TGF-β2, EGF, and FGF21 Growth Factors Present in Breast Milk Promote Mesenteric Lymph Node Lymphocytes Maturation in Suckling Rats

Breast milk, due to its large number of nutrients and bioactive factors, contributes to optimal development and immune maturation in early life. In this study, we aimed to assess the influence of some growth factors present in breast milk, such as transforming growth factor-β2 (TGF-β2), epidermal gr...

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Autores: Torres-Castro, Paulina, Abril Gil, Maria del Mar, Rodríguez Lagunas, María José, Castell, Margarida, Pérez-Cano, Francisco J., Franch i Masferrer, Àngels
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2018
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/124585
Acceso en línea:https://hdl.handle.net/2445/124585
Access Level:acceso abierto
Palabra clave:Llet materna
Citoquines
Limfòcits
Breast milk
Cytokines
Lymphocytes
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spelling TGF-β2, EGF, and FGF21 Growth Factors Present in Breast Milk Promote Mesenteric Lymph Node Lymphocytes Maturation in Suckling RatsTorres-Castro, PaulinaAbril Gil, Maria del MarRodríguez Lagunas, María JoséCastell, MargaridaPérez-Cano, Francisco J.Franch i Masferrer, ÀngelsLlet maternaCitoquinesLimfòcitsBreast milkCytokinesLymphocytesBreast milk, due to its large number of nutrients and bioactive factors, contributes to optimal development and immune maturation in early life. In this study, we aimed to assess the influence of some growth factors present in breast milk, such as transforming growth factor-β2 (TGF-β2), epidermal growth factor (EGF), and fibroblast growth factor 21 (FGF21), on the immune response development. Newborn Wistar rats were supplemented daily with TGF-β2, EGF, or FGF21, throughout the suckling period. At day 14 and 21 of life, lymphocytes from mesenteric lymph nodes (MLNs) were isolated, immunophenotyped, and cultured to evaluate their ability to proliferate and release cytokines. The main results demonstrated that supplementation with TGF-β2, EGF, or FGF21 modified the lymphocyte composition in MLNs. At day 14, all supplementations were able to induce a lower percentage of natural killer (NK) cells with the immature phenotype (CD8+), and they reduced the CD8αα/CD8αβ ratio at day 21. Moreover, the cytokine pattern was modified by the three treatments, with a down regulation of interleukin (IL)-13 secretion. These results showed the contribution of these growth factors in the lymphocytes MLNs immune maturation during the neonatal period. View Full-Text Keywords: growth factors; breast milk; immunonutrition; cytokines; lymphocytesMDPI2018info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/124585Articles publicats en revistes (Bioquímica i Fisiologia)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.3390/nu10091171Nutrients, 2018, vol. 10, num. 9, p. 1171https://doi.org/10.3390/nu10091171cc-by (c) Torres-Castro, Paulina et al., 2018http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1245852026-05-27T06:46:51Z
dc.title.none.fl_str_mv TGF-β2, EGF, and FGF21 Growth Factors Present in Breast Milk Promote Mesenteric Lymph Node Lymphocytes Maturation in Suckling Rats
title TGF-β2, EGF, and FGF21 Growth Factors Present in Breast Milk Promote Mesenteric Lymph Node Lymphocytes Maturation in Suckling Rats
spellingShingle TGF-β2, EGF, and FGF21 Growth Factors Present in Breast Milk Promote Mesenteric Lymph Node Lymphocytes Maturation in Suckling Rats
Torres-Castro, Paulina
Llet materna
Citoquines
Limfòcits
Breast milk
Cytokines
Lymphocytes
title_short TGF-β2, EGF, and FGF21 Growth Factors Present in Breast Milk Promote Mesenteric Lymph Node Lymphocytes Maturation in Suckling Rats
title_full TGF-β2, EGF, and FGF21 Growth Factors Present in Breast Milk Promote Mesenteric Lymph Node Lymphocytes Maturation in Suckling Rats
title_fullStr TGF-β2, EGF, and FGF21 Growth Factors Present in Breast Milk Promote Mesenteric Lymph Node Lymphocytes Maturation in Suckling Rats
title_full_unstemmed TGF-β2, EGF, and FGF21 Growth Factors Present in Breast Milk Promote Mesenteric Lymph Node Lymphocytes Maturation in Suckling Rats
title_sort TGF-β2, EGF, and FGF21 Growth Factors Present in Breast Milk Promote Mesenteric Lymph Node Lymphocytes Maturation in Suckling Rats
dc.creator.none.fl_str_mv Torres-Castro, Paulina
Abril Gil, Maria del Mar
Rodríguez Lagunas, María José
Castell, Margarida
Pérez-Cano, Francisco J.
Franch i Masferrer, Àngels
author Torres-Castro, Paulina
author_facet Torres-Castro, Paulina
Abril Gil, Maria del Mar
Rodríguez Lagunas, María José
Castell, Margarida
Pérez-Cano, Francisco J.
Franch i Masferrer, Àngels
author_role author
author2 Abril Gil, Maria del Mar
Rodríguez Lagunas, María José
Castell, Margarida
Pérez-Cano, Francisco J.
Franch i Masferrer, Àngels
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Llet materna
Citoquines
Limfòcits
Breast milk
Cytokines
Lymphocytes
topic Llet materna
Citoquines
Limfòcits
Breast milk
Cytokines
Lymphocytes
description Breast milk, due to its large number of nutrients and bioactive factors, contributes to optimal development and immune maturation in early life. In this study, we aimed to assess the influence of some growth factors present in breast milk, such as transforming growth factor-β2 (TGF-β2), epidermal growth factor (EGF), and fibroblast growth factor 21 (FGF21), on the immune response development. Newborn Wistar rats were supplemented daily with TGF-β2, EGF, or FGF21, throughout the suckling period. At day 14 and 21 of life, lymphocytes from mesenteric lymph nodes (MLNs) were isolated, immunophenotyped, and cultured to evaluate their ability to proliferate and release cytokines. The main results demonstrated that supplementation with TGF-β2, EGF, or FGF21 modified the lymphocyte composition in MLNs. At day 14, all supplementations were able to induce a lower percentage of natural killer (NK) cells with the immature phenotype (CD8+), and they reduced the CD8αα/CD8αβ ratio at day 21. Moreover, the cytokine pattern was modified by the three treatments, with a down regulation of interleukin (IL)-13 secretion. These results showed the contribution of these growth factors in the lymphocytes MLNs immune maturation during the neonatal period. View Full-Text Keywords: growth factors; breast milk; immunonutrition; cytokines; lymphocytes
publishDate 2018
dc.date.none.fl_str_mv 2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/124585
url https://hdl.handle.net/2445/124585
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.3390/nu10091171
Nutrients, 2018, vol. 10, num. 9, p. 1171
https://doi.org/10.3390/nu10091171
dc.rights.none.fl_str_mv cc-by (c) Torres-Castro, Paulina et al., 2018
http://creativecommons.org/licenses/by/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Torres-Castro, Paulina et al., 2018
http://creativecommons.org/licenses/by/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Articles publicats en revistes (Bioquímica i Fisiologia)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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