Cryotolerance of stallion spermatozoa relies on aquaglyceroporins rather than orthodox aquaporins

Aquaporins (AQPs), a family of ubiquitous water channels divided into orthodox AQPs, aquaglyceroporins (GLPs), and superAQPs, are present in stallion spermatozoa. The aim of this study was to elucidate the functional relevance of each group of AQPs during stallion sperm cryopreservation through the...

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Detalles Bibliográficos
Autores: Delgado Bermudez, Ariadna|||0000-0001-5655-5003, Noto, Federico, Bonilla Correal, Sebastian|||0000-0003-1015-5017, Garcia-Bonavila, Estela, Catalán, Jaime|||0000-0002-0878-7819, Papas, Marion|||0000-0001-8610-2734, Bonet, Sergi|||0000-0002-5125-7274, Miró, Jordi|||0000-0002-1085-0039, Yeste Oliveras, Marc|||0000-0002-2209-340X
Tipo de recurso: artículo
Fecha de publicación:2019
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:216719
Acceso en línea:https://ddd.uab.cat/record/216719
https://dx.doi.org/urn:doi:10.3390/biology8040085
Access Level:acceso abierto
Palabra clave:Acetazolamide
Aquaporins
Phloretin
Propanediol
Sperm
Stallion
Descripción
Sumario:Aquaporins (AQPs), a family of ubiquitous water channels divided into orthodox AQPs, aquaglyceroporins (GLPs), and superAQPs, are present in stallion spermatozoa. The aim of this study was to elucidate the functional relevance of each group of AQPs during stallion sperm cryopreservation through the use of three different inhibitors: acetazolamide (AC), phloretin (PHL) and propanediol (PDO). Sperm quality and function parameters were evaluated in the presence or absence of each inhibitor in fresh and frozen-thawed samples. In the presence of AC, different parameters were altered (p < 0.05), but not in a concentration-or time-depending manner. PHL was found to decrease sperm motility, viability, acrosome integrity, and the percentages of spermatozoa with low membrane lipid disorder, high mitochondrial membrane potential (MMP) and high intracellular levels of calcium and superoxides (p < 0.05). Finally, the sperm motility, viability, acrosome integrity, the percentages of spermatozoa with low membrane lipid disorder, high MMP and high intracellular calcium levels were higher (p < 0.05) in PDO treatments than in the control. The sperm response to AC, PHL and PDO indicates that GLPs, rather than orthodox AQPs, play a crucial role during stallion sperm cryopreservation. Furthermore, post-thaw sperm quality was higher in PDO treatments than in the control, suggesting that this molecule is a potential permeable cryoprotectant.

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