lavín González, Santiago|||0000-0001-5655-588X, Domingo, Mariano|||0000-0002-9623-4826, Ganges, Llilianne|||0000-0002-8644-3560
Tipo de recurso: artículo
Fecha de publicación:2016
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:170615
Acceso en línea:https://ddd.uab.cat/record/170615
https://dx.doi.org/urn:doi:10.1371/journal.pone.0149469
Access Level:acceso abierto
Palabra clave:RNA extraction
Swine
Superinfection
Enzyme-linked immunoassays
Sequence analysis
Viral persistence and latency
Immune response
RNA viruses
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spelling Classical Swine Fever Virus vs. Classical Swine Fever Virusthe Superinfection Exclusion Phenomenon in Experimentally Infected Wild BoarMuñoz González, SaraPérez-Simó, MartaColom Cadena, Andreu|||0000-0003-0983-2412Cabezón Ponsoda, Óscar|||0000-0001-7543-8371Bohórquez Garzón, José Alejandro|||0000-0002-6715-1757Rosell, Rosa|||0000-0002-8294-1159Pérez, Lester Josue|||0000-0002-5717-5181Marco, Ignasi|||0000-0001-7479-3419Lavín González, Santiago|||0000-0001-5655-588XDomingo, Mariano|||0000-0002-9623-4826Ganges, Llilianne|||0000-0002-8644-3560RNA extractionSwineSuperinfectionEnzyme-linked immunoassaysSequence analysisViral persistence and latencyImmune responseRNA virusesTwo groups with three wild boars each were used: Group A (animals 1 to 3) served as the control, and Group B (animals 4 to 6) was postnatally persistently infected with the Cat01 strain of CSFV (primary virus). The animals, six weeks old and clinically healthy, were inoculated with the virulent strain Margarita (secondary virus). For exclusive detection of the Margarita strain, a specific qRT-PCR assay was designed, which proved not to have cross-reactivity with the Cat01 strain. The wild boars persistently infected with CSFV were protected from superinfection by the virulent CSFV Margarita strain, as evidenced by the absence of clinical signs and the absence of Margarita RNA detection in serum, swabs and tissue samples. Additionally, in PBMCs, a well-known target for CSFV viral replication, only the primary infecting virus RNA (Cat01 strain) could be detected, even after the isolation in ST cells, demonstrating SIE at the tissue level in vivo. Furthermore, the data analysis of the Margarita qRT-PCR, by means of calculated ΔCt values, supported that PBMCs from persistently infected animals were substantially protected from superinfection after in vitro inoculation with the Margarita virus strain, while this virus was able to infect naive PBMCs efficiently. In parallel, IFN-α values were undetectable in the sera from animals in Group B after inoculation with the CSFV Margarita strain. Furthermore, these animals were unable to elicit adaptive humoral (no E2-specific or neutralising antibodies) or cellular immune responses (in terms of IFN-γ-producing cells) after inoculation with the second virus. Finally, a sequence analysis could not detect CSFV Margarita RNA in the samples tested from Group B. Our results suggested that the SIE phenomenon might be involved in the evolution and phylogeny of the virus, as well as in CSFV control by vaccination. To the best of our knowledge, this study was one of the first showing efficient suppression of superinfection in animals, especially in the absence of IFN-α, which might be associated with the lack of innate immune mechanisms. 22016-01-0120162016-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/170615https://dx.doi.org/urn:doi:10.1371/journal.pone.0149469reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengopen accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:1706152026-06-06T12:50:31Z
dc.title.none.fl_str_mv Classical Swine Fever Virus vs. Classical Swine Fever Virus
the Superinfection Exclusion Phenomenon in Experimentally Infected Wild Boar
title Classical Swine Fever Virus vs. Classical Swine Fever Virus
spellingShingle Classical Swine Fever Virus vs. Classical Swine Fever Virus
Muñoz González, Sara
RNA extraction
Swine
Superinfection
Enzyme-linked immunoassays
Sequence analysis
Viral persistence and latency
Immune response
RNA viruses
title_short Classical Swine Fever Virus vs. Classical Swine Fever Virus
title_full Classical Swine Fever Virus vs. Classical Swine Fever Virus
title_fullStr Classical Swine Fever Virus vs. Classical Swine Fever Virus
title_full_unstemmed Classical Swine Fever Virus vs. Classical Swine Fever Virus
title_sort Classical Swine Fever Virus vs. Classical Swine Fever Virus
dc.creator.none.fl_str_mv Muñoz González, Sara
Pérez-Simó, Marta
Colom Cadena, Andreu|||0000-0003-0983-2412
Cabezón Ponsoda, Óscar|||0000-0001-7543-8371
Bohórquez Garzón, José Alejandro|||0000-0002-6715-1757
Rosell, Rosa|||0000-0002-8294-1159
Pérez, Lester Josue|||0000-0002-5717-5181
Marco, Ignasi|||0000-0001-7479-3419
Lavín González, Santiago|||0000-0001-5655-588X
Domingo, Mariano|||0000-0002-9623-4826
Ganges, Llilianne|||0000-0002-8644-3560
author Muñoz González, Sara
author_facet Muñoz González, Sara
Pérez-Simó, Marta
Colom Cadena, Andreu|||0000-0003-0983-2412
Cabezón Ponsoda, Óscar|||0000-0001-7543-8371
Bohórquez Garzón, José Alejandro|||0000-0002-6715-1757
Rosell, Rosa|||0000-0002-8294-1159
Pérez, Lester Josue|||0000-0002-5717-5181
Marco, Ignasi|||0000-0001-7479-3419
Lavín González, Santiago|||0000-0001-5655-588X
Domingo, Mariano|||0000-0002-9623-4826
Ganges, Llilianne|||0000-0002-8644-3560
author_role author
author2 Pérez-Simó, Marta
Colom Cadena, Andreu|||0000-0003-0983-2412
Cabezón Ponsoda, Óscar|||0000-0001-7543-8371
Bohórquez Garzón, José Alejandro|||0000-0002-6715-1757
Rosell, Rosa|||0000-0002-8294-1159
Pérez, Lester Josue|||0000-0002-5717-5181
Marco, Ignasi|||0000-0001-7479-3419
Lavín González, Santiago|||0000-0001-5655-588X
Domingo, Mariano|||0000-0002-9623-4826
Ganges, Llilianne|||0000-0002-8644-3560
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv RNA extraction
Swine
Superinfection
Enzyme-linked immunoassays
Sequence analysis
Viral persistence and latency
Immune response
RNA viruses
topic RNA extraction
Swine
Superinfection
Enzyme-linked immunoassays
Sequence analysis
Viral persistence and latency
Immune response
RNA viruses
description Two groups with three wild boars each were used: Group A (animals 1 to 3) served as the control, and Group B (animals 4 to 6) was postnatally persistently infected with the Cat01 strain of CSFV (primary virus). The animals, six weeks old and clinically healthy, were inoculated with the virulent strain Margarita (secondary virus). For exclusive detection of the Margarita strain, a specific qRT-PCR assay was designed, which proved not to have cross-reactivity with the Cat01 strain. The wild boars persistently infected with CSFV were protected from superinfection by the virulent CSFV Margarita strain, as evidenced by the absence of clinical signs and the absence of Margarita RNA detection in serum, swabs and tissue samples. Additionally, in PBMCs, a well-known target for CSFV viral replication, only the primary infecting virus RNA (Cat01 strain) could be detected, even after the isolation in ST cells, demonstrating SIE at the tissue level in vivo. Furthermore, the data analysis of the Margarita qRT-PCR, by means of calculated ΔCt values, supported that PBMCs from persistently infected animals were substantially protected from superinfection after in vitro inoculation with the Margarita virus strain, while this virus was able to infect naive PBMCs efficiently. In parallel, IFN-α values were undetectable in the sera from animals in Group B after inoculation with the CSFV Margarita strain. Furthermore, these animals were unable to elicit adaptive humoral (no E2-specific or neutralising antibodies) or cellular immune responses (in terms of IFN-γ-producing cells) after inoculation with the second virus. Finally, a sequence analysis could not detect CSFV Margarita RNA in the samples tested from Group B. Our results suggested that the SIE phenomenon might be involved in the evolution and phylogeny of the virus, as well as in CSFV control by vaccination. To the best of our knowledge, this study was one of the first showing efficient suppression of superinfection in animals, especially in the absence of IFN-α, which might be associated with the lack of innate immune mechanisms.
publishDate 2016
dc.date.none.fl_str_mv 2
2016-01-01
2016
2016-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/170615
https://dx.doi.org/urn:doi:10.1371/journal.pone.0149469
url https://ddd.uab.cat/record/170615
https://dx.doi.org/urn:doi:10.1371/journal.pone.0149469
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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