Phycomyces MADB interacts with MADA to form the primary photoreceptor complex for fungal phototropism.

[EN] The fungus Phycomyces blakesleeanus reacts to environmental signals, including light, gravity, touch, and the presence of nearby objects, by changing the speed and direction of growth of its fruiting body (sporangiophore). Phototropism, growth toward light, shares many features in fungi and pla...

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Autores: Sanz Lozano, Catalina Sofía, Rodríguez-Romero, Julio, Idnurm, Alexander, Christie, John M, Heitman, Joseph, Corrochano, Luis M, Pérez Eslava, Arturo
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2009
País:España
Institución:Universidad de Salamanca (USAL)
Repositorio:GREDOS. Repositorio Institucional de la Universidad de Salamanca
OAI Identifier:oai:gredos.usal.es:10366/158987
Acceso en línea:http://hdl.handle.net/10366/158987
Access Level:acceso abierto
Palabra clave:Phycomyces blakesleeanus
phototropism
photoreceptor
MADA
MADB
Genetics
Base Sequence
DNA-Binding Proteins
Color
Fungal Proteins
Photoreceptor Cells
Mutation
Phycomyces
Phylogeny
Phototropism
Genome
Sequence Alignment
Alternative Splicing
Transcription Factors
2409 Genética
mutación
secuencia de bases
genoma
alineación de secuencias
color
proteínas fúngicas
fototropismo
proteínas de unión al ADN
empalme alternativo
filogenia
factores de transcripción
genética
células fotorreceptoras
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network_acronym_str ES
network_name_str España
repository_id_str
spelling Phycomyces MADB interacts with MADA to form the primary photoreceptor complex for fungal phototropism.Sanz Lozano, Catalina SofíaRodríguez-Romero, JulioIdnurm, AlexanderChristie, John MHeitman, JosephCorrochano, Luis MPérez Eslava, ArturoPhycomyces blakesleeanusphototropismphotoreceptorMADAMADBGeneticsBase SequenceDNA-Binding ProteinsColorFungal ProteinsPhotoreceptor CellsMutationPhycomycesPhylogenyPhototropismGenomeSequence AlignmentAlternative SplicingTranscription Factors2409 Genéticamutaciónsecuencia de basesgenomaalineación de secuenciascolorproteínas fúngicasfototropismoproteínas de unión al ADNPhycomycesempalme alternativofilogeniafactores de transcripcióngenéticacélulas fotorreceptoras[EN] The fungus Phycomyces blakesleeanus reacts to environmental signals, including light, gravity, touch, and the presence of nearby objects, by changing the speed and direction of growth of its fruiting body (sporangiophore). Phototropism, growth toward light, shares many features in fungi and plants but the molecular mechanisms remain to be fully elucidated. Phycomyces mutants with altered phototropism were isolated approximately 40 years ago and found to have mutations in the mad genes. All of the responses to light in Phycomyces require the products of the madA and madB genes. We showed that madA encodes a protein similar to the Neurospora blue-light photoreceptor, zinc-finger protein WC-1. We show here that madB encodes a protein similar to the Neurospora zinc-finger protein WC-2. MADA and MADB interact to form a complex in yeast 2-hybrid assays and when coexpressed in E. coli, providing evidence that phototropism and other responses to light are mediated by a photoresponsive transcription factor complex. The Phycomyces genome contains 3 genes similar to wc-1, and 4 genes similar to wc-2, many of which are regulated by light in a madA or madB dependent manner. We did not detect any interactions between additional WC proteins in yeast 2-hybrid assays, which suggest that MADA and MADB form the major photoreceptor complex in Phycomyces. However, the presence of multiple wc genes in Phycomyces may enable perception across a broad range of light intensities, and may provide specialized photoreceptors for distinct photoresponses.European funds (ERDF); Junta de Castilla y León; Junta de AndalucíaPNAS202420242009info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10366/158987reponame:GREDOS. Repositorio Institucional de la Universidad de Salamancainstname:Universidad de Salamanca (USAL)InglésAttribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:gredos.usal.es:10366/1589872026-06-07T06:28:51Z
dc.title.none.fl_str_mv Phycomyces MADB interacts with MADA to form the primary photoreceptor complex for fungal phototropism.
title Phycomyces MADB interacts with MADA to form the primary photoreceptor complex for fungal phototropism.
spellingShingle Phycomyces MADB interacts with MADA to form the primary photoreceptor complex for fungal phototropism.
Sanz Lozano, Catalina Sofía
Phycomyces blakesleeanus
phototropism
photoreceptor
MADA
MADB
Genetics
Base Sequence
DNA-Binding Proteins
Color
Fungal Proteins
Photoreceptor Cells
Mutation
Phycomyces
Phylogeny
Phototropism
Genome
Sequence Alignment
Alternative Splicing
Transcription Factors
2409 Genética
mutación
secuencia de bases
genoma
alineación de secuencias
color
proteínas fúngicas
fototropismo
proteínas de unión al ADN
Phycomyces
empalme alternativo
filogenia
factores de transcripción
genética
células fotorreceptoras
title_short Phycomyces MADB interacts with MADA to form the primary photoreceptor complex for fungal phototropism.
title_full Phycomyces MADB interacts with MADA to form the primary photoreceptor complex for fungal phototropism.
title_fullStr Phycomyces MADB interacts with MADA to form the primary photoreceptor complex for fungal phototropism.
title_full_unstemmed Phycomyces MADB interacts with MADA to form the primary photoreceptor complex for fungal phototropism.
title_sort Phycomyces MADB interacts with MADA to form the primary photoreceptor complex for fungal phototropism.
dc.creator.none.fl_str_mv Sanz Lozano, Catalina Sofía
Rodríguez-Romero, Julio
Idnurm, Alexander
Christie, John M
Heitman, Joseph
Corrochano, Luis M
Pérez Eslava, Arturo
author Sanz Lozano, Catalina Sofía
author_facet Sanz Lozano, Catalina Sofía
Rodríguez-Romero, Julio
Idnurm, Alexander
Christie, John M
Heitman, Joseph
Corrochano, Luis M
Pérez Eslava, Arturo
author_role author
author2 Rodríguez-Romero, Julio
Idnurm, Alexander
Christie, John M
Heitman, Joseph
Corrochano, Luis M
Pérez Eslava, Arturo
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Phycomyces blakesleeanus
phototropism
photoreceptor
MADA
MADB
Genetics
Base Sequence
DNA-Binding Proteins
Color
Fungal Proteins
Photoreceptor Cells
Mutation
Phycomyces
Phylogeny
Phototropism
Genome
Sequence Alignment
Alternative Splicing
Transcription Factors
2409 Genética
mutación
secuencia de bases
genoma
alineación de secuencias
color
proteínas fúngicas
fototropismo
proteínas de unión al ADN
Phycomyces
empalme alternativo
filogenia
factores de transcripción
genética
células fotorreceptoras
topic Phycomyces blakesleeanus
phototropism
photoreceptor
MADA
MADB
Genetics
Base Sequence
DNA-Binding Proteins
Color
Fungal Proteins
Photoreceptor Cells
Mutation
Phycomyces
Phylogeny
Phototropism
Genome
Sequence Alignment
Alternative Splicing
Transcription Factors
2409 Genética
mutación
secuencia de bases
genoma
alineación de secuencias
color
proteínas fúngicas
fototropismo
proteínas de unión al ADN
Phycomyces
empalme alternativo
filogenia
factores de transcripción
genética
células fotorreceptoras
description [EN] The fungus Phycomyces blakesleeanus reacts to environmental signals, including light, gravity, touch, and the presence of nearby objects, by changing the speed and direction of growth of its fruiting body (sporangiophore). Phototropism, growth toward light, shares many features in fungi and plants but the molecular mechanisms remain to be fully elucidated. Phycomyces mutants with altered phototropism were isolated approximately 40 years ago and found to have mutations in the mad genes. All of the responses to light in Phycomyces require the products of the madA and madB genes. We showed that madA encodes a protein similar to the Neurospora blue-light photoreceptor, zinc-finger protein WC-1. We show here that madB encodes a protein similar to the Neurospora zinc-finger protein WC-2. MADA and MADB interact to form a complex in yeast 2-hybrid assays and when coexpressed in E. coli, providing evidence that phototropism and other responses to light are mediated by a photoresponsive transcription factor complex. The Phycomyces genome contains 3 genes similar to wc-1, and 4 genes similar to wc-2, many of which are regulated by light in a madA or madB dependent manner. We did not detect any interactions between additional WC proteins in yeast 2-hybrid assays, which suggest that MADA and MADB form the major photoreceptor complex in Phycomyces. However, the presence of multiple wc genes in Phycomyces may enable perception across a broad range of light intensities, and may provide specialized photoreceptors for distinct photoresponses.
publishDate 2009
dc.date.none.fl_str_mv 2009
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10366/158987
url http://hdl.handle.net/10366/158987
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv PNAS
publisher.none.fl_str_mv PNAS
dc.source.none.fl_str_mv reponame:GREDOS. Repositorio Institucional de la Universidad de Salamanca
instname:Universidad de Salamanca (USAL)
instname_str Universidad de Salamanca (USAL)
reponame_str GREDOS. Repositorio Institucional de la Universidad de Salamanca
collection GREDOS. Repositorio Institucional de la Universidad de Salamanca
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869404662647488512
score 15,811543

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