Accomplished High-Resolution Metabolomic and Molecular Studies Identify New Carotenoid Biosynthetic Reactions in Cyanobacteria

3 Figuras.-- 3 Tablas

Detalles Bibliográficos
Autores: Manzoni Maroneze, Mariana, Caballero-Guerrero, Belén, Queiroz Zepka, Leila, Jacob-Lopes, Eduardo, Pérez Gálvez, Antonio, Roca, María
Tipo de recurso: artículo
Fecha de publicación:2020
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/215822
Acceso en línea:http://hdl.handle.net/10261/215822
Access Level:acceso abierto
Palabra clave:Carotenoids
α-cryptoxanthin
Cyanobacteria
2′-dehydrodeoxomyxol
Metabolomics
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spelling Accomplished High-Resolution Metabolomic and Molecular Studies Identify New Carotenoid Biosynthetic Reactions in CyanobacteriaManzoni Maroneze, MarianaCaballero-Guerrero, BelénQueiroz Zepka, LeilaJacob-Lopes, EduardoPérez Gálvez, AntonioRoca, MaríaCarotenoidsα-cryptoxanthinCyanobacteria2′-dehydrodeoxomyxolMetabolomics3 Figuras.-- 3 TablasCyanobacteria and microalgae are characterized by a rich and varied profile of chlorophyll (8–20 mg/g) and carotenoid (>2.2 mg/g) pigments, being noteworthy material for natural pigment production in the food industry. We propose a systematic workflow that uses high-performance liquid chromatography (HPLC) coupled with high-resolution tandem mass spectrometry in a broadband collision-induced dissociation mode (bbCID) acquisition mode to simultaneously obtain MS and MSn spectra. Metabolomic studies showed for the first time the presence of carotenoids with 5,6-epoxy-groups (5,6-epoxy- and 5,8-furanoid β-cryptoxanthin), carotenoids from the α-branch (5,8-furanoid-2′-3′-didehydro α-cryptoxanthin), and 2′-dehydrodeoxomyxol in cyanobacteria. To support the new findings, an in silico search retrieved the putative sequences of carotenogenic enzymes involved in the corresponding biosynthetic pathways (ZEP, NSY, CrtL-b and CrtR) in the analyzed cyanobacteria species. Consequently, high-throughput metabolomics studies assisted by molecular analysis offer a powerful tool for providing insights into the characterization of bioactive compounds and their metabolism in cyanobacteria and microalgae.This work was supported by the Ministerio de Ciencia, Investigación y Universidades, Agencia Estatal de Investigación y Fondo Europeo de Desarrollo Regional (FEDER), grant number RTI2018-095415-B-I00. M.M. was supported with a fellowship from CAPES.Peer reviewedAmerican Chemical SocietyMinisterio de Ciencia, Innovación y Universidades (España)Agencia Estatal de Investigación (España)European CommissionConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202020202020info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/215822reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-095415-B-I00http://dx.doi.org/10.1021/acs.jafc.0c01306Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2158222026-05-22T06:33:51Z
dc.title.none.fl_str_mv Accomplished High-Resolution Metabolomic and Molecular Studies Identify New Carotenoid Biosynthetic Reactions in Cyanobacteria
title Accomplished High-Resolution Metabolomic and Molecular Studies Identify New Carotenoid Biosynthetic Reactions in Cyanobacteria
spellingShingle Accomplished High-Resolution Metabolomic and Molecular Studies Identify New Carotenoid Biosynthetic Reactions in Cyanobacteria
Manzoni Maroneze, Mariana
Carotenoids
α-cryptoxanthin
Cyanobacteria
2′-dehydrodeoxomyxol
Metabolomics
title_short Accomplished High-Resolution Metabolomic and Molecular Studies Identify New Carotenoid Biosynthetic Reactions in Cyanobacteria
title_full Accomplished High-Resolution Metabolomic and Molecular Studies Identify New Carotenoid Biosynthetic Reactions in Cyanobacteria
title_fullStr Accomplished High-Resolution Metabolomic and Molecular Studies Identify New Carotenoid Biosynthetic Reactions in Cyanobacteria
title_full_unstemmed Accomplished High-Resolution Metabolomic and Molecular Studies Identify New Carotenoid Biosynthetic Reactions in Cyanobacteria
title_sort Accomplished High-Resolution Metabolomic and Molecular Studies Identify New Carotenoid Biosynthetic Reactions in Cyanobacteria
dc.creator.none.fl_str_mv Manzoni Maroneze, Mariana
Caballero-Guerrero, Belén
Queiroz Zepka, Leila
Jacob-Lopes, Eduardo
Pérez Gálvez, Antonio
Roca, María
author Manzoni Maroneze, Mariana
author_facet Manzoni Maroneze, Mariana
Caballero-Guerrero, Belén
Queiroz Zepka, Leila
Jacob-Lopes, Eduardo
Pérez Gálvez, Antonio
Roca, María
author_role author
author2 Caballero-Guerrero, Belén
Queiroz Zepka, Leila
Jacob-Lopes, Eduardo
Pérez Gálvez, Antonio
Roca, María
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia, Innovación y Universidades (España)
Agencia Estatal de Investigación (España)
European Commission
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Carotenoids
α-cryptoxanthin
Cyanobacteria
2′-dehydrodeoxomyxol
Metabolomics
topic Carotenoids
α-cryptoxanthin
Cyanobacteria
2′-dehydrodeoxomyxol
Metabolomics
description 3 Figuras.-- 3 Tablas
publishDate 2020
dc.date.none.fl_str_mv 2020
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/215822
url http://hdl.handle.net/10261/215822
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-095415-B-I00
http://dx.doi.org/10.1021/acs.jafc.0c01306
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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