Understanding the molecular mechanism of the Ala-versus-Gly concept controlling the pProduct specificity in reactions catalyzed by lipoxygenases
Lipoxygenases (LOXs) are a family of enzymes that catalyze the highly specific hydroperoxidation of polyunsaturated fatty acids, such as arachidonic acid. Different stereo- or/and regioisomer hydroperoxidation products lead later to different metabolites that exert opposite physiological effects in...
| Authors: | , , , , , |
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| Format: | article |
| Publication Date: | 2017 |
| Country: | España |
| Institution: | Universitat Autònoma de Barcelona |
| Repository: | Dipòsit Digital de Documents de la UAB |
| Language: | English |
| OAI Identifier: | oai:ddd.uab.cat:225211 |
| Online Access: | https://ddd.uab.cat/record/225211 https://dx.doi.org/urn:doi:10.1021/acscatal.7b00842 |
| Access Level: | Open access |
| Keyword: | Coral 8R-lipoxygenase Ala-versus-Gly concept Enzyme catalysis Arachidonic acid QM/MM calculations Molecular dynamics simulations Free energy calculations |
| Summary: | Lipoxygenases (LOXs) are a family of enzymes that catalyze the highly specific hydroperoxidation of polyunsaturated fatty acids, such as arachidonic acid. Different stereo- or/and regioisomer hydroperoxidation products lead later to different metabolites that exert opposite physiological effects in the animal body and play a central role in inflammatory processes. The Gly-Ala switch of a single residue is crucial for the stereo- and regiocontrol in many lipoxygenases. Herein, we have combined molecular dynamics simulations with quantum mechanics/molecular mechanics calculations to study the hydrogen abstraction step and the molecular oxygen addition step of the hydroperoxidation reaction of arachidonic acid catalyzed by both wild-type Coral 8R-LOX and its Gly427Ala mutant. We have obtained a detailed molecular understanding of this Ala-versus-Gly concept. In wild type, molecular oxygen adds to C of arachidonic acid with an R stereochemistry. In the mutant, Ala427 pushes Leu385, blocks the region over C, and opens an oxygen access channel now directed to C, where molecular oxygen is added with an S stereochemistry. Thus, the specificity turns out to be dramatically inverted. Since Leu385 is highly conserved among many lipoxygenase isoforms, this mechanism can be general, and we propose that the presence of such type of bulky and hydrophobic residues can be key in controlling the extreme regio- and stereospecificity of lipoxygenases and, as a consequence, their physiological effects. |
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