A singular PpaA/AerR-like protein in Rhodospirillum rubrum rules beyond the boundaries of photosynthesis in response to the intracellular redox state

21 p.-8 fig.

Detalles Bibliográficos
Autores: Godoy, Manuel S., De Miguel, Santiago R., Prieto, María Auxiliadora
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/341318
Acceso en línea:http://hdl.handle.net/10261/341318
Access Level:acceso abierto
Palabra clave:AerR/PpaA
Purple non-sulphur bacteria
Rhodospirillum rubrum
Photosynthesis
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spelling A singular PpaA/AerR-like protein in Rhodospirillum rubrum rules beyond the boundaries of photosynthesis in response to the intracellular redox stateGodoy, Manuel S.De Miguel, Santiago R.Prieto, María AuxiliadoraAerR/PpaAPurple non-sulphur bacteriaRhodospirillum rubrumPhotosynthesis21 p.-8 fig.Photosynthesis (PS) in purple non-Sulfur bacteria (PNSB) is strictly controlled to avoid energy consumption and to prevent oxidative stress when oxygen is present. PpsR, a transcriptional repressor encoded in the photosynthesis gene cluster (PGC), is one of the main players in the regulation of PS-related genes. In some cases, PpsR does not sense the environmental cue by itself but delegates this task to other proteins like PpaA/AerR anti-repressors, which can ultimately affect PpsR affinity to some promoter regions. In this work, the effects of locus Rru_A0625 product (HP1) on PS regulation of Rhodospirillum rubrum were studied by mutation/complementation and transcriptomic strategies. Rru_A0625 is located next to ppsR gene, just like other PpaA/AerR members, and its deletion annuls pigment synthesis in dark micro/anaerobic growth conditions. HP1 shows similarity to PpaA/AerR anti-repressors family, although it does not possess their typical cobalamin binding domain. A transcriptomic analysis of Rru_A0625 deletion mutant showed that HP1 not only has effects on bacteriochlorophyll and carotenoid biosynthesis, but also many other biological processes in the cell. The most notorious is the impact on the transcription of the nitrogenase complex components and accessory proteins. Our results suggest that this new member of the PpaA/AerR family has evolved losing the canonical cobalamin binding domain, but not the redox sensing capability, conserving a not fully understood mechanism of PS regulation.The authors want to acknowledge the financial support from the CSIC Interdisciplinary Thematic Platform (PTI+) Sustainable Plastics toward a Circular Economy (PTI-Susplast+), the Community of Madrid (P2018/NMT4389), and the Spanish Ministry of Science and Innovation under the research grant BIOCIR (PID2020-112766RB-C21).Peer reviewedAmerican Society for MicrobiologyConsejo Superior de Investigaciones Científicas (España)Comunidad de MadridMinisterio de Ciencia e Innovación (España)Godoy, Manuel S. [0000-0003-3754-4661]De Miguel, Santiago R. [0000-0001-7102-813X]Prieto, María Auxiliadora [0000-0002-8038-1223]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232023info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/341318reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#P2018/NMT4389-NANOBIOCARGO-CMinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-112766RB-C21https://doi.org/10.1128/msystems.00702-23Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3413182026-05-22T06:33:51Z
dc.title.none.fl_str_mv A singular PpaA/AerR-like protein in Rhodospirillum rubrum rules beyond the boundaries of photosynthesis in response to the intracellular redox state
title A singular PpaA/AerR-like protein in Rhodospirillum rubrum rules beyond the boundaries of photosynthesis in response to the intracellular redox state
spellingShingle A singular PpaA/AerR-like protein in Rhodospirillum rubrum rules beyond the boundaries of photosynthesis in response to the intracellular redox state
Godoy, Manuel S.
AerR/PpaA
Purple non-sulphur bacteria
Rhodospirillum rubrum
Photosynthesis
title_short A singular PpaA/AerR-like protein in Rhodospirillum rubrum rules beyond the boundaries of photosynthesis in response to the intracellular redox state
title_full A singular PpaA/AerR-like protein in Rhodospirillum rubrum rules beyond the boundaries of photosynthesis in response to the intracellular redox state
title_fullStr A singular PpaA/AerR-like protein in Rhodospirillum rubrum rules beyond the boundaries of photosynthesis in response to the intracellular redox state
title_full_unstemmed A singular PpaA/AerR-like protein in Rhodospirillum rubrum rules beyond the boundaries of photosynthesis in response to the intracellular redox state
title_sort A singular PpaA/AerR-like protein in Rhodospirillum rubrum rules beyond the boundaries of photosynthesis in response to the intracellular redox state
dc.creator.none.fl_str_mv Godoy, Manuel S.
De Miguel, Santiago R.
Prieto, María Auxiliadora
author Godoy, Manuel S.
author_facet Godoy, Manuel S.
De Miguel, Santiago R.
Prieto, María Auxiliadora
author_role author
author2 De Miguel, Santiago R.
Prieto, María Auxiliadora
author2_role author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas (España)
Comunidad de Madrid
Ministerio de Ciencia e Innovación (España)
Godoy, Manuel S. [0000-0003-3754-4661]
De Miguel, Santiago R. [0000-0001-7102-813X]
Prieto, María Auxiliadora [0000-0002-8038-1223]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv AerR/PpaA
Purple non-sulphur bacteria
Rhodospirillum rubrum
Photosynthesis
topic AerR/PpaA
Purple non-sulphur bacteria
Rhodospirillum rubrum
Photosynthesis
description 21 p.-8 fig.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/341318
url http://hdl.handle.net/10261/341318
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
P2018/NMT4389-NANOBIOCARGO-CM
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-112766RB-C21
https://doi.org/10.1128/msystems.00702-23
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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