DNA data storage in electrospun and melt-electrowritten composite nucleic acid-polymer fibers

Incorporating biomolecules as integral parts of computational systems represents a frontier challenge in bio- and nanotechnology. Using DNA to store digital data is an attractive alternative to conventional information technologies due to its high information density and long lifetime. However, deve...

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Authors: Soukarie, Diana, Nocete, Lluis, Bittner, Alexander M., Santiago, Ibon
Format: article
Publication Date:2024
Country:España
Institution:Universitat Autònoma de Barcelona
Repository:Dipòsit Digital de Documents de la UAB
Language:English
OAI Identifier:oai:ddd.uab.cat:311368
Online Access:https://ddd.uab.cat/record/311368
https://dx.doi.org/urn:doi:10.1016/j.mtbio.2023.100900
Access Level:Open access
Keyword:DNA data storage
Nanofibers
Melt-electrowriting
Electrospinning
Additive manufacturing
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spelling DNA data storage in electrospun and melt-electrowritten composite nucleic acid-polymer fibersSoukarie, DianaNocete, LluisBittner, Alexander M.Santiago, IbonDNA data storageNanofibersMelt-electrowritingElectrospinningAdditive manufacturingIncorporating biomolecules as integral parts of computational systems represents a frontier challenge in bio- and nanotechnology. Using DNA to store digital data is an attractive alternative to conventional information technologies due to its high information density and long lifetime. However, developing an adequate DNA storage medium remains a significant challenge in permitting the safe archiving and retrieval of oligonucleotides. This work introduces composite nucleic acid-polymer fibers as matrix materials for digital information-bearing oligonucleotides. We devised a complete workflow for the stable storage of DNA in PEO, PVA, and PCL fibers by employing electrohydrodynamic processes to produce electrospun nanofibers with embedded oligonucleotides. The on-demand retrieval of messages is afforded by non-hazardous chemical treatment and subsequent PCR amplification and DNA sequencing. Finally, we develop a platform for melt-electrowriting of polymer-DNA composites to produce microfiber meshes of programmable patterns and geometries. 22024-01-0120242024-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/311368https://dx.doi.org/urn:doi:10.1016/j.mtbio.2023.100900reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengMinisterio de Ciencia e Innovación https://doi.org/10.13039/501100004837 CEX2020-001038-MAgencia Estatal de Investigación https://doi.org/10.13039/501100011033 PID2019-104650GB-C22European Commission https://doi.org/10.13039/501100000780 101115292open accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.https://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:3113682026-06-06T12:50:31Z
dc.title.none.fl_str_mv DNA data storage in electrospun and melt-electrowritten composite nucleic acid-polymer fibers
title DNA data storage in electrospun and melt-electrowritten composite nucleic acid-polymer fibers
spellingShingle DNA data storage in electrospun and melt-electrowritten composite nucleic acid-polymer fibers
Soukarie, Diana
DNA data storage
Nanofibers
Melt-electrowriting
Electrospinning
Additive manufacturing
title_short DNA data storage in electrospun and melt-electrowritten composite nucleic acid-polymer fibers
title_full DNA data storage in electrospun and melt-electrowritten composite nucleic acid-polymer fibers
title_fullStr DNA data storage in electrospun and melt-electrowritten composite nucleic acid-polymer fibers
title_full_unstemmed DNA data storage in electrospun and melt-electrowritten composite nucleic acid-polymer fibers
title_sort DNA data storage in electrospun and melt-electrowritten composite nucleic acid-polymer fibers
dc.creator.none.fl_str_mv Soukarie, Diana
Nocete, Lluis
Bittner, Alexander M.
Santiago, Ibon
author Soukarie, Diana
author_facet Soukarie, Diana
Nocete, Lluis
Bittner, Alexander M.
Santiago, Ibon
author_role author
author2 Nocete, Lluis
Bittner, Alexander M.
Santiago, Ibon
author2_role author
author
author
dc.subject.none.fl_str_mv DNA data storage
Nanofibers
Melt-electrowriting
Electrospinning
Additive manufacturing
topic DNA data storage
Nanofibers
Melt-electrowriting
Electrospinning
Additive manufacturing
description Incorporating biomolecules as integral parts of computational systems represents a frontier challenge in bio- and nanotechnology. Using DNA to store digital data is an attractive alternative to conventional information technologies due to its high information density and long lifetime. However, developing an adequate DNA storage medium remains a significant challenge in permitting the safe archiving and retrieval of oligonucleotides. This work introduces composite nucleic acid-polymer fibers as matrix materials for digital information-bearing oligonucleotides. We devised a complete workflow for the stable storage of DNA in PEO, PVA, and PCL fibers by employing electrohydrodynamic processes to produce electrospun nanofibers with embedded oligonucleotides. The on-demand retrieval of messages is afforded by non-hazardous chemical treatment and subsequent PCR amplification and DNA sequencing. Finally, we develop a platform for melt-electrowriting of polymer-DNA composites to produce microfiber meshes of programmable patterns and geometries.
publishDate 2024
dc.date.none.fl_str_mv 2
2024-01-01
2024
2024-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/311368
https://dx.doi.org/urn:doi:10.1016/j.mtbio.2023.100900
url https://ddd.uab.cat/record/311368
https://dx.doi.org/urn:doi:10.1016/j.mtbio.2023.100900
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Ministerio de Ciencia e Innovación https://doi.org/10.13039/501100004837 CEX2020-001038-M
Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 PID2019-104650GB-C22
European Commission https://doi.org/10.13039/501100000780 101115292
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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