Cryotolerance of stallion spermatozoa relies on aquaglyceroporins rather than orthodox aquaporins

Aquaporins (AQPs), a family of ubiquitous water channels divided into orthodox AQPs, aquaglyceroporins (GLPs), and superAQPs, are present in stallion spermatozoa. The aim of this study was to elucidate the functional relevance of each group of AQPs during stallion sperm cryopreservation through the...

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Autores: Delgado Bermudez, Ariadna|||0000-0001-5655-5003, Noto, Federico, Bonilla Correal, Sebastian|||0000-0003-1015-5017, Garcia-Bonavila, Estela, Catalán, Jaime|||0000-0002-0878-7819, Papas, Marion|||0000-0001-8610-2734, Bonet, Sergi|||0000-0002-5125-7274, Miró, Jordi|||0000-0002-1085-0039, Yeste Oliveras, Marc|||0000-0002-2209-340X
Tipo de recurso: artículo
Fecha de publicación:2019
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:216719
Acceso en línea:https://ddd.uab.cat/record/216719
https://dx.doi.org/urn:doi:10.3390/biology8040085
Access Level:acceso abierto
Palabra clave:Acetazolamide
Aquaporins
Phloretin
Propanediol
Sperm
Stallion
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spelling Cryotolerance of stallion spermatozoa relies on aquaglyceroporins rather than orthodox aquaporinsDelgado Bermudez, Ariadna|||0000-0001-5655-5003Noto, FedericoBonilla Correal, Sebastian|||0000-0003-1015-5017Garcia-Bonavila, EstelaCatalán, Jaime|||0000-0002-0878-7819Papas, Marion|||0000-0001-8610-2734Bonet, Sergi|||0000-0002-5125-7274Miró, Jordi|||0000-0002-1085-0039Yeste Oliveras, Marc|||0000-0002-2209-340XAcetazolamideAquaporinsPhloretinPropanediolSpermStallionAquaporins (AQPs), a family of ubiquitous water channels divided into orthodox AQPs, aquaglyceroporins (GLPs), and superAQPs, are present in stallion spermatozoa. The aim of this study was to elucidate the functional relevance of each group of AQPs during stallion sperm cryopreservation through the use of three different inhibitors: acetazolamide (AC), phloretin (PHL) and propanediol (PDO). Sperm quality and function parameters were evaluated in the presence or absence of each inhibitor in fresh and frozen-thawed samples. In the presence of AC, different parameters were altered (p < 0.05), but not in a concentration-or time-depending manner. PHL was found to decrease sperm motility, viability, acrosome integrity, and the percentages of spermatozoa with low membrane lipid disorder, high mitochondrial membrane potential (MMP) and high intracellular levels of calcium and superoxides (p < 0.05). Finally, the sperm motility, viability, acrosome integrity, the percentages of spermatozoa with low membrane lipid disorder, high MMP and high intracellular calcium levels were higher (p < 0.05) in PDO treatments than in the control. The sperm response to AC, PHL and PDO indicates that GLPs, rather than orthodox AQPs, play a crucial role during stallion sperm cryopreservation. Furthermore, post-thaw sperm quality was higher in PDO treatments than in the control, suggesting that this molecule is a potential permeable cryoprotectant. 22019-01-0120192019-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/216719https://dx.doi.org/urn:doi:10.3390/biology8040085reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengAgència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2017/SGR-1229Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 AGL2017-88329-RMinisterio de Economía y Competitividad https://doi.org/10.13039/501100003329 PRE2018-083488Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 RYC-2014-15581open accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2167192026-06-06T12:50:31Z
dc.title.none.fl_str_mv Cryotolerance of stallion spermatozoa relies on aquaglyceroporins rather than orthodox aquaporins
title Cryotolerance of stallion spermatozoa relies on aquaglyceroporins rather than orthodox aquaporins
spellingShingle Cryotolerance of stallion spermatozoa relies on aquaglyceroporins rather than orthodox aquaporins
Delgado Bermudez, Ariadna|||0000-0001-5655-5003
Acetazolamide
Aquaporins
Phloretin
Propanediol
Sperm
Stallion
title_short Cryotolerance of stallion spermatozoa relies on aquaglyceroporins rather than orthodox aquaporins
title_full Cryotolerance of stallion spermatozoa relies on aquaglyceroporins rather than orthodox aquaporins
title_fullStr Cryotolerance of stallion spermatozoa relies on aquaglyceroporins rather than orthodox aquaporins
title_full_unstemmed Cryotolerance of stallion spermatozoa relies on aquaglyceroporins rather than orthodox aquaporins
title_sort Cryotolerance of stallion spermatozoa relies on aquaglyceroporins rather than orthodox aquaporins
dc.creator.none.fl_str_mv Delgado Bermudez, Ariadna|||0000-0001-5655-5003
Noto, Federico
Bonilla Correal, Sebastian|||0000-0003-1015-5017
Garcia-Bonavila, Estela
Catalán, Jaime|||0000-0002-0878-7819
Papas, Marion|||0000-0001-8610-2734
Bonet, Sergi|||0000-0002-5125-7274
Miró, Jordi|||0000-0002-1085-0039
Yeste Oliveras, Marc|||0000-0002-2209-340X
author Delgado Bermudez, Ariadna|||0000-0001-5655-5003
author_facet Delgado Bermudez, Ariadna|||0000-0001-5655-5003
Noto, Federico
Bonilla Correal, Sebastian|||0000-0003-1015-5017
Garcia-Bonavila, Estela
Catalán, Jaime|||0000-0002-0878-7819
Papas, Marion|||0000-0001-8610-2734
Bonet, Sergi|||0000-0002-5125-7274
Miró, Jordi|||0000-0002-1085-0039
Yeste Oliveras, Marc|||0000-0002-2209-340X
author_role author
author2 Noto, Federico
Bonilla Correal, Sebastian|||0000-0003-1015-5017
Garcia-Bonavila, Estela
Catalán, Jaime|||0000-0002-0878-7819
Papas, Marion|||0000-0001-8610-2734
Bonet, Sergi|||0000-0002-5125-7274
Miró, Jordi|||0000-0002-1085-0039
Yeste Oliveras, Marc|||0000-0002-2209-340X
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Acetazolamide
Aquaporins
Phloretin
Propanediol
Sperm
Stallion
topic Acetazolamide
Aquaporins
Phloretin
Propanediol
Sperm
Stallion
description Aquaporins (AQPs), a family of ubiquitous water channels divided into orthodox AQPs, aquaglyceroporins (GLPs), and superAQPs, are present in stallion spermatozoa. The aim of this study was to elucidate the functional relevance of each group of AQPs during stallion sperm cryopreservation through the use of three different inhibitors: acetazolamide (AC), phloretin (PHL) and propanediol (PDO). Sperm quality and function parameters were evaluated in the presence or absence of each inhibitor in fresh and frozen-thawed samples. In the presence of AC, different parameters were altered (p < 0.05), but not in a concentration-or time-depending manner. PHL was found to decrease sperm motility, viability, acrosome integrity, and the percentages of spermatozoa with low membrane lipid disorder, high mitochondrial membrane potential (MMP) and high intracellular levels of calcium and superoxides (p < 0.05). Finally, the sperm motility, viability, acrosome integrity, the percentages of spermatozoa with low membrane lipid disorder, high MMP and high intracellular calcium levels were higher (p < 0.05) in PDO treatments than in the control. The sperm response to AC, PHL and PDO indicates that GLPs, rather than orthodox AQPs, play a crucial role during stallion sperm cryopreservation. Furthermore, post-thaw sperm quality was higher in PDO treatments than in the control, suggesting that this molecule is a potential permeable cryoprotectant.
publishDate 2019
dc.date.none.fl_str_mv 2
2019-01-01
2019
2019-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/216719
https://dx.doi.org/urn:doi:10.3390/biology8040085
url https://ddd.uab.cat/record/216719
https://dx.doi.org/urn:doi:10.3390/biology8040085
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2017/SGR-1229
Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 AGL2017-88329-R
Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 PRE2018-083488
Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 RYC-2014-15581
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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