Carbon roadmap from syngas to polyhydroxyalkanoates in Rhodospirillum rubrum
The gasification of organic waste materials to synthesis gas (syngas), followed by microbial fermentation provides a significant resource for generating bioproducts such as polyhydroxyalkanoates (PHA). The anaerobic photosynthetic bacterium, Rhodospirillum rubrum, is an organism particularly attract...
| Autores: | , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión aceptada para publicación |
| Fecha de publicación: | 2015 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/123622 |
| Acceso en línea: | http://hdl.handle.net/10261/123622 |
| Access Level: | acceso abierto |
| Palabra clave: | Rhodospirillum rubrum Polyhydroxyalkanoates Syngas CO metabolism CO assimilation |
| Sumario: | The gasification of organic waste materials to synthesis gas (syngas), followed by microbial fermentation provides a significant resource for generating bioproducts such as polyhydroxyalkanoates (PHA). The anaerobic photosynthetic bacterium, Rhodospirillum rubrum, is an organism particularly attractive for the bioconversion of syngas into PHAs. In this study, a quantitative physiological analysis of R. rubrum was carried out by implementing GC-MS and HPLC techniques to unravel the metabolic pathway operating during syngas fermentation that leads to PHA production. Further, detailed investigations of the central carbon metabolites using 13C-labeled substrate showed significant CO2 assimilation (of 40 %) into cell material and PHA from syngas carbon fraction. By a combination of quantitative gene expression and enzyme activity analyses, the main role of carboxylases from the central carbon metabolism in CO2 assimilation was shown, where the Calvin Benson-Bassham Cycle (CBB) played a minor role. This knowledge sheds light about the biochemical pathways that contribute to synthesis of PHA during syngas fermentation being valuable information to further optimize the fermentation process. |
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