A Ras GTPase associated protein is involved in the phototropic and circadian photobiology responses in fungi

[EN] Light is an environmental signal perceived by most eukaryotic organisms and that can have major impacts on their growth and development. The MadC protein in the fungus Phycomyces blakesleeanus (Mucoromycotina) has been postulated to form part of the photosensory input for phototropism of the fr...

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Detalhes bibliográficos
Autores: Polaino, Silvia, Villalobos-Escobedo, José M., Shakya, Viplendra P. S., Miralles-Durán, Alejandro, Chaudhary, Suman, Sanz Lozano, Catalina Sofía, Shahriari, Mahdi, Luque, Eva M., Eslava, Arturo P., Corrochano, Luis M., Herrera-Estrella, Alfredo, Idnurm, Alexander
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2017
País:España
Recursos:Universidad de Salamanca (USAL)
Repositorio:GREDOS. Repositorio Institucional de la Universidad de Salamanca
OAI Identifier:oai:gredos.usal.es:10366/158862
Acesso em linha:http://hdl.handle.net/10366/158862
Access Level:acceso abierto
Palavra-chave:Fungus
Phycomyces blakesleeanus
MadC gene
Phototropism
Genetics
2409 Genética
genética
Descrição
Resumo:[EN] Light is an environmental signal perceived by most eukaryotic organisms and that can have major impacts on their growth and development. The MadC protein in the fungus Phycomyces blakesleeanus (Mucoromycotina) has been postulated to form part of the photosensory input for phototropism of the fruiting body sporangiophores, but the madC gene has remained unidentified since the 1960s when madC mutants were first isolated. In this study the madC gene was identified by positional cloning. All madC mutant strains contain loss-of-function point mutations within a gene predicted to encode a GTPase activating protein (GAP) for Ras. The madC gene complements the Saccharomyces cerevisiae Ras-GAP ira1 mutant and the encoded MadC protein interacts with P. blakesleeanus Ras homologs in yeast two-hybrid assays, indicating that MadC is a regulator of Ras signaling. Deletion of the homolog in the filamentous ascomycete Neurospora crassa affects the circadian clock output, yielding a pattern of asexual conidiation similar to a ras-1 mutant that is used in circadian studies in N. crassa. Thus, MadC is unlikely to be a photosensor, yet is a fundamental link in the photoresponses from blue light perceived by the conserved White Collar complex with Ras signaling in two distantly-related filamentous fungal species.

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