Do folate-receptor targeted liposomal photosensitizers enhance photodynamic therapy selectivity?

One of the current goals in photodynamic therapy research is to enhance the selective targeting of tumor cells in order to minimize the risk and the extension of unwanted side-effects caused by normal cell damage. Special attention is given to receptor mediated delivery systems, in particular, to th...

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Autores: Nonell, Santi, García Díaz, María, Sagristá, M. Lluïsa, Villanueva, Ángeles, Stockert, Juan Carlos, Cañete, Magdalena, Casadó, Ana, Mora, Margarita
Formato: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2011
País:España
Recursos:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:20.500.14342/1066
Acesso em linha:http://hdl.handle.net/20.500.14342/1066
https://doi.org/10.1016/j.bbamem.2010.12.014
Access Level:acceso abierto
Palavra-chave:Fotoquimioteràpia
Liposomes
Medicaments--Modes d'administració
Photodynamic therapy
Folate receptor
Targeted drug delivery
Singlet oxygen
577
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spelling Do folate-receptor targeted liposomal photosensitizers enhance photodynamic therapy selectivity?Nonell, SantiGarcía Díaz, MaríaSagristá, M. LluïsaVillanueva, ÁngelesStockert, Juan CarlosCañete, MagdalenaCasadó, AnaMora, MargaritaFotoquimioteràpiaLiposomesMedicaments--Modes d'administracióPhotodynamic therapyFolate receptorTargeted drug deliverySinglet oxygen577One of the current goals in photodynamic therapy research is to enhance the selective targeting of tumor cells in order to minimize the risk and the extension of unwanted side-effects caused by normal cell damage. Special attention is given to receptor mediated delivery systems, in particular, to those targeted to folate receptor. Incorporation of a model photosensitizer (ZnTPP) into a folate-targeted liposomal formulation has been shown to lead an uptake by HeLa cells (folate receptor positive cells) 2-fold higher than the non-targeted formulation. As a result, the photocytotoxicity induced by folate-targeted liposomes was improved. This selectivity was completely inhibited with an excess of folic acid present in the cell culture media. Moreover, A549 cells (folate receptor deficient cells) have not shown variations in the liposomal incorporation. Nevertheless, the differences observed were slighter than expected. Both folate-targeted and non-targeted liposomes localize in acidic lysosomes, which confirms that the non-specific adsorptive pathway is also involved. These results are consistent with the singlet oxygen kinetics measured in living cells treated with both liposomal formulations.ElsevierUniversitat Ramon Llull. IQS2011info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersion42 p.http://hdl.handle.net/20.500.14342/1066https://doi.org/10.1016/j.bbamem.2010.12.014reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésBiochimica et Biophysica Acta (BBA) -Biomembranes. Vol.1808, n.4 (2011), p.1063-1071info:eu-repo/grantAgreement/MICINN/PN+I/CTQ2007-67763-C03© Elsevier. Tots els drets reservatsinfo:eu-repo/semantics/openAccessoai:recercat.cat:20.500.14342/10662026-05-29T05:05:01Z
dc.title.none.fl_str_mv Do folate-receptor targeted liposomal photosensitizers enhance photodynamic therapy selectivity?
title Do folate-receptor targeted liposomal photosensitizers enhance photodynamic therapy selectivity?
spellingShingle Do folate-receptor targeted liposomal photosensitizers enhance photodynamic therapy selectivity?
Nonell, Santi
Fotoquimioteràpia
Liposomes
Medicaments--Modes d'administració
Photodynamic therapy
Folate receptor
Targeted drug delivery
Singlet oxygen
577
title_short Do folate-receptor targeted liposomal photosensitizers enhance photodynamic therapy selectivity?
title_full Do folate-receptor targeted liposomal photosensitizers enhance photodynamic therapy selectivity?
title_fullStr Do folate-receptor targeted liposomal photosensitizers enhance photodynamic therapy selectivity?
title_full_unstemmed Do folate-receptor targeted liposomal photosensitizers enhance photodynamic therapy selectivity?
title_sort Do folate-receptor targeted liposomal photosensitizers enhance photodynamic therapy selectivity?
dc.creator.none.fl_str_mv Nonell, Santi
García Díaz, María
Sagristá, M. Lluïsa
Villanueva, Ángeles
Stockert, Juan Carlos
Cañete, Magdalena
Casadó, Ana
Mora, Margarita
author Nonell, Santi
author_facet Nonell, Santi
García Díaz, María
Sagristá, M. Lluïsa
Villanueva, Ángeles
Stockert, Juan Carlos
Cañete, Magdalena
Casadó, Ana
Mora, Margarita
author_role author
author2 García Díaz, María
Sagristá, M. Lluïsa
Villanueva, Ángeles
Stockert, Juan Carlos
Cañete, Magdalena
Casadó, Ana
Mora, Margarita
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universitat Ramon Llull. IQS
dc.subject.none.fl_str_mv Fotoquimioteràpia
Liposomes
Medicaments--Modes d'administració
Photodynamic therapy
Folate receptor
Targeted drug delivery
Singlet oxygen
577
topic Fotoquimioteràpia
Liposomes
Medicaments--Modes d'administració
Photodynamic therapy
Folate receptor
Targeted drug delivery
Singlet oxygen
577
description One of the current goals in photodynamic therapy research is to enhance the selective targeting of tumor cells in order to minimize the risk and the extension of unwanted side-effects caused by normal cell damage. Special attention is given to receptor mediated delivery systems, in particular, to those targeted to folate receptor. Incorporation of a model photosensitizer (ZnTPP) into a folate-targeted liposomal formulation has been shown to lead an uptake by HeLa cells (folate receptor positive cells) 2-fold higher than the non-targeted formulation. As a result, the photocytotoxicity induced by folate-targeted liposomes was improved. This selectivity was completely inhibited with an excess of folic acid present in the cell culture media. Moreover, A549 cells (folate receptor deficient cells) have not shown variations in the liposomal incorporation. Nevertheless, the differences observed were slighter than expected. Both folate-targeted and non-targeted liposomes localize in acidic lysosomes, which confirms that the non-specific adsorptive pathway is also involved. These results are consistent with the singlet oxygen kinetics measured in living cells treated with both liposomal formulations.
publishDate 2011
dc.date.none.fl_str_mv 2011
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.14342/1066
https://doi.org/10.1016/j.bbamem.2010.12.014
url http://hdl.handle.net/20.500.14342/1066
https://doi.org/10.1016/j.bbamem.2010.12.014
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Biochimica et Biophysica Acta (BBA) -Biomembranes. Vol.1808, n.4 (2011), p.1063-1071
info:eu-repo/grantAgreement/MICINN/PN+I/CTQ2007-67763-C03
dc.rights.none.fl_str_mv © Elsevier. Tots els drets reservats
info:eu-repo/semantics/openAccess
rights_invalid_str_mv © Elsevier. Tots els drets reservats
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 42 p.
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.811543

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